| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,132,611 | 1 | . | G | 33.3% | 17.9 / 37.0 | 24 | coding (337/1890 nt) | deaD | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base . (8/8); new base G (0/8); total (8/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.22e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTG‑‑‑GCCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCAACC > minE/2132561‑2132681 | aaCGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTG‑‑‑GCCGCCGTACAGAGCAAcc > 1:3467221/1‑70 (MQ=255)aaCGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTG‑‑‑GCCGCCGTACAGAGCAACca > 1:3218671/1‑71 (MQ=255)aaCGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTG‑‑‑GCCGCCGTACAGAGCAACca > 1:1773043/1‑71 (MQ=255)aaCGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTG‑‑‑GCCGCCGTACAGAGCAACca > 1:428098/1‑71 (MQ=255)aaCGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTG‑‑‑GCCGCCGTACAGAGCAACca > 1:2524998/1‑71 (MQ=255)aaCGAACTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTG‑‑‑GCCGCCGTACAGAGCAACca > 1:3728556/1‑71 (MQ=255) gCAGGGCGCGTAATTGCACGTCATAACGCTG‑‑‑GCCGCCGTACAGAGCAACCACATTTACGCCGCGCATgtgt > 1:2812753/1‑71 (MQ=255) gCAGGGCGCGTAATTGCACGTCATAACGCTG‑‑‑GCCCCCGTACAGAGCAACCACATTTACGCCGCGCATgtgt > 1:1463690/1‑71 (MQ=255) gggCGCGTAATTGCACGTCATAACGCTGGCCGCCGCCGTACAGAGCAACCACATTTACGCCGCGCAt < 1:1003428/67‑1 (MQ=255) gggCGCGTAATTGCACGTCATAACGCTGGCCGCCGCCGTACAGAGCAACCACATTTACGCCGCGCAt < 1:3443032/67‑1 (MQ=255) gggCGCGTAATTGCACGTCATAACGCTGGCCGCCGCCGTACAGAGCAACCACATTTACGCCGCGCAt < 1:3189821/67‑1 (MQ=255) gggCGCGTAATTGCACGTCATAACGCTGGCCGCCGCCGTACAGAGCAACCACATTTACGCCGCGCAt < 1:2418061/67‑1 (MQ=255) gggCGCGTAATTGCACGTCATAACGCTGGCCGCCGCCGTACAGAGCAACCACATTTACGCCGCGCAt < 1:62038/67‑1 (MQ=255) gggCGCGTAATTGCACGTCATAACGCTGGCCGCCGCCGTACAGAGCAACCACATTTACGCCGCGCAt < 1:1654163/67‑1 (MQ=255) ggCGCGTAATTGCACGTCATAACGCTGGCCGCCGCCGTACAGAGCAACCACATTTACGCCGCGCAt < 1:1490038/66‑1 (MQ=255) tcgcgTAATTTCACGTCATAACGCTGGCCGCCGCCGTACAGAGCAACCACATTTACGCCGCGCAt < 1:4342503/64‑1 (MQ=255) aCGTCATAACGCTG‑‑‑GCCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCAt < 1:634550/71‑1 (MQ=255) aCGTCATAACGCTG‑‑‑GCCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCAt < 1:635546/71‑1 (MQ=255) aCGTCATAACGCTG‑‑‑GCCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCAt < 1:2551933/71‑1 (MQ=255) aCGTCATAACGCTG‑‑‑GCCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCAt < 1:2451516/71‑1 (MQ=255) aCGTCATAACGCTG‑‑‑GCCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCAt < 1:1702978/71‑1 (MQ=255) aCGTCATAACGCTG‑‑‑GCCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCAt < 1:1491678/71‑1 (MQ=255) aCGTCATAACGCTG‑‑‑GCCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCAt < 1:1268574/71‑1 (MQ=255) cGTCATAACGCTG‑‑‑GCCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCAt < 1:4078052/70‑1 (MQ=255) g‑‑‑gCCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCAAcc > 1:3498279/1‑71 (MQ=255) | AACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTG‑‑‑GCCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCAACC > minE/2132561‑2132681 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |