Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 1,668,114 | G→A | 100% | L399L (CTG→CTA) | xseA → | exonuclease VII, large subunit |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 1,668,114 | 0 | G | A | 87.5% | 16.6 / ‑2.9 | 8 | L399L (CTG→CTA) | xseA | exonuclease VII, large subunit |
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (3/4); total (3/5) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TTTCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTGTCAACGCTGGCGCGTGGAT > minE/1668072‑1668133 | tttCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTGTCAACGCTGGCGCGTGGAt < 1:371849/62‑1 (MQ=255) tttCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTATCAACGCTGGCGCGTGGAt < 1:212336/62‑1 (MQ=255) tttCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTATCAACGCTGGCGCGTGGAt < 1:371267/62‑1 (MQ=255) tttCGGTAATGCAGTAACGCACCTCAAAGCCGTAAGCCCACTATCAACGCTGGCGCGTGGAt < 1:550226/62‑1 (MQ=255) ttCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTATCAACGCTGGCGCGTGGa > 1:215913/1‑60 (MQ=255) ttCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTATCAACGCTGGCGCGTGGa > 1:321453/1‑60 (MQ=255) ttCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTATCAACGCTGGCGCGTGGa > 1:339810/1‑60 (MQ=255) ttCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTATCAACGCTGGCGCGTGGAt < 1:241192/61‑1 (MQ=255) | TTTCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTGTCAACGCTGGCGCGTGGAT > minE/1668072‑1668133 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |