| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,948,809 | 0 | G | A | 43.2% | 19.0 / 56.2 | 44 | N3N (AAC→AAT) | gcvH | glycine cleavage complex lipoylprotein |
| Reads supporting (aligned to +/- strand): ref base G (4/21); new base A (19/0); total (23/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.43e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTT > minE/1948742‑1948879 | tgtAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTg < 1:1453246/71‑1 (MQ=255)tgtAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTg < 1:55459/71‑1 (MQ=255)tgtAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTg < 1:441377/71‑1 (MQ=255) gtAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTg < 1:4313588/70‑1 (MQ=255) tAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCt < 1:1114403/71‑1 (MQ=255) tAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCt < 1:4304432/71‑1 (MQ=255) tAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCt < 1:3763639/71‑1 (MQ=255) tAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCt < 1:3595063/71‑1 (MQ=255) tAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCt < 1:3297079/71‑1 (MQ=255) tACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCaaa > 1:779797/1‑70 (MQ=255) tACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCaaa > 1:442052/1‑70 (MQ=255) tACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCaaa > 1:3348998/1‑70 (MQ=255) tACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCaaa > 1:4197278/1‑70 (MQ=255) aGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTa < 1:3313395/71‑1 (MQ=255) aGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTa < 1:3078255/71‑1 (MQ=255) aGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTa < 1:3060595/71‑1 (MQ=255) aGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTa < 1:4251787/71‑1 (MQ=255) aGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTa < 1:3042648/71‑1 (MQ=255) aGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTa < 1:1289872/71‑1 (MQ=255) aGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTa < 1:1557011/71‑1 (MQ=255) cTTTGCTGTATTTCAGTTCTGCTGGTACATTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGAc > 1:3271006/1‑69 (MQ=255) cTTTGCTGTATTTCAGTTCTGCTGGTACATTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACgg > 1:1826601/1‑71 (MQ=255) cTTTGCTGTATTTCAGTTCTGCTGGTACATTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACgg > 1:4369304/1‑71 (MQ=255) cTTTGCTGTATTTCAGTTCTGCTGGTACATTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACgg > 1:4295614/1‑71 (MQ=255) cTTTGCTGTATTTCAGTTCTGCTGGTACATTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACgg > 1:4289399/1‑71 (MQ=255) cTTTGCTGTATTTCAGTTCTGCTGGTACATTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACgg > 1:4260294/1‑71 (MQ=255) cTTTGCTGTATTTCAGTTCTGCTGGTACATTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACgg > 1:1636946/1‑71 (MQ=255) cTTTGCTGTATTTCAGTTCTGCTGGTACATTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACgg > 1:3930298/1‑71 (MQ=255) cTTTGCTGTATTTCAGTTCTGCTGGTACATTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACgg > 1:875667/1‑71 (MQ=255) cTTTGCTGTATTTCAGTTCTGCTGGTACATTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACgg > 1:2993764/1‑71 (MQ=255) cTTTGCTGTATTTCAGTTCTGCTGGTACATTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACgg > 1:708899/1‑71 (MQ=255) cTTTGCTGTATTTCAGTTCTGCTGGTACATTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACgg > 1:2581953/1‑71 (MQ=255) cTTTGCTGTATTTCAGTTCTGCTGGTACATTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACgg > 1:641518/1‑71 (MQ=255) cTTTGCTGTATTTCAGTTCTGCTGGTACATTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACgg > 1:2721017/1‑71 (MQ=255) cTTTGCTGTATTTCAGTTCTGCTGGTACATTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACgg > 1:3112788/1‑71 (MQ=255) cTTTGCTGTATTTCAGTTCTGCTGGTACATTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACgg > 1:2925516/1‑71 (MQ=255) cTTTGCTGTATTTCAGTTCTGCTGGTACATTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACg > 1:3796552/1‑70 (MQ=255) cTTTGCTGTATTTCAGTTCTGCTGGTACATTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACg > 1:3855369/1‑70 (MQ=255) cTTTGCTGTATTTCAGTTCTGCTGGTACATTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACg > 1:892002/1‑70 (MQ=255) tCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTa < 1:3343071/70‑1 (MQ=255) ttCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTa < 1:275564/42‑1 (MQ=255) ctgGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACaaa < 1:76109/69‑1 (MQ=255) ctgGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACaaa < 1:940203/69‑1 (MQ=255) ctgGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACaaa < 1:155030/69‑1 (MQ=255) gTTGCTCATCTATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGttt < 1:1096727/71‑1 (MQ=255) gTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGttt < 1:3440718/71‑1 (MQ=255) gTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGttt < 1:3205504/71‑1 (MQ=255) gTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGttt < 1:444947/71‑1 (MQ=255) gTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGttt < 1:2891999/71‑1 (MQ=255) gTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGttt < 1:2595646/71‑1 (MQ=255) gTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGttt < 1:2295259/71‑1 (MQ=255) gTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGttt < 1:228578/71‑1 (MQ=255) gTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGttt < 1:2135531/71‑1 (MQ=255) gTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGttt < 1:848993/71‑1 (MQ=255) gTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGttt < 1:851699/71‑1 (MQ=255) gTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGttt < 1:1751513/71‑1 (MQ=255) gTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGttt < 1:885567/71‑1 (MQ=255) gTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGttt < 1:890639/71‑1 (MQ=255) gTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGttt < 1:914950/71‑1 (MQ=255) | TGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTT > minE/1948742‑1948879 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |