| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 719,422 | T→C | 100% | I252I (ATT→ATC) | mviN → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 719,422 | 0 | T | C | 85.7% | 13.1 / ‑2.9 | 7 | I252I (ATT→ATC) | mviN | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (6/0); total (6/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACGCCGACCG > minE/719409‑719475 | tcatcaACACCATCTTCGCCTCGTTCCTGGCAGCCGGTTCGGTGTCCTGGATGtactac > 1:152587/1‑59 (MQ=255)tcatcaACACCATCTTCGCCTCGTTCCTGGCAGCCGGTTCGGTGTCCTGGATGtactac > 1:171228/1‑59 (MQ=255)tcatcaACACCATCTTCGCCTCGTTCCTGGCAGCCGGTTCGGTGTCCTGGATGtactac > 1:183414/1‑59 (MQ=255)tcatcaACACCATCTTCGCCTCGTTCCTGGCAGCCGGTTCGGTGTCCTGGATGtactac > 1:246384/1‑59 (MQ=255)tcatcaACACCATCTTCGCCTCGTTCCTGGCAGCCGGTTCGGTGTCCTGGATGtactac > 1:247073/1‑59 (MQ=255)tcatcaACACCATCTTCGCCTCGTTCCTGGCAGCCGGTTCGGTGTCCTGGATGtactac > 1:443465/1‑59 (MQ=255) aTTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACGCCGACCg < 1:449070/56‑1 (MQ=255) | TCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACGCCGACCG > minE/719409‑719475 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |