| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,827,842 | Δ1 bp | coding (1085/2562 nt) | mutS → | methyl‑directed mismatch repair protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,827,840 | 0 | C | . | 98.9% | 371.5 / ‑1.8 | 87 | coding (1083/2562 nt) | mutS | methyl‑directed mismatch repair protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (66/20); total (66/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
CAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCG > minE/1827772‑1827897 | cAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCGcca < 1:4194230/71‑2 (MQ=255) tCAGGTCGGCGCCCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:3393077/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCCCGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:1243021/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTGTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:1332194/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCATGCGATCTGGCCCg > 1:2801855/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGccc > 1:2246662/1‑70 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:4068283/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:220693/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:427158/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:2341543/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:2344767/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:2454599/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:24566/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:2484201/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:2681728/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:2725912/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:956119/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:4056709/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:2852261/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:3968071/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:3211552/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:3220918/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:3705581/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:3526693/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:462681/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:1248214/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:1329220/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:1934789/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:1526640/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:1840594/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:770276/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:1687604/1‑71 (MQ=255) tCAGGTAGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:563110/1‑71 (MQ=255) tCGGCGACCTGGAACGTATTCTGGCCCGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATgc < 1:687300/71‑1 (MQ=255) tCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATgc > 1:4322665/1‑71 (MQ=255) tCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATgc > 1:855238/1‑71 (MQ=255) tCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATgc < 1:3099422/71‑1 (MQ=255) tCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATgc > 1:1373907/1‑71 (MQ=255) tCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATgc < 1:1382208/71‑1 (MQ=255) tCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATgc < 1:1618849/71‑1 (MQ=255) tCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATgc > 1:2580477/1‑71 (MQ=255) tCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATgc < 1:675051/71‑1 (MQ=255) tCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATgc < 1:601757/71‑1 (MQ=255) tCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATgc < 1:2079509/71‑1 (MQ=255) tCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATgc < 1:4301479/71‑1 (MQ=255) tCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATg > 1:4259570/1‑70 (MQ=255) tCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATg > 1:3731021/1‑70 (MQ=255) tCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATg > 1:1247464/1‑70 (MQ=255) tCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCGGGCCCGTATgc < 1:741510/71‑1 (MQ=255) tCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTC‑CCCACGCGATCTGGCCCGTATgc < 1:417226/71‑1 (MQ=255) tCGGCGACCTGGAACGTACTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATgc > 1:1505560/1‑71 (MQ=255) cGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCa > 1:48040/1‑71 (MQ=255) cGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCa > 1:1121254/1‑71 (MQ=255) cGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCa > 1:1928643/1‑71 (MQ=255) cGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCa > 1:2103909/1‑71 (MQ=255) tATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGc > 1:981345/1‑70 (MQ=255) cTGGCACGTCTGGCTTTACGAACTTCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACt < 1:2190664/70‑1 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAAc > 1:473200/1‑69 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACt < 1:2063227/70‑1 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACt < 1:1092751/70‑1 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACt < 1:869887/70‑1 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACt < 1:118392/70‑1 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACt < 1:1759026/70‑1 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACt < 1:572573/70‑1 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACt > 1:51422/1‑70 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACt < 1:481312/70‑1 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACt < 1:476148/70‑1 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACt < 1:2810071/70‑1 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTg > 1:2148242/1‑71 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTg > 1:384217/1‑71 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTg > 1:2863938/1‑71 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTg > 1:859791/1‑71 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTg > 1:1847450/1‑71 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTg > 1:1903450/1‑71 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTg > 1:2712583/1‑71 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTg > 1:619351/1‑71 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTg > 1:58731/1‑71 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTg > 1:4179147/1‑71 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTg > 1:2037623/1‑71 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTg > 1:261891/1‑71 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTg > 1:2344300/1‑71 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTg > 1:345324/1‑71 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTg > 1:227165/1‑71 (MQ=255) cTGGCACGTCTGGCTTTACGAACTGCTCG‑CCAAGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTg > 1:2792569/1‑71 (MQ=255) tGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACt < 1:758561/69‑1 (MQ=255) ttACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCtgcgtgcg > 1:1958087/1‑71 (MQ=255) ttACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCtgcgtgcg > 1:4379684/1‑71 (MQ=255) ttACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCtgcgtgc > 1:2884178/1‑70 (MQ=255) | CAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCG > minE/1827772‑1827897 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |