Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,371,447 A→G 100% F214L (TTT→CTT)  fre ← flavin reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,371,4470AG90.1% 57.5 / ‑1.5 20F214L (TTT→CTT) freflavin reductase
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (4/14);  total (6/14)
Fisher's exact test for biased strand distribution p-value = 7.89e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGG  >  minE/2371398‑2371485
                                                 |                                      
aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAACAGATCGCGGGc                            >  1:9121/1‑62 (MQ=255)
aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAACAGATCGCGGGc                            >  1:476671/1‑62 (MQ=255)
                     tCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc        >  1:183684/1‑61 (MQ=255)
                     tCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACg       >  1:217150/1‑62 (MQ=255)
                     tCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACg       >  1:321592/1‑62 (MQ=255)
                     tCTTCACGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCAtc               >  1:322233/1‑54 (MQ=255)
                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGt      <  1:32877/57‑1 (MQ=255)
                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGt      <  1:452819/57‑1 (MQ=255)
                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGt      <  1:188715/57‑1 (MQ=255)
                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGt      <  1:65400/57‑1 (MQ=255)
                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGt      <  1:587175/57‑1 (MQ=255)
                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGt      <  1:63810/57‑1 (MQ=255)
                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTcc    <  1:79496/59‑1 (MQ=255)
                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCgg  <  1:93009/61‑1 (MQ=255)
                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCgg  <  1:201712/61‑1 (MQ=255)
                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCgg  <  1:122051/61‑1 (MQ=255)
                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCgg  <  1:547541/61‑1 (MQ=255)
                           cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCgg  <  1:359738/61‑1 (MQ=255)
                            gcgcATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGt      <  1:564794/56‑1 (MQ=255)
                            gcgcATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCgg  <  1:527454/60‑1 (MQ=255)
                                                 |                                      
AACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGG  >  minE/2371398‑2371485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑