Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 719,425 T→C 100% F253F (TTT→TTC)  mviN → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE719,4250TC85.7% 11.5 / ‑1.9 7F253F (TTT→TTC) mviNpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (6/0);  total (6/1)
Fisher's exact test for biased strand distribution p-value = 1.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACGCCGACCG  >  minE/719409‑719475
                |                                                  
tcatcaACACCATCTTCGCCTCGTTCCTGGCAGCCGGTTCGGTGTCCTGGATGtactac          >  1:152587/1‑59 (MQ=255)
tcatcaACACCATCTTCGCCTCGTTCCTGGCAGCCGGTTCGGTGTCCTGGATGtactac          >  1:171228/1‑59 (MQ=255)
tcatcaACACCATCTTCGCCTCGTTCCTGGCAGCCGGTTCGGTGTCCTGGATGtactac          >  1:183414/1‑59 (MQ=255)
tcatcaACACCATCTTCGCCTCGTTCCTGGCAGCCGGTTCGGTGTCCTGGATGtactac          >  1:246384/1‑59 (MQ=255)
tcatcaACACCATCTTCGCCTCGTTCCTGGCAGCCGGTTCGGTGTCCTGGATGtactac          >  1:247073/1‑59 (MQ=255)
tcatcaACACCATCTTCGCCTCGTTCCTGGCAGCCGGTTCGGTGTCCTGGATGtactac          >  1:443465/1‑59 (MQ=255)
           aTTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACGCCGACCg  <  1:449070/56‑1 (MQ=255)
                |                                                  
TCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACGCCGACCG  >  minE/719409‑719475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑