| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 705,870 | 0 | A | G | 63.2% | 5.9 / 15.6 | 19 | intergenic (‑367/+128) | ymcC/serX | predicted outer membrane lipoprotein/tRNA‑Ser |
| Reads supporting (aligned to +/- strand): ref base A (3/4); new base G (11/1); total (14/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.79e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCC > minE/705845‑705938 | cgctcgcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAAc < 1:3228108/70‑1 (MQ=33) cgcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCgg > 1:3411158/1‑71 (MQ=34) cGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGc < 1:1634122/71‑1 (MQ=21) cGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCggg < 1:1528396/69‑1 (MQ=35) cGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCggg < 1:3186504/69‑1 (MQ=35) cGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCggg < 1:2910527/69‑1 (MQ=35) ggCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCgggg > 1:3701467/1‑68 (MQ=25) ggCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGcgt > 1:2843508/1‑69 (MQ=255) ggCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGc > 1:4085411/1‑69 (MQ=25) ggCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCtt > 1:930751/1‑71 (MQ=21) ggCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCt > 1:2853247/1‑70 (MQ=25) ggCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCt > 1:2675343/1‑70 (MQ=25) ggCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCt > 1:231175/1‑70 (MQ=25) ggCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCt > 1:1666663/1‑70 (MQ=25) ggCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCt > 1:3357844/1‑70 (MQ=25) ggCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCt > 1:1100111/1‑70 (MQ=25) ggCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCt > 1:4019306/1‑70 (MQ=25) cACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAAc > 1:2753117/1‑46 (MQ=35) cACTGCGTTCACGGTCTGTCCAACTGGCTGGCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATccccc > 1:3926362/1‑70 (MQ=255) | CGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCC > minE/705845‑705938 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |