| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,858,035 | C→A | 100% | T103T (ACC→ACA) | amiB → | N‑acetylmuramoyl‑l‑alanine amidase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,858,035 | 0 | C | A | 100.0% | 10.6 / NA | 5 | T103T (ACC→ACA) | amiB | N‑acetylmuramoyl‑l‑alanine amidase II |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (0/5); total (0/5) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTACGGCTGGTGGTCGATCTTACCGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGG > minE/2858012‑2858073 | cTACGGCTGGTGGTCGATCTTACAGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATgg < 1:411681/62‑1 (MQ=255)cTACGGCTGGTGGTCGATCTTACAGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATgg < 1:430701/62‑1 (MQ=255)cTACGGCTGGTGGTCGATCTTACAGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATgg < 1:509349/62‑1 (MQ=255)cTACGGCTGGTGGTCGATCTTACAGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATgg < 1:556210/62‑1 (MQ=255) tACGGCTGGTGGTCGATCTTACAGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATgg < 1:216120/61‑1 (MQ=255) | CTACGGCTGGTGGTCGATCTTACCGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGG > minE/2858012‑2858073 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |