| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 636,766 | A→G | 100% | I1248V (ATT→GTT) | mukB → | fused chromosome partitioning proteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 636,766 | 0 | A | G | 90.9% | 28.1 / ‑3.1 | 11 | I1248V (ATT→GTT) | mukB | fused chromosome partitioning proteins |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (10/0); total (11/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCCGTGAACAGAAACTGGCGATCAGTTCCCGCAGCGTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGG > minE/636707‑636827 | cccGTGAACAGAAACTGGCGATCAGTTCCCGCAGCGTGGCGAACATCATTCGCAAAACCa > 1:137348/1‑60 (MQ=255)cccGTGAACAGAAACTGGCGATCAGTTCCCGCAGCGTGGCGAACATCATTCGCAAAACCa > 1:567955/1‑60 (MQ=255)cccGTGAACAGAAACTGGCGATCAGTTCCCGCAGCGTGGCGAACATCATTCGCAAAACCa > 1:366250/1‑60 (MQ=255)cccGTGAACAGAAACTGGCGATCAGTTCCCGCAGCGTGGCGAACATCATTCGCAAAACCa > 1:304686/1‑60 (MQ=255)cccGTGAACAGAAACTGGCGATCAGTTCCCGCAGCGTGGCGAACATCATTCGCAAAACCa > 1:248731/1‑60 (MQ=255) cAGTTCCCGCAGCGTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAAccg > 1:11891/1‑57 (MQ=255) cGTGGCGAACATCATTCGCAAAACCGTTCAGCGCGAGCAGAACCGTATCCGTATGCTCAAcc > 1:169819/1‑62 (MQ=255) cGTGGCGAACATCATTCGCAAAACCGTTCAGCGCGAGCAGAACCGTATCCGTATGCTCAAcc > 1:309341/1‑62 (MQ=255) cGTGGCGAACATCATTCGCAAAACCGTTCAGCGCGAGCAGAACCGTATCCGTATGCTCAAcc > 1:312511/1‑62 (MQ=255) cGTGGCGAACATCATTCGCAAAACCGTTCAGCGCGAGCAGAACCGTATCCGTATGCTCAAcc > 1:316189/1‑62 (MQ=255) cGTGGCGAACATCATTCGCAAAACCGTTCAGCGCGAGCAGAACCGTATCCGTATGCTCAAcc > 1:137688/1‑62 (MQ=255) cGTGGCGAACATCATTCGCAAAACCGTTCAGCGCGAGCAGAACCGTATCCGTATGCTCAAcc > 1:398821/1‑62 (MQ=255) cGTGGCGAACATCATTCGCAAAACCGTTCAGCGCGAGCAGAACCGTATCCGTATGCTCAAcc > 1:447948/1‑62 (MQ=255) cGTGGCGAACATCATTCGCAAAACCGTTCAGCGCGAGCAGAACCGTATCCGTATGCTCAAcc > 1:506222/1‑62 (MQ=255) cGTGGCGAACATCATTCGCAAAACCGTTCAGCGCGAGCAGAACCGTATCCGTATGCTCAAcc > 1:547837/1‑62 (MQ=255) cGTGGCGAACATCATTCGCAAAACCGTTCAGCGCGAGCAGAACCGTATCCGTATGCTCAAcc > 1:567424/1‑62 (MQ=255) aTTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTgg < 1:261681/62‑1 (MQ=255) aTTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTgg < 1:383697/62‑1 (MQ=255) aTTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTgg < 1:453827/62‑1 (MQ=255) | CCCGTGAACAGAAACTGGCGATCAGTTCCCGCAGCGTGGCGAACATCATTCGCAAAACCATTCAGCGCGAGCAGAACCGTATCCGTATGCTCAACCAGGGGTTGCAGAACGTATCGTTTGG > minE/636707‑636827 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |