| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,669,352:1 | +AAC | 100% | coding (691/1473 nt) | der ← | predicted GTP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,669,352 | 1 | . | A | 100.0% | 17.5 / NA | 7 | Y231? (TAC→NAC) | der | predicted GTP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (0/7); total (0/7) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | minE | 1,669,352 | 2 | . | A | 100.0% | 17.5 / NA | 7 | Y231? (TAC→NAC) | der | predicted GTP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (0/7); total (0/7) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | minE | 1,669,352 | 3 | . | C | 100.0% | 19.6 / NA | 7 | Y231D (TAC→GAC) | der | predicted GTP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (0/7); total (0/7) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGTCACGCGTCGTGCCAGGCATGTCGTA‑‑‑AACAACAACGCGCTCTTCACCAAGAATACG > minE/1669325‑1669382 ||| tGTCACGCGTCGTGCCAGGCATGTCGTAAACAACAACAACGCGCTCTTCACCAAGAATACg < 1:150417/61‑1 (MQ=255) aCGCGTCGTGCCAGGCATGTCGTAAACAACAACAACGCGCTCTTCACCAAGAATACg < 1:101962/57‑1 (MQ=255) aCGCGTCGTGCCAGGCATGTCGTAAACAACAACAACGCGCTCTTCACCAAGAATACg < 1:44479/57‑1 (MQ=255) aCGCGTCGTGCCAGGCATGTCGTAAACAACAACAACGCGCTCTTCACCAAGAATACg < 1:515247/57‑1 (MQ=255) aCGCGTCGTGCCAGGCATGTCGTAAACAACAACAACGCGCTCTTCACCAAGAATACg < 1:555968/57‑1 (MQ=255) aCGCGTCGTGCCAGGCATGTCGTAAACAACAACAACGCGCTCTTCACCAAGAATACg < 1:574444/57‑1 (MQ=255) aCGCGTCGTGCCAGGCATGTCATAAACAACAACAACGCGCTCTTCACCAAGAATACg < 1:28112/57‑1 (MQ=255) ||| TGTCACGCGTCGTGCCAGGCATGTCGTA‑‑‑AACAACAACGCGCTCTTCACCAAGAATACG > minE/1669325‑1669382 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |