| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,043,667 | A→T | 100% | R31W (AGG→TGG) | yqiK → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,043,667 | 0 | A | T | 100.0% | 26.1 / NA | 10 | R31W (AGG→TGG) | yqiK | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (0/10); total (0/10) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAG > minE/2043631‑2043693 | gTATGCATTCTGTTTATTA‑TAGAATTATTTTCGCCTGGCTCTATCGTCGCGCTTCGGCagag < 1:231338/62‑1 (MQ=255) tATGCATTCTGTTTATTATTGGAATTATTTTCGCCTGGCTCTATCGTCGCGCTTCGGCagag < 1:147906/62‑1 (MQ=255) tATGCATTCTGTTTATTATTGGAATTATTTTCGCCTGGCTCTATCGTCGCGCTTCGGCagag < 1:334893/62‑1 (MQ=255) tATGCATTCTGTTTATTATTGGAATTATTTTCGCCTGGCTCTATCGTCGCGCTTCGGCagag < 1:379084/62‑1 (MQ=255) tATGCATTCTGTTTATTATTGGAATTATTTTCGCCTGGCTCTATCGTCGCGCTTCGGCagag < 1:396867/62‑1 (MQ=255) tATGCATTCTGTTTATTATTGGAATTATTTTCGCCTGGCTCTATCGTCGCGCTTCGGCagag < 1:467367/62‑1 (MQ=255) tATGCATTCTGTTTATTATTGGAATTATTTTCGCCTGGCTCTATCGTCGCGCTTCGGCagag < 1:48336/62‑1 (MQ=255) tATGCATTCTGTTTATTATTGGAATTATTTTCGCCTGGCTCTATCGTCGCGCTTCGGCagag < 1:537020/62‑1 (MQ=255) tATGCATTCTGTTTATTATTGGAATTATTTTCGCCTGGCTCTATCGTCGCGCTTCGGCagag < 1:64218/62‑1 (MQ=255) tATGCATTCTGTTTATTATTGGAATTATCTTCGCCTGGCTCTATCGTCGCGCTTCGGCagag < 1:574052/62‑1 (MQ=255) | GTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAG > minE/2043631‑2043693 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |