Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,428,2160CT72.3% 250.9 / 117.4 148V89I (GTC→ATC) rffEUDP‑N‑acetyl glucosamine‑2‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (11/30);  new base T (57/50);  total (68/80)
Fisher's exact test for biased strand distribution p-value = 5.39e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 152 total aligned reads displayed.

CTGATAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATCTCTGTCAGGC  >  minE/2428154‑2428284
                                                              |                                                                    
cTGATAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGtt                                                              >  1:2865179/1‑71 (MQ=255)
        aCGCCGCCAGGCTGGTTGCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCg                                                      >  1:1839184/1‑71 (MQ=255)
        aCGCCGCCAGGCTGGTTGCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCg                                                      >  1:726053/1‑71 (MQ=255)
        aCGCCGCCAGGCTGGTTGCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCg                                                      >  1:2277535/1‑71 (MQ=255)
        aCGCCGCCAGGCTGGTTGCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCg                                                      >  1:1760636/1‑71 (MQ=255)
        aCGCCGCCAGGCTGGTTGCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCg                                                      >  1:2321444/1‑71 (MQ=255)
        aCGCCGCCAGGCTGGTTGCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCg                                                      >  1:1114485/1‑71 (MQ=255)
        aCGCCGCCAGGCTGGTTGCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCg                                                      >  1:4225788/1‑71 (MQ=255)
          gccgccAGGCTGGTTGCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGc                                                    <  1:3653427/71‑1 (MQ=255)
          gccgccAGGCTGGTTGCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGc                                                    <  1:1555815/71‑1 (MQ=255)
          gccgccAGGCTGGTTGCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGc                                                    <  1:2397415/71‑1 (MQ=255)
                  gCTGGTTGCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAg                                            <  1:3744649/71‑1 (MQ=255)
                  gCTGGTTGCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAg                                            <  1:3353901/71‑1 (MQ=255)
                  gCTGGTTGCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAg                                            <  1:3830335/71‑1 (MQ=255)
                  gCTGGTTGCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAg                                            <  1:63570/71‑1 (MQ=255)
                  gCTGGTTGCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAg                                            <  1:3854361/71‑1 (MQ=255)
                  gCTGGTTGCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAg                                            <  1:4064243/71‑1 (MQ=255)
                  gCTGGTTGCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAg                                            <  1:2071991/71‑1 (MQ=255)
                  gCTGGTTGCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAg                                            <  1:2721314/71‑1 (MQ=255)
                   cTGGTTGCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAgg                                           >  1:4002765/1‑71 (MQ=255)
                         gCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAg                                     >  1:892453/1‑71 (MQ=255)
                         gCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAg                                     >  1:2783368/1‑71 (MQ=255)
                         gCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAg                                     >  1:3838653/1‑71 (MQ=255)
                          ccAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGc                                    <  1:1188379/71‑1 (MQ=255)
                          ccAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGc                                    <  1:3417554/71‑1 (MQ=255)
                          ccAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGc                                    <  1:3520192/71‑1 (MQ=255)
                          ccAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGc                                    <  1:229540/71‑1 (MQ=255)
                          ccAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGtttatc                                     <  1:744045/70‑4 (MQ=255)
                          ccAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGc                                    <  1:1840076/71‑1 (MQ=255)
                          ccAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGc                                    <  1:443478/71‑1 (MQ=255)
                          ccAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGc                                    <  1:671109/71‑1 (MQ=255)
                          ccAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGc                                    <  1:170635/71‑1 (MQ=255)
                             gCGTCGTCGTCGTATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCt                                 <  1:3675681/71‑1 (MQ=255)
                             gCGTCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCt                                 <  1:48767/71‑1 (MQ=255)
                                  gtcgtcgtATCGCCGTGATCCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTc                               >  1:2192424/1‑68 (MQ=255)
                                  gtcgtcgtATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTc                               >  1:1705568/1‑68 (MQ=255)
                                  gtcgtcgtATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTc                               >  1:411875/1‑68 (MQ=255)
                                  gtcgtcgtATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTc                               >  1:1119729/1‑68 (MQ=255)
                                  gtcgtcgtATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTc                               >  1:2525918/1‑68 (MQ=255)
                                     gtcgtATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGaa                          <  1:3488352/70‑1 (MQ=255)
                                     gtcgtATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGaa                          <  1:271726/70‑1 (MQ=255)
                                     gtcgtATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGaa                          <  1:2790112/70‑1 (MQ=255)
                                     gtcgtATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGaa                          <  1:1412387/70‑1 (MQ=255)
                                       cgtATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATcc                       >  1:3956761/1‑71 (MQ=255)
                                        gtATCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATcc                       <  1:2859656/70‑1 (MQ=255)
                                        gtATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATcc                       <  1:2264214/70‑1 (MQ=255)
                                        gtATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATcc                       <  1:2937024/70‑1 (MQ=255)
                                           tCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGc                                    <  1:571891/54‑1 (MQ=255)
                                           tCGCCGTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGc                                    <  1:2435316/54‑1 (MQ=255)
                                               cGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATcc                       >  1:1237170/1‑63 (MQ=255)
                                               cGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATcc                       >  1:3230414/1‑63 (MQ=255)
                                               cGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATcc                       >  1:488730/1‑63 (MQ=255)
                                               cGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATcc                       >  1:297734/1‑63 (MQ=255)
                                               cGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATcc                       >  1:2054094/1‑63 (MQ=255)
                                                gTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGaa                          >  1:1985781/1‑59 (MQ=255)
                                                gTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTa              <  1:19402/71‑1 (MQ=255)
                                                gTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTa              >  1:1011008/1‑71 (MQ=255)
                                                gTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTa              >  1:4225516/1‑71 (MQ=255)
                                                gTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTa              <  1:1971577/71‑1 (MQ=255)
                                                gTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTa              >  1:4269123/1‑71 (MQ=255)
                                                gTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTa              >  1:3989584/1‑71 (MQ=255)
                                                gTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTa              <  1:4354750/71‑1 (MQ=255)
                                                gTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTa              <  1:763959/71‑1 (MQ=255)
                                                gTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTa              <  1:1218655/71‑1 (MQ=255)
                                                gTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTa              <  1:3798762/71‑1 (MQ=255)
                                                gTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTa              <  1:3726150/71‑1 (MQ=255)
                                                gTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTa              <  1:367646/71‑1 (MQ=255)
                                                gTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTa              >  1:772975/1‑71 (MQ=255)
                                                gTGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTa              >  1:33727/1‑71 (MQ=255)
                                                 tGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTa              <  1:3759801/70‑1 (MQ=255)
                                                 tGAACCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTa              <  1:661847/70‑1 (MQ=255)
                                                   aaCCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGc                                    <  1:1780947/46‑1 (MQ=255)
                                                   aaCCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTAtc            <  1:3747020/70‑1 (MQ=255)
                                                   aaCCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTAtc            <  1:3722842/70‑1 (MQ=255)
                                                   aaCCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTAtc            <  1:3337602/70‑1 (MQ=255)
                                                   aaCCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTAtc            <  1:3896208/70‑1 (MQ=255)
                                                   aaCCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTAtc            <  1:2500998/70‑1 (MQ=255)
                                                   aaCCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTAtc            <  1:3336817/70‑1 (MQ=255)
                                                   aaCCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTAtc            <  1:2253979/70‑1 (MQ=255)
                                                    aCCAGCACTATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTAtctc          <  1:1256634/71‑1 (MQ=255)
                                                    aCCAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTAtctc          <  1:1560050/71‑1 (MQ=255)
                                                     ccAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGaa                          >  1:4178917/1‑54 (MQ=255)
                                                     ccAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATctct         >  1:3910022/1‑71 (MQ=255)
                                                     ccAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATctct         >  1:2273316/1‑71 (MQ=255)
                                                     ccAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATctct         <  1:3214366/71‑1 (MQ=255)
                                                     ccAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATctct         <  1:4071576/71‑1 (MQ=255)
                                                     ccAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATctct         <  1:3959677/71‑1 (MQ=255)
                                                     ccAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATctct         <  1:322117/71‑1 (MQ=255)
                                                     ccAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATctct         <  1:2664739/71‑1 (MQ=255)
                                                     ccAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATctct         <  1:1822600/71‑1 (MQ=255)
                                                     ccAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATctct         <  1:181982/71‑1 (MQ=255)
                                                     ccAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATctct         <  1:1394496/71‑1 (MQ=255)
                                                      cAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATCTCTg        >  1:4239728/1‑71 (MQ=255)
                                                      cAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATCTCTg        >  1:3065946/1‑71 (MQ=255)
                                                      cAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATCTCTg        >  1:204282/1‑71 (MQ=255)
                                                      cAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATCTCTg        >  1:198786/1‑71 (MQ=255)
                                                      cAGCACGATGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATCTCTg        >  1:1029034/1‑71 (MQ=255)
                                                      cAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATctct         <  1:2659485/70‑1 (MQ=255)
                                                            gacgTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATCTCTGTCAGGc  <  1:2174076/71‑1 (MQ=255)
                                                             acgTCTGGTTTGATCTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATctct         <  1:3238111/63‑1 (MQ=255)
                                                              |                                                                    
CTGATAAAACGCCGCCAGGCTGGTTGCCAGCGTCGTCGTCGTATCGCCGTGAACCAGCACGACGTCTGGTTTGAACTCGGCAAGAATAGGTTTTAGCCCTTCCAGAATCCGACAGGTTATCTCTGTCAGGC  >  minE/2428154‑2428284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑