Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,951,204 T→C 50.0% L250L (TTA→TTG)  fhuF ← ferric iron reductase involved in ferric hydroximate transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,951,2040TC50.0% ‑6.4 / 7.9 8L250L (TTA→TTG) fhuFferric iron reductase involved in ferric hydroximate transport
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base C (2/2);  total (4/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCAGTGCTTTCATTTCAGCGTACAATCGCCACATTGCTGCACATCCGGTAAGCGATAACGCTGGCAGCAAGTGCGGCGCACCAGCAGGCCGTCGCGCAGTACC  >  minE/2951155‑2951258
                                                 |                                                      
ggCAGTGCTTTCATTTCAGCGTACAATCGCCACATTGCTGCACATCCGGTAAGCGATAACGc                                            >  1:349650/1‑62 (MQ=255)
 gCAGTGCTTTCATTTCAGCGTACAATCGCCACATTGCTGCACATCCGGCAAGCGATAACGCt                                           <  1:434816/62‑1 (MQ=255)
  cAGTGCTTTCATTTCAGCGTACAATCGCCACATTGCTGCACATCCGGCAAGCGATAACGCt                                           <  1:347712/61‑1 (MQ=255)
   aGTGCTTTCATTTCAGCGTACAATCGCCACATTGCTGCACATCCGGTAAGCGATAACGCTgg                                         <  1:301639/62‑1 (MQ=255)
                               acaTTGCTGCACATCCGGTAAGCGATAACGCTGGCAGCAAGTGCGGCGCACCAGCAGGCCGt             <  1:210880/62‑1 (MQ=255)
                                          caTCCGGTAAGCGATAACGCTGGCAGCAAGTGCGGCGCACCAGCAGGCCGTCGCGCAGTAc   >  1:540346/1‑61 (MQ=255)
                                          caTCCGGCAAGCGATAACGCTGGCAGCAAGTGCGGCGCACCAGCAGGCCGTCGCGCAg      >  1:198097/1‑58 (MQ=255)
                                          caTCCGGCAAGCGATAACGCTGGCAGCAAGTGCGGCGCACCAGCAGGCCGTCGCGCAGTAcc  >  1:78721/1‑62 (MQ=255)
                                                 |                                                      
GGCAGTGCTTTCATTTCAGCGTACAATCGCCACATTGCTGCACATCCGGTAAGCGATAACGCTGGCAGCAAGTGCGGCGCACCAGCAGGCCGTCGCGCAGTACC  >  minE/2951155‑2951258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑