Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,668,114	G→A	100%	L399L (CTG→CTA)	xseA →	exonuclease VII, large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,668,114	0	G	A	87.5%	16.6 / ‑2.9	8	L399L (CTG→CTA)	xseA	exonuclease VII, large subunit
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (3/4);  total (3/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTGTCAACGCTGGCGCGTGGAT  >  minE/1668072‑1668133                                           |                    tttCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTGTCAACGCTGGCGCGTGGAt  <  1:371849/62‑1 (MQ=255) tttCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTATCAACGCTGGCGCGTGGAt  <  1:212336/62‑1 (MQ=255) tttCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTATCAACGCTGGCGCGTGGAt  <  1:371267/62‑1 (MQ=255) tttCGGTAATGCAGTAACGCACCTCAAAGCCGTAAGCCCACTATCAACGCTGGCGCGTGGAt  <  1:550226/62‑1 (MQ=255)  ttCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTATCAACGCTGGCGCGTGGa   >  1:215913/1‑60 (MQ=255)  ttCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTATCAACGCTGGCGCGTGGa   >  1:321453/1‑60 (MQ=255)  ttCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTATCAACGCTGGCGCGTGGa   >  1:339810/1‑60 (MQ=255)  ttCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTATCAACGCTGGCGCGTGGAt  <  1:241192/61‑1 (MQ=255)                                           |                    TTTCGGTAATGCAGTAACGCACCTCGAAGCCGTAAGCCCACTGTCAACGCTGGCGCGTGGAT  >  minE/1668072‑1668133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑