Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 544,385 G→T 100% A96S (GCA→TCA)  mntR → transcriptional regulator of mntH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE544,3850GT100.0% 113.7 / NA 33A96S (GCA→TCA) mntRtranscriptional regulator of mntH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (0/33);  total (0/33)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACGGCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTTGTTGG  >  minE/544382‑544451
   |                                                                  
aCGTCAGAAGGAGAGAAGCTGGCGCAGGACAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:369120/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGACAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:241287/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:571201/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:456967/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:464151/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:474617/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:51549/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:524038/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:549195/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:564580/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:451169/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:571783/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:61184/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:645885/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:654976/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:70265/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:72962/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:98841/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:455443/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:12985/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:447031/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:375717/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:374636/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:339540/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:295031/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:291087/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:26846/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:212670/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:207334/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:189374/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:17903/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:167369/70‑1 (MQ=255)
aCGTCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTtgttgg  <  1:145543/70‑1 (MQ=255)
   |                                                                  
ACGGCAGAAGGAGAGAAGCTGGCGCAGGAAAGCCGCGAGCGACATCAGATAGTCGAAAATTTCTTGTTGG  >  minE/544382‑544451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: