| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,340,208 | A→G | 40.0% | intergenic (‑2/‑371) | bipA ← / → glnA | GTP‑binding protein/glutamine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,340,208 | 0 | A | G | 40.0% | 17.0 / 31.2 | 30 | intergenic (‑2/‑371) | bipA/glnA | GTP‑binding protein/glutamine synthetase |
| Reads supporting (aligned to +/- strand): ref base A (5/13); new base G (5/7); total (10/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
ATGGTCTACGTGCGCGATGATGGCGATATTACGCAATTTTTCGATCACAACTTTGCCTCAGGCATTAGAAATAGCGCGTTATTGTACACGGATTAATCGCACTACAA > minE/2340159‑2340265 | aTGGTCTACGTGCGCGATGATGGCGATATTACGCAATTTTTCGATCACAACTTTGCCTCAg < 1:583442/61‑1 (MQ=255)aTGGTCTACGTGCGCGATGATGGCGATATTACGCAATTTTTCGATCACAACTTTGCCTCAg < 1:581521/61‑1 (MQ=255)aTGGTCTACGTGCGCGATGATGGCGATATTACGCAATTTTTCGATCACAACTTTGCCTCAg < 1:75264/61‑1 (MQ=255)aTGGTCTACGTGCGCGATGATGGCGATATTACGCAATTTTTCGATCACAACTTTGCCTCAg < 1:274348/61‑1 (MQ=255) gTCTACGTGCGCGATGATGGCGATATTACGCAATTTTTCGATCACAACTTTGCCTCAgg < 1:437378/59‑1 (MQ=255) tGCGCGATGATGGCGATATTACGCAATTTTTCGATCACAACTTTGCCTCAGGCATTAGAAAt < 1:19580/62‑1 (MQ=255) tGCGCGATGATGGCGATATTACGCAATTTTTCGATCACAACTTTGCCTCAGGCATTAGAAAt < 1:263907/62‑1 (MQ=255) cgATGATGGCGATATTACGCAATTTTTCGATCACAACTTTGCCTCAGGCATTAGAAATAgc < 1:1749/61‑1 (MQ=255) cgATGATGGCGATATTACGCAATTTTTCGATCACAACTTTGCCTCAGGCATTAGAAATAgc < 1:233898/61‑1 (MQ=255) cgATGATGGCGATATTACGCAATTTTTCGATCACAACTTTGCCTCAGGCATTAGAAATAgc < 1:294785/61‑1 (MQ=255) cgATGATGGCGATATTACGCAATTTTTCGATCACAACTTTGCCTCAGGCATTAGAAATAgc < 1:304669/61‑1 (MQ=255) gatgatGGCGATATTACGCAATTTTTCGATCACAACTTTGCCTCAGGCATTAGAAATAgcgc < 1:307598/62‑1 (MQ=255) gatgatGGCGATATTACGCAATTTTTCGATCACAACTTTGCCTCAGGCATTAGAAATAgcgc < 1:74817/62‑1 (MQ=255) gatgGCGATATTACGCAATTTTTCGATCACAGCTTTGCCTCAgg < 1:339853/44‑1 (MQ=255) gatgGCGATATTACGCAATTTTTCGATCACAGCTTTGCCTCAgg < 1:113957/44‑1 (MQ=255) gatgGCGATATTACGCAATTTTTCGATCACAGCTTTGCCTCAgg < 1:419398/44‑1 (MQ=255) gatgGCGATATTACGCAATTTTTCGATCACAGCTTTGCCTCAgg < 1:542370/44‑1 (MQ=255) gatgGCGATATTACGCAATTTTTCGATCACAGCTTTGCCTCAgg < 1:577517/44‑1 (MQ=255) gatgGCGATATTACGCAATTTTTCGATCACAGCTTTGCCTCAgg < 1:182678/44‑1 (MQ=255) gatgGCGATATTACGCAATTTTTCGATCACAGCTTTGCCTCAgg < 1:158544/44‑1 (MQ=255) atatTACGCAATTTTTCGATCACAGCTTTGCCTCAGGCATTAGa > 1:214680/1‑44 (MQ=255) atatTACGCAATTTTTCGATCACAGCTTTGCCTCAGGCATTAGa > 1:409409/1‑44 (MQ=255) atatTACGCAATTTTTCGATCACAGCTTTGCCTCAGGCATTAGa > 1:194469/1‑44 (MQ=255) atatTACGCAATTTTTCGATCACAGCTTTGCCTCAGGCATTAGa > 1:183746/1‑44 (MQ=255) atatTACGCAATTTTTCGATCACAGCTTTGCCTCAGGCATTAGa > 1:518916/1‑44 (MQ=255) cacaACTTTGCCTCAGGCATTAGAAATAGCGCGTTATTGTACACGGATTAATCGCACTACaa > 1:76602/1‑62 (MQ=255) cacaACTTTGCCTCAGGCATTAGAAATAGCGCGTTATTGTACACGGATTAATCGCACTACaa > 1:585898/1‑62 (MQ=255) cacaACTTTGCCTCAGGCATTAGAAATAGCGCGTTATTGTACACGGATTAATCGCACTACaa > 1:478403/1‑62 (MQ=255) cacaACTTTGCCTCAGGCATTAGAAATAGCGCGTTATTGTACACGGATTAATCGCACTACaa > 1:354240/1‑62 (MQ=255) cacaACTTTGCCTCAGGCATTAGAAATAGCGCGTTATTGTACACGGATTAATCGCACTACa > 1:350629/1‑61 (MQ=255) | ATGGTCTACGTGCGCGATGATGGCGATATTACGCAATTTTTCGATCACAACTTTGCCTCAGGCATTAGAAATAGCGCGTTATTGTACACGGATTAATCGCACTACAA > minE/2340159‑2340265 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |