Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,081,858	T→C	N104S (AAC→AGC)	ydhY ←	predicted 4Fe‑4S ferridoxin‑type protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,081,858	0	T	C	100.0%	373.7 / NA	96	N104S (AAC→AGC)	ydhY	predicted 4Fe‑4S ferridoxin‑type protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (63/33);  total (63/33)

ACGGCAGGTGTCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTAC  >  minE/1081797‑1081913                                                              |                                                        aCGGCAGGTGTCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCa                                                 <  1:1214744/70‑1 (MQ=255) aCGGCAGGTGTCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCa                                                 <  1:3346765/70‑1 (MQ=255) aCGGCAGGTGTCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCa                                                 <  1:3550063/70‑1 (MQ=255)         tgtCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaataa                                        >  1:724473/1‑71 (MQ=255)         tgtCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaataa                                        >  1:301698/1‑71 (MQ=255)         tgtCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaataa                                        >  1:2464998/1‑71 (MQ=255)         tgtCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaataa                                        >  1:1918371/1‑71 (MQ=255)         tgtCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaataa                                        <  1:1495874/71‑1 (MQ=255)          gtacgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaataat                                       >  1:3507881/4‑71 (MQ=255)          gtCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaataat                                       >  1:1887555/1‑71 (MQ=255)          gtCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaataat                                       >  1:2924727/1‑71 (MQ=255)          gtCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaataat                                       >  1:2902224/1‑71 (MQ=255)          gtCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaataat                                       >  1:2349924/1‑71 (MQ=255)          gtCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaataat                                       >  1:2211582/1‑71 (MQ=255)          gtCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaataat                                       >  1:3539282/1‑71 (MQ=255)          gtCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaataat                                       >  1:1793292/1‑71 (MQ=255)          gtCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaataat                                       >  1:547740/1‑71 (MQ=255)          gtCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaataat                                       >  1:1476875/1‑71 (MQ=255)          gtCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaataat                                       >  1:3853103/1‑71 (MQ=255)          gtCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaataa                                        >  1:1552098/1‑70 (MQ=255)             cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                                    >  1:998307/1‑71 (MQ=255)             cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                                    >  1:1799862/1‑71 (MQ=255)             cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                                    >  1:315129/1‑71 (MQ=255)             cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                                    >  1:2712293/1‑71 (MQ=255)             cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                                    >  1:3037038/1‑71 (MQ=255)             cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                                    >  1:2047732/1‑71 (MQ=255)                         gagaTCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGa                                  >  1:1390462/1‑61 (MQ=255)                         gagaTCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGa                                  >  1:4265751/1‑61 (MQ=255)                         gagaTCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGa                                  >  1:2459931/1‑61 (MQ=255)                         gagaTCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGa                                  >  1:2075584/1‑61 (MQ=255)                         gagaTCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGa                                  >  1:531104/1‑61 (MQ=255)                         gagaTCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGa                                  >  1:3615645/1‑61 (MQ=255)                         gagaTCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGa                                  >  1:3755029/1‑61 (MQ=255)                         gagaTCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGa                                  >  1:837341/1‑61 (MQ=255)                         gagaTCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGa                                  >  1:1648489/1‑61 (MQ=255)                         gagaTCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGa                        >  1:770799/1‑71 (MQ=255)                         gagaTCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGa                        >  1:1488487/1‑71 (MQ=255)                                   tACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGgag                <  1:2653277/69‑1 (MQ=255)                                   tACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGgag                <  1:900706/69‑1 (MQ=255)                                   tACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGgag                <  1:4035042/69‑1 (MQ=255)                                   tACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGgag                <  1:2969017/69‑1 (MQ=255)                                     cAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGagaag             <  1:3353477/70‑1 (MQ=255)                                     cAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGagaag             >  1:3355294/1‑70 (MQ=255)                                     cAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGagaag             >  1:3364180/1‑70 (MQ=255)                                     cAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGagaag             >  1:3514186/1‑70 (MQ=255)                                     cAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGagaag             >  1:76769/1‑70 (MQ=255)                                     cAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGagaag             >  1:1035921/1‑70 (MQ=255)                                     cAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGagaag             >  1:1581032/1‑70 (MQ=255)                                     cAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGagaag             >  1:1836199/1‑70 (MQ=255)                                     cAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGagaag             <  1:2255289/70‑1 (MQ=255)                                     cAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGagaag             <  1:2471906/70‑1 (MQ=255)                                     cAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGagaag             >  1:2692532/1‑70 (MQ=255)                                     cAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGagaag             >  1:2839440/1‑70 (MQ=255)                                     cAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGagaag             <  1:1113273/70‑1 (MQ=255)                                     cAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGagaag             >  1:2867525/1‑70 (MQ=255)                                     cAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCC‑‑‑‑AAATAATTGCGATGGATTTTGATACGGGAGAAGAATGt        <  1:3260894/71‑1 (MQ=255)                                     cAGGCCGCCGCCAGAGACAACCCCGCTGTCGCCAAAGAaaaaat                                       >  1:1428613/1‑44 (MQ=38)                                     cAGGCCGCCGCCAGAGACAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGagaag             >  1:3262507/1‑70 (MQ=255)                                       ggCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGTAtttt                          <  1:1896111/55‑1 (MQ=255)                                       ggCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGAtttt                          <  1:140705/55‑1 (MQ=255)                                       ggCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGAtttt                          <  1:139180/55‑1 (MQ=255)                                        gccgccgccAGAGCCAACCCGGCTGTCGCCAAAGa                                             <  1:4150347/35‑1 (MQ=38)                                        gccgccgccAGAGCCAACCCCGCTGTCGCCAAAGa                                             <  1:2427440/35‑1 (MQ=255)                                        gccgccgccAGAGCCAACCCCGCTGTCGCCAAAGa                                             <  1:298089/35‑1 (MQ=255)                                        gccgccgccAGAGCCAACCCCGCTGTCGCCAAAGa                                             <  1:818148/35‑1 (MQ=255)                                        gccgccgccAGAGCCAACCCCGCTGTCGCCAAAGa                                             <  1:4096095/35‑1 (MQ=255)                                        gccgccgccAGAGCCAACCCCGCTGTCGCCAAAGa                                             <  1:1537087/35‑1 (MQ=255)                                         ccgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGt        <  1:1926946/71‑1 (MQ=255)                                         ccgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGAAACGGGAGAAGAATGt        <  1:3935708/71‑1 (MQ=255)                                          cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGa                              >  1:2885913/1‑48 (MQ=255)                                          cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGa                              >  1:4066864/1‑48 (MQ=255)                                          cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGa                              >  1:4369587/1‑48 (MQ=255)                                          cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGa                              >  1:1378278/1‑48 (MQ=255)                                          cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGa                              >  1:3674396/1‑48 (MQ=255)                                          cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGa                              >  1:1639004/1‑48 (MQ=255)                                          cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGa                              >  1:3024300/1‑48 (MQ=255)                                          cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGa                              >  1:2974242/1‑48 (MQ=255)                                          cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGa                              >  1:2915982/1‑48 (MQ=255)                                          cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGt        <  1:2566094/70‑1 (MQ=255)                                          cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc       <  1:4006373/71‑1 (MQ=255)                                               ccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTAc  <  1:4026343/71‑1 (MQ=255)                                               ccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTAc  <  1:371686/71‑1 (MQ=255)                                               ccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTAc  <  1:187876/71‑1 (MQ=255)                                               ccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTAc  <  1:2480875/71‑1 (MQ=255)                                               ccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTAc  <  1:839280/71‑1 (MQ=255)                                               ccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTAc  <  1:4309980/71‑1 (MQ=255)                                                       aaCCCCGCTGTCGCCAAAGAAATAATTGCGATGGAGTTTGATACGGGagaag             <  1:4051199/52‑1 (MQ=255)                                                           ccGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGt        >  1:1188853/1‑53 (MQ=255)                                                           ccGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc       >  1:99869/1‑54 (MQ=255)                                                           ccGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc       >  1:758002/1‑54 (MQ=255)                                                           ccGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc       >  1:4213005/1‑54 (MQ=255)                                                           ccGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc       >  1:3746442/1‑54 (MQ=255)                                                           ccGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc       >  1:3183768/1‑54 (MQ=255)                                                           ccGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc       >  1:3095716/1‑54 (MQ=255)                                                           ccGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc       >  1:1654642/1‑54 (MQ=255)                                                           ccGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc       >  1:1077877/1‑54 (MQ=255)                                                              |                                                        ACGGCAGGTGTCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTAC  >  minE/1081797‑1081913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑