| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,620,805 | A→G | 31.6% | intergenic (‑67/+7) | zntA ← / ← yhhN | zinc, cobalt and lead efflux system/conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,620,805 | 0 | A | G | 31.6% | 20.7 / 11.2 | 19 | intergenic (‑67/+7) | zntA/yhhN | zinc, cobalt and lead efflux system/conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (8/5); new base G (3/3); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTTTTCTCATTAACCGAAGGATACACTCTGGAGTCGACTCCAGAGTCAAGTTTTATCAGAGATACAGCGAGCGGACGATCAGGAAGTGACCGGCAAAGTAGCAGG > minE/2620756‑2620861 | ttttttCTCATTAACCGAAGGATACACTCTGGAGTCGACTCCAGAGTCAAGTTTTATCagag < 1:57050/62‑1 (MQ=255) tttCTCATTAACCGAAGGATACACTCTGGAGTCGACTCCAGAGTCAGGTTTTATCagag > 1:417312/1‑59 (MQ=255) tttCTCATTAACCGAAGGATACACTCTGGAGTCGACTCCAGAGTCAGGTTTTATCAgaga > 1:526814/1‑60 (MQ=255) tttCTCATTAACCGAAGGATACACTCTGGAGTCGACTCCAGAGTCAGGTTTTATCAgaga > 1:513614/1‑60 (MQ=255) tAACCGAAGGATACACTCTGGAGTCGACTCCAGAGTCAAGTTTTATCAGAGATACagcgag < 1:245552/61‑1 (MQ=255) tAACCGAAGGATACACTCTGGAGTCGACTCCAGAGTCAAGTTTTATCAGAGATACagcgag < 1:279634/61‑1 (MQ=255) aGGATACACTCTGGAGTCGACTCCAGAGTCAAGTTTTATCag > 1:92417/1‑42 (MQ=255) aGGATACACTCTGGAGTCGACTCCAGAGTCAAGTTTTATCag > 1:231789/1‑42 (MQ=255) aGGATACACTCTGGAGTCGACTCCAGAGTCAAGTTTTATCag > 1:271956/1‑42 (MQ=255) aGGATACACTCTGGAGTCGACTCCAGAGTCAAGTTTTATCag > 1:273385/1‑42 (MQ=255) aGGATACACTCTGGAGTCGACTCCAGAGTCAAGTTTTATCag > 1:478752/1‑42 (MQ=255) tACACTCTGGAGTCGACTCCAGAGTCAAGTTTTATCAGAGATACAGCGAGCGGACg < 1:306360/56‑1 (MQ=255) tctGGAGTCGACTCCAGAGTCAAGTTTTATCAGAGATACAGCGAGCGGACGATCAGGAAGTg > 1:278606/1‑62 (MQ=255) tctGGAGTCGACTCCAGAGTCAAGTTTTATCAGAGATACAGCGACCGGACGATCAg > 1:447777/1‑56 (MQ=255) cGACTCCAGAGTCAAGTTTTATCAGAGCTACAGCGAGCGGACGATCAGGAAGTGACCGGc > 1:256310/1‑60 (MQ=255) gagTCAAGTTTTATCAGAGATACAGCGAGCGGACg < 1:189641/35‑1 (MQ=255) agTCAGGTTTTATCAGAGATACAGCGAGCGGACGATCAGGAAGTGACCGGCAAAGTAGCAgg < 1:41732/62‑1 (MQ=255) agTCAGGTTTTATCAGAGATACAGCGAGCGGACGATCAGGAAGTGACCGGCAAAGTAGCAgg < 1:33824/62‑1 (MQ=255) agTCAGGTTTTATCAGAGATACAGCGAGCGGACGATCAGGAAGTGACCGGCAAAGTAGCAgg < 1:200259/62‑1 (MQ=255) | TTTTTTCTCATTAACCGAAGGATACACTCTGGAGTCGACTCCAGAGTCAAGTTTTATCAGAGATACAGCGAGCGGACGATCAGGAAGTGACCGGCAAAGTAGCAGG > minE/2620756‑2620861 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |