| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,643,860 | A→G | 100% | R9R (CGT→CGC) | purC ← | phosphoribosylaminoimidazole‑succinocarboxamide synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,643,860 | 0 | A | G | 91.7% | 30.7 / ‑3.2 | 12 | R9R (CGT→CGC) | purC | phosphoribosylaminoimidazole‑succinocarboxamide synthetase |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (11/0); total (11/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.33e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTT > minE/1643804‑1643916 | aaTTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGAt > 1:119149/1‑61 (MQ=255)aaTTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGAt > 1:14008/1‑61 (MQ=255)aaTTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGAt > 1:141575/1‑61 (MQ=255)aaTTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGAt > 1:15754/1‑61 (MQ=255)aaTTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGAt > 1:243927/1‑61 (MQ=255)aaTTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGAt > 1:244679/1‑61 (MQ=255)aaTTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGAt > 1:424577/1‑61 (MQ=255) cACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg > 1:327943/1‑62 (MQ=255) cACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg > 1:348631/1‑62 (MQ=255) tCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTg < 1:40890/62‑1 (MQ=255) tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGtt > 1:378253/1‑61 (MQ=255) tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGtt > 1:389372/1‑61 (MQ=255) | AATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTT > minE/1643804‑1643916 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |