Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,364,103 A→G 100% W456R (TGG→CGG)  trkH ← potassium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,364,1030AG81.8% 21.0 / 0.1 11W456R (TGG→CGG) trkHpotassium transporter
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (3/6);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 1.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGCCCCAG  >  minE/2364052‑2364163
                                                   |                                                            
gCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCAcc                                                    <  1:190995/62‑1 (MQ=255)
gCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCAcc                                                    <  1:5958/62‑1 (MQ=255)
             cTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGc                                                        >  1:426322/1‑45 (MQ=255)
                               cATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCa                     <  1:19326/62‑1 (MQ=255)
                               cATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCa                     >  1:24753/1‑62 (MQ=255)
                               cATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCa                     >  1:554168/1‑62 (MQ=255)
                               cATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCa                     <  1:567871/62‑1 (MQ=255)
                                               aTCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGccc     <  1:326505/62‑1 (MQ=255)
                                               aTCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGccc     <  1:380809/62‑1 (MQ=255)
                                                  cATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGCCCCAg  >  1:358702/1‑62 (MQ=255)
                                                  cATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGCCCCAg  >  1:495174/1‑62 (MQ=255)
                                                   |                                                            
GCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGCCCCAG  >  minE/2364052‑2364163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑