Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,668,114 G→A 100% L399L (CTG→CTA xseA → exonuclease VII, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,668,1140GA87.5% 16.6 / ‑2.9 8L399L (CTG→CTAxseAexonuclease VII, large subunit
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (3/4);  total (3/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTGTCAACGCTGGCGCGTGGAT  >  minE/1668072‑1668133
                                          |                   
tttCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTGTCAACGCTGGCGCGTGGAt  <  1:371849/62‑1 (MQ=255)
tttCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTATCAACGCTGGCGCGTGGAt  <  1:212336/62‑1 (MQ=255)
tttCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTATCAACGCTGGCGCGTGGAt  <  1:371267/62‑1 (MQ=255)
tttCGGTAATGCAGTAACGCACCTCAAAGCCGTAAGCCCACTATCAACGCTGGCGCGTGGAt  <  1:550226/62‑1 (MQ=255)
 ttCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTATCAACGCTGGCGCGTGGa   >  1:215913/1‑60 (MQ=255)
 ttCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTATCAACGCTGGCGCGTGGa   >  1:321453/1‑60 (MQ=255)
 ttCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTATCAACGCTGGCGCGTGGa   >  1:339810/1‑60 (MQ=255)
 ttCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTATCAACGCTGGCGCGTGGAt  <  1:241192/61‑1 (MQ=255)
                                          |                   
TTTCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTGTCAACGCTGGCGCGTGGAT  >  minE/1668072‑1668133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: