| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 526,369 | 0 | G | T | 31.6% | 73.3 / 27.6 | 38 | V11L (GTA→TTA) | ybhL | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (25/1); new base T (12/0); total (37/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGG > minE/526308‑526419 | aaGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAcccc > 1:2049502/1‑70 (MQ=255)aaGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAAcccc > 1:2260766/1‑71 (MQ=255) atTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCTTACAACCCCGggct > 1:858224/1‑71 (MQ=255) atTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCTTACAACCCCGggct > 1:821163/1‑71 (MQ=255) atTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCTTACAACCCCGggct > 1:621425/1‑71 (MQ=255) atTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCTTACAACCCCGggct > 1:1818498/1‑71 (MQ=255) atTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCTTACAACCCCGggct > 1:1854672/1‑71 (MQ=255) atTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCTTACAACCCCGggct > 1:511285/1‑71 (MQ=255) atTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCTTACAACCCCGggct > 1:351566/1‑71 (MQ=255) atTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCTTACAACCCCGggct > 1:3393552/1‑71 (MQ=255) atTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCTTACAACCCCGggct > 1:3301609/1‑71 (MQ=255) atTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCTTACAACCCCGggct > 1:29998/1‑71 (MQ=255) atTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCTTACAACCCCGggct > 1:2797949/1‑71 (MQ=255) atTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCTTACAACCCCGggct > 1:2723295/1‑71 (MQ=255) tataTCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTg > 1:1249927/1‑71 (MQ=255) tataTCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTg > 1:4078832/1‑71 (MQ=255) tataTCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTg > 1:2482279/1‑71 (MQ=255) tataTCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTg > 1:2031171/1‑71 (MQ=255) aTCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGc > 1:1257273/1‑71 (MQ=255) aTCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGc > 1:1311418/1‑71 (MQ=255) aTCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGc > 1:2653207/1‑71 (MQ=255) aTCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGc > 1:3578650/1‑71 (MQ=255) cGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGc > 1:738118/1‑69 (MQ=255) cGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCt > 1:3748763/1‑70 (MQ=255) cGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCt > 1:672245/1‑70 (MQ=255) cGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCt > 1:206083/1‑70 (MQ=255) cGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCt > 1:383983/1‑70 (MQ=255) cGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCt > 1:2082509/1‑70 (MQ=255) cGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCt > 1:2434381/1‑70 (MQ=255) cGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCt > 1:2448275/1‑70 (MQ=255) cGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCt > 1:3138808/1‑70 (MQ=255) cATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAg > 1:3566040/1‑71 (MQ=255) cATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAg > 1:346119/1‑71 (MQ=255) cATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAg > 1:3413504/1‑71 (MQ=255) cATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAg > 1:724649/1‑71 (MQ=255) cATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAg > 1:2472316/1‑71 (MQ=255) cATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAg > 1:1020472/1‑71 (MQ=255) cccACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTgg < 1:3796829/70‑1 (MQ=255) | AAGATATTCCTTATATCTTCAGGAGATCGTCATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGG > minE/526308‑526419 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |