Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 52,517 T→C 100% E757E (GAA→GAG)  imp ← exported protein required for envelope biosynthesis and integrity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE52,5170TC77.8% 13.1 / 0.3 9E757E (GAA→GAG) impexported protein required for envelope biosynthesis and integrity
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (4/3);  total (4/5)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGT  >  minE/52465‑52559
                                                    |                                          
aaCGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGtt                                    <  1:183499/61‑1 (MQ=255)
aaCGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGtt                                    <  1:33203/61‑1 (MQ=255)
         tctcTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGAtt                          >  1:31231/1‑62 (MQ=255)
         tctcTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGAtt                          >  1:398978/1‑62 (MQ=255)
         tctcTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGAtt                          >  1:533345/1‑62 (MQ=255)
         tctcTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGAtt                          >  1:554175/1‑62 (MQ=255)
                                 tGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCAtgttgt  <  1:302385/62‑1 (MQ=255)
                                 tGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCAtgttgt  <  1:305209/62‑1 (MQ=255)
                                 tGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCAtgttgt  <  1:536585/62‑1 (MQ=255)
                                                    |                                          
AACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGT  >  minE/52465‑52559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑