Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE483,9061.C31.8% 47.5 / 67.4 44coding (1110/1266 nt)tolAmembrane anchored protein in TolA‑TolQ‑TolR complex
Reads supporting (aligned to +/- strand):  ref base . (8/22);  new base C (14/0);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 5.56e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTT  >  minE/483848‑483971
                                                           |                                                                 
agtTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATg                                                       >  1:1091816/1‑71 (MQ=255)
agtTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATg                                                       >  1:2596978/1‑71 (MQ=255)
agtTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATg                                                       >  1:3522257/1‑71 (MQ=255)
agtTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATg                                                       >  1:1177370/1‑71 (MQ=255)
       tGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTg                                                 <  1:1016986/70‑1 (MQ=255)
                            cAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGc                            >  1:3701757/1‑70 (MQ=255)
                            cAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGc                            >  1:3629681/1‑70 (MQ=255)
                            cAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGc                            >  1:2388058/1‑70 (MQ=255)
                            cAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGc                            >  1:2451902/1‑70 (MQ=255)
                             aaaaCCTGTACGCTGCGCATAAACCTGGCACCCCGATGGTATGTTACTGGATATCaaa                                        >  1:2822867/1‑58 (MQ=255)
                             aaaaCCTGTACGCTGCGCATAAAACTGGCACCCCGATGGTATGTTACTGGATATCaaa                                        >  1:4286115/1‑58 (MQ=255)
                             aaaaCCTGTACGCTGCGCATAAAACTGGCACCCCGATGGTATGTTACTGGATATCaaa                                        >  1:3754122/1‑58 (MQ=255)
                             aaaaCCTGTACGCTGCGCATAAAACTGGCACCCCGATGGTATGTTACTGGATATCaaa                                        >  1:3719268/1‑58 (MQ=255)
                             aaaaCCTGTACGCTGCGCATAAAACTGGCACCCCGATGGTATGTTACTGGATATCaaa                                        >  1:3099840/1‑58 (MQ=255)
                             aaaaCCTGTACGCTGCGCATAAAACTGGCACCCCGATGGTATGTTACTGGATATCaaa                                        >  1:2944919/1‑58 (MQ=255)
                             aaaaCCTGTACGCTGCGCATAAAACTGGCACCCCGATGGTATGTTACTGGATATCaaa                                        >  1:865914/1‑58 (MQ=255)
                             aaaaCCTGTACGCTGCGCATAAAACTGGCACCCCGATGGTATGTTACTGGATATCaaa                                        >  1:2733265/1‑58 (MQ=255)
                             aaaaCCTGTACGCTGCGCATAAAACTGGCACCCCGATGGTATGTTACTGGATATCaaa                                        >  1:694261/1‑58 (MQ=255)
                             aaaaCCTGTACGCTGCGCATAAAACTGGCACCCCGATGGTATGTTACTGGATATCaaa                                        >  1:87237/1‑58 (MQ=255)
                             aaaaCCTGTACGCTGCGCATAAAACTGGCACCCCGATGGTATGTTACTGGATATCaaa                                        >  1:2256810/1‑58 (MQ=255)
                             aaaaCCTGTACGCTGCGCATAAAACTGGCACCCCGATGGTATGTTACTGGATATCaaa                                        >  1:905459/1‑58 (MQ=255)
                             aaaaCCTGTACGCTGCGCATAAAACTGGCACCCCGATGGTATGTTACTGGATATCaaa                                        >  1:1210582/1‑58 (MQ=255)
                             aaaaCCTGTACGCTGCGCATAAAACTGGCACCCCGATGGTATGTTACTGGATATCaaa                                        >  1:2699151/1‑58 (MQ=255)
                                                tAAAACTGGCACCCGATGGTATGTTACTGGATATCa                                          <  1:3369578/36‑1 (MQ=255)
                                                tAAAACTGGCACCCGATGGTATGTTACTGGATATCa                                          <  1:4339857/36‑1 (MQ=255)
                                                tAAAACTGGCACCCGATGGTATGTTACTGGATATCa                                          <  1:4323634/36‑1 (MQ=255)
                                                tAAAACTGGCACCCGATGGTATGTTACTGGATATCa                                          <  1:1185373/36‑1 (MQ=255)
                                                tAAAACTGGCACCCGATGGTATGTTACTGGATATCa                                          <  1:1197152/36‑1 (MQ=255)
                                                tAAAACTGGCACCCGATGGTATGTTACTGGATATCa                                          <  1:1229977/36‑1 (MQ=255)
                                                tAAAACTGGCACCCGATGGTATGTTACTGGATATCa                                          <  1:1516503/36‑1 (MQ=255)
                                                tAAAACTGGCACCCGATGGTATGTTACTGGATATCa                                          <  1:3140474/36‑1 (MQ=255)
                                                tAAAACTGGCACCCGATGGTATGTTACTGGATATCa                                          <  1:1684052/36‑1 (MQ=255)
                                                tAAAACTGGCACCCGATGGTATGTTACTGGATATCa                                          <  1:2246020/36‑1 (MQ=255)
                                                tAAAACTGGCACCCGATGGTATGTTACTGGATATCa                                          <  1:2795428/36‑1 (MQ=255)
                                                tAAAACTGGCACCCGATGGTATGTTACTGGATATCa                                          <  1:2439878/36‑1 (MQ=255)
                                                     cTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGtt  <  1:993415/71‑1 (MQ=255)
                                                     cTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGtt  <  1:947498/71‑1 (MQ=255)
                                                     cTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGtt  <  1:555097/71‑1 (MQ=255)
                                                     cTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGtt  <  1:537409/71‑1 (MQ=255)
                                                     cTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGtt  <  1:2812897/71‑1 (MQ=255)
                                                     cTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGtt  <  1:2713812/71‑1 (MQ=255)
                                                     cTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGtt  <  1:1627285/71‑1 (MQ=255)
                                                     cTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGtt  <  1:15673/71‑1 (MQ=255)
                                                     cTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGtt  <  1:1158437/71‑1 (MQ=255)
                                                           |                                                                 
AGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTT  >  minE/483848‑483971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: