BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	52,517	T→C	E757E (GAA→GAG)	imp ←	exported protein required for envelope biosynthesis and integrity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	52,517	0	T	C	100.0%	409.5 / NA	105	E757E (GAA→GAG)	imp	exported protein required for envelope biosynthesis and integrity
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (82/23); total (82/23)

Only 100 of 105 total aligned reads displayed.

GCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGTTCAGCT  >  minE/52453‑52584

gCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                                  >  1:2636858/1‑68 (MQ=255)

gCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATg                                                               >  1:2484132/1‑71 (MQ=255)

gCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATg                                                               >  1:2036278/1‑71 (MQ=255)

gCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATg                                                               >  1:2902578/1‑71 (MQ=255)

gCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATg                                                               >  1:2008290/1‑71 (MQ=255)

gCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATg                                                               >  1:1920994/1‑71 (MQ=255)

gCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATg                                                               >  1:316848/1‑71 (MQ=255)

gCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATg                                                               >  1:1819585/1‑71 (MQ=255)

gCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATg                                                               >  1:1796638/1‑71 (MQ=255)

gCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATg                                                               >  1:3190199/1‑71 (MQ=255)

gCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATg                                                               >  1:1691682/1‑71 (MQ=255)

gCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATg                                                               >  1:2560959/1‑71 (MQ=255)

gCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATg                                                               >  1:3774981/1‑71 (MQ=255)

gCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATg                                                               >  1:3940690/1‑71 (MQ=255)

gCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATg                                                               >  1:4174357/1‑71 (MQ=255)

gCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATg                                                               >  1:4269795/1‑71 (MQ=255)

gCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATg                                                               >  1:843162/1‑71 (MQ=255)

gCAGAATGTTCGAACGCAGCATCTCTTGCGTACACAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATg                                                               >  1:1466112/1‑71 (MQ=255)

      tGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                                   >  1:2273483/1‑61 (MQ=255)

      tGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                                   >  1:2432868/1‑61 (MQ=255)

      tGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                                   >  1:236741/1‑61 (MQ=255)

      tGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                                   >  1:2281454/1‑61 (MQ=255)

      tGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                                   >  1:2642995/1‑61 (MQ=255)

      tGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                                   >  1:1236148/1‑61 (MQ=255)

      tGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                                   >  1:3188498/1‑61 (MQ=255)

      tGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                                   >  1:3244922/1‑61 (MQ=255)

      tGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                                   >  1:335101/1‑61 (MQ=255)

      tGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                                   >  1:3807450/1‑61 (MQ=255)

      tGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                                   >  1:445996/1‑61 (MQ=255)

      tGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                                   >  1:803508/1‑61 (MQ=255)

      tGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                                   >  1:803926/1‑61 (MQ=255)

      tGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                                   >  1:2048746/1‑61 (MQ=255)

      tGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                                   >  1:2043883/1‑61 (MQ=255)

      tGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                                   >  1:201269/1‑61 (MQ=255)

        ttCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                                   >  1:2230527/1‑59 (MQ=255)

        ttCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                                   >  1:4336085/1‑59 (MQ=255)

        ttCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                                   >  1:1279165/1‑59 (MQ=255)

        ttCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                                   >  1:2324740/1‑59 (MQ=255)

        ttCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                                   >  1:2283215/1‑59 (MQ=255)

        ttCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                                   >  1:1253097/1‑59 (MQ=255)

        ttCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGcc                                                       >  1:2365162/1‑71 (MQ=255)

        ttCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGcc                                                       >  1:818835/1‑71 (MQ=255)

        ttCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGcc                                                       >  1:580633/1‑71 (MQ=255)

        ttCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGcc                                                       >  1:1632960/1‑71 (MQ=255)

        ttCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGcc                                                       >  1:1439773/1‑71 (MQ=255)

        ttCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGcc                                                       >  1:403803/1‑71 (MQ=255)

        ttCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGc                                                        >  1:1601469/1‑70 (MQ=255)

        ttCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGc                                                        >  1:2754569/1‑70 (MQ=255)

        ttCGAACGCAGCATCTCTGGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGcc                                                       >  1:4128215/1‑71 (MQ=255)

                cagcaTCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGt                                               <  1:2280602/71‑1 (MQ=255)

                cagcaTCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGt                                               <  1:3098277/71‑1 (MQ=255)

                                               gAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCTTTAt                 <  1:1456911/70‑1 (MQ=255)

                                               gAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTAt                 <  1:2165522/70‑1 (MQ=255)

                                                       gcagcgAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGt        >  1:141583/4‑71 (MQ=255)

                                                       gCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCg         >  1:3517321/1‑70 (MQ=255)

                                                       gCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGt        >  1:2231611/1‑71 (MQ=255)

                                                       gCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGt        >  1:1446192/1‑71 (MQ=255)

                                                       gCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGt        >  1:4030025/1‑71 (MQ=255)

                                                       gCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGt        >  1:3833834/1‑71 (MQ=255)

                                                       gCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGt        >  1:660797/1‑71 (MQ=255)

                                                       gCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGt        >  1:3715968/1‑71 (MQ=255)

                                                       gCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGt        >  1:3715170/1‑71 (MQ=255)

                                                       gCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGt        >  1:3648693/1‑71 (MQ=255)

                                                       gCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGt        >  1:2978970/1‑71 (MQ=255)

                                                        ccGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGt                    >  1:3705556/1‑58 (MQ=255)

                                                        ccGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGtt       >  1:2195422/1‑71 (MQ=255)

                                                        ccGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGtt       >  1:3163186/1‑71 (MQ=255)

                                                        ccGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGtt       >  1:2630617/1‑71 (MQ=255)

                                                        ccGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGtt       >  1:4326827/1‑71 (MQ=255)

                                                        ccGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGtt       >  1:548576/1‑71 (MQ=255)

                                                        ccGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGtt       >  1:605427/1‑71 (MQ=255)

                                                        ccGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGtt       >  1:2829077/1‑71 (MQ=255)

                                                        ccGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGtt       >  1:6785/1‑71 (MQ=255)

                                                        ccGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGtt       >  1:348645/1‑71 (MQ=255)

                                                        ccGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGtt       >  1:307567/1‑71 (MQ=255)

                                                        ccGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGtt       >  1:1649608/1‑71 (MQ=255)

                                                        ccGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGtt       >  1:1314985/1‑71 (MQ=255)

                                                        ccGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGtt       >  1:1765671/1‑71 (MQ=255)

                                                        ccGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGt        >  1:2733723/1‑70 (MQ=255)

                                                         cgcgAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGtt       >  1:3001540/1‑70 (MQ=255)

                                                         cgcgAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGtt       >  1:340774/1‑70 (MQ=255)

                                                         cgcgAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTATATCGTTATCCCAACCg         >  1:273303/1‑68 (MQ=255)

                                                             aaGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCCACCGTTCAGCt  <  1:4246029/71‑1 (MQ=255)

                                                             aaGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGTTCAGCt  <  1:2809763/71‑1 (MQ=255)

                                                             aaGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGTTCAGCt  <  1:1825311/71‑1 (MQ=255)

                                                             aaGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGTTCAGCt  <  1:2168736/71‑1 (MQ=255)

                                                             aaGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGTTCAGCt  <  1:214526/71‑1 (MQ=255)

                                                             aaGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGTTCAGCt  <  1:1746528/71‑1 (MQ=255)

                                                             aaGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGTTCAGCt  <  1:2453880/71‑1 (MQ=255)

                                                             aaGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGTTCAGCt  <  1:3758078/71‑1 (MQ=255)

                                                             aaGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGTTCAGCt  <  1:2130182/71‑1 (MQ=255)

                                                             aaGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGTTCAGCt  <  1:278956/71‑1 (MQ=255)

                                                             aaGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGTTCAGCt  <  1:2511676/71‑1 (MQ=255)

                                                             aaGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGTTCAGCt  <  1:2544758/71‑1 (MQ=255)

                                                             aaGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGTTCAGCt  <  1:1570023/71‑1 (MQ=255)

                                                             aaGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGTTCAGCt  <  1:2070167/71‑1 (MQ=255)

                                                             aaGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGTTCAGCt  <  1:4195882/71‑1 (MQ=255)

                                                             aaGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGTTCAGCt  <  1:256652/71‑1 (MQ=255)

                                                             aaGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGTTCAGCt  <  1:2593458/71‑1 (MQ=255)

                                                             aaGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCCCATGTTGTTTATCGTTATCCCAACCGTTCAGCt  <  1:3056058/71‑1 (MQ=255)

GCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCCCAACCGTTCAGCT  >  minE/52453‑52584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`