Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	726,335	A→G	100%	D96G (GAC→GGC)	yceD →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	726,335	0	A	G	100.0%	38.7 / NA	12	D96G (GAC→GGC)	yceD	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/2);  total (10/2)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAAC  >  minE/726310‑726384                          |                                                  tATTGTTTTAGTCCTGTGCGTTCAGGCGAACAGGCTGAAGCAc                                  >  1:135681/1‑43 (MQ=255) tATTGTTTTAGTCCTGTGCGTTCAGGCGAACAGGCTGAAGCAc                                  >  1:200510/1‑43 (MQ=255) tATTGTTTTAGTCCTGTGCGTTCAGGCGAACAGGCTGAAGCAc                                  >  1:275761/1‑43 (MQ=255) tATTGTTTTAGTCCTGTGCGTTCAGGCGAACAGGCTGAAGCAc                                  >  1:37955/1‑43 (MQ=255) tATTGTTTTAGTCCTGTGCGTTCAGGCGAACAGGCTGAAGCAc                                  >  1:416553/1‑43 (MQ=255) tATTGTTTTAGTCCTGTGCGTTCAGGCGAACAGGCTGAAGCAc                                  >  1:423816/1‑43 (MQ=255) tATTGTTTTAGTCCTGTGCGTTCAGGCGAACAGGCTGAAGCAc                                  >  1:60305/1‑43 (MQ=255) tATTGTTTTAGTCCTGTGCGTTCAGGCGAACAGGCTGAAGCAc                                  >  1:84465/1‑43 (MQ=255) tATTGTTTTAGTCCTGTGCGTTCAGGCGAACAGGCTGAAGCAc                                  >  1:92911/1‑43 (MQ=255) tATTGTTTTAGTCCTGTGCGTTCAGGCGAACAGGCTGAAGCAc                                  >  1:99907/1‑43 (MQ=255)              cTGTGCGTTCAGGCGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAAc  <  1:40972/62‑1 (MQ=255)              cTGTGCGTTCAGGCGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAAc  <  1:84467/62‑1 (MQ=255)                          |                                                  TATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAAGCACTGCCGGAAGCGTATGAACCGATTGAGGTTAAC  >  minE/726310‑726384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑