| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,600,278 | 1 | . | C | 39.5% | 7.2 / 73.3 | 38 | intergenic (‑131/‑72) | yhiR/prlC | predicted DNA (exogenous) processing protein/oligopeptidase A |
| Reads supporting (aligned to +/- strand): ref base . (18/5); new base C (15/0); total (33/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGTATACCGAAAAGAGGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑CCCCATGCTACACACATTATGTAAAGCGCCTGTTGAGCGCTTCCTTAACCTCTTTAACCAGGAC > minE/2600212‑2600342 | cAGTATACCGAAAAGAGGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTaa > 1:680934/1‑67 (MQ=255)cAGTATACCGAAAAGAGGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑ccc > 1:567378/1‑70 (MQ=255)cAGTATACCGAAAAGAGGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑ccc > 1:2180606/1‑70 (MQ=255)cAGTATACCGAAAAGAGGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑ccc > 1:2146010/1‑70 (MQ=255)cAGTATACCGAAAAGAGGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑ccc > 1:1965591/1‑70 (MQ=255)cAGTATACCGAAAAGAGGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑ccc > 1:3741148/1‑70 (MQ=255)cAGTATACCGAAAAGAGGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑ccc > 1:4220197/1‑70 (MQ=255)cAGTATACCGAAAAGAGGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑ccc > 1:1843476/1‑70 (MQ=255)cAGTATACCGAAAAGAGGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑ccc > 1:3712564/1‑70 (MQ=255)cAGTATACCGAAAAGAGGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑ccc > 1:1444789/1‑70 (MQ=255)cAGTATACCGAAAAGAGGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑ccc > 1:1341852/1‑70 (MQ=255)cAGTATACCGAAAAGAGGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑ccc > 1:1224621/1‑70 (MQ=255) cGAAAAGAGGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑CCCCATGCTaca < 1:755859/71‑1 (MQ=255) cGAAAAGAGGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑CCCCATGCTaca < 1:4078383/71‑1 (MQ=255) agagGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑CCCCATGCTACACACAt < 1:724555/71‑1 (MQ=255) gagGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAACCCCCATGCTAcacaca > 1:3331353/1‑70 (MQ=255) gagGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAACCCCCATGCTACACACAt > 1:413629/1‑71 (MQ=255) gagGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAACCCCCATGCTACACACAt > 1:376615/1‑71 (MQ=255) gagGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAACCCCCATGCTACACACAt > 1:3885299/1‑71 (MQ=255) gagGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAACCCCCATGCTACACACAt > 1:3037795/1‑71 (MQ=255) gagGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAACCCCCATGCTACACACAt > 1:3936306/1‑71 (MQ=255) gagGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAACCCCCATGCTACACACAt > 1:4051494/1‑71 (MQ=255) gagGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAACCCCCATGCTACACACAt > 1:1009033/1‑71 (MQ=255) gagGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAACCCCCATGCTACACACAt > 1:706524/1‑71 (MQ=255) gagGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAACCCCCATGCTACACACAt > 1:904162/1‑71 (MQ=255) gagGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAACCCCCATGCTACACACAt > 1:960206/1‑71 (MQ=255) gagGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAACCCCCATGCTACACACAt > 1:1975378/1‑71 (MQ=255) gagGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAACCCCCATGCTACACACAt > 1:1523723/1‑71 (MQ=255) gagGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAACCCCCATGCTACACACAt > 1:2756885/1‑71 (MQ=255) gagGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAACCCCCATGCTACACACAt > 1:1755627/1‑71 (MQ=255) gagGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑c > 1:1121217/1‑54 (MQ=255) agGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑CCCCATGCTACACACATTa > 1:1861395/1‑71 (MQ=255) agGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑CCCCATGCTACACACATTa > 1:1950545/1‑71 (MQ=255) agGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑CCCCATGCTACACACATTa > 1:495549/1‑71 (MQ=255) agGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑CCCCATGCTACACACATTa > 1:1784470/1‑71 (MQ=255) agGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑CCCCATGCTACACACATTa > 1:1998896/1‑71 (MQ=255) agGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑CCCCATGCTACACACATTa > 1:3914637/1‑71 (MQ=255) agGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑CCCCATGCTACACACATTa > 1:2657786/1‑71 (MQ=255) agGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑CCCCATGCTACACACATTa > 1:3686593/1‑71 (MQ=255) agGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑CCCCATGCTACACACATTa > 1:1200947/1‑71 (MQ=255) agGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑CCCCATGCTACACACATTa > 1:3033204/1‑71 (MQ=255) agGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑CCCCATGCTACACACATTa > 1:3209222/1‑71 (MQ=255) gccgccgccGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑CCCCATGCTACACACATTATg > 1:1002530/1‑71 (MQ=255) gccgccgccGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑CCCCATGCTACACACATTATg > 1:3961449/1‑71 (MQ=255) tttCTCATTGAAATTCACTACACTTAA‑CCCCATGCTACACACATTATGTAAAGCGCCTGTTGAGCGCTTcc > 1:2846188/1‑71 (MQ=255) tttCTCATTGAAATTCACTACACTTAA‑CCCCATGCTACACACATTATGTAAAGCGCCTGTTGAGCGCTTcc > 1:1508478/1‑71 (MQ=255) tttCTCATTGAAATTCACTACACTTAA‑CCCCATGCTACACACATTATGTAAAGCGCCTGTTGAGCGCTTcc > 1:2851346/1‑71 (MQ=255) gAAATTCACTACACTTAA‑CCCCATGCTACACACATTATGTAAAGCGcc > 1:3375912/1‑48 (MQ=255) gAAATTCACTACACTTAA‑CCCCATGCTACACACATTATGTAAAGCGCCTGTTGAGCGCTTCCTTAACctct > 1:3381044/1‑71 (MQ=255) cacTTAA‑CCCCATGCTACACACATTATGTAAAGCGCCTGTTGAGCGCTTCCTTAACCTCTTTAACCAGGAc < 1:1978718/71‑1 (MQ=255) cacTTAA‑CCCCATGCTACACACATTATGTAAAGCGCCTGTTGAGCGCTTCCTTAACCTCTTTAACCAGGAc < 1:27343/71‑1 (MQ=255) | CAGTATACCGAAAAGAGGCCGCCGCCGCGAAAGCGTAACGTTTCTCATTGAAATTCACTACACTTAA‑CCCCATGCTACACACATTATGTAAAGCGCCTGTTGAGCGCTTCCTTAACCTCTTTAACCAGGAC > minE/2600212‑2600342 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |