Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,097,909	T→C	intergenic (+32/‑397)	ydiK → / → ydiL	predicted inner membrane protein/conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,097,909	0	T	C	100.0%	60.4 / NA	17	intergenic (+32/‑397)	ydiK/ydiL	predicted inner membrane protein/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/10);  total (7/10)

GAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGT  >  minE/1097860‑1097976                                                  |                                                                    gagaAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGCTAACCACCGCTAATTGATAtt                                                >  1:1489049/1‑71 (MQ=255) gagaAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGCTAACCACCGCTAATTGATAtt                                                >  1:601018/1‑71 (MQ=255) gagaAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGCTAACCACCGCTAATTGATAtt                                                >  1:51232/1‑71 (MQ=255) gagaAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGCTAACCACCGCTAATTGATAtt                                                >  1:4289150/1‑71 (MQ=255)     aaCCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGCTAACCACCGCTAATTGATATttgct                                            <  1:2880996/71‑1 (MQ=255)       ccGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGCTAACCACCGCTAATTGATATttgcttg                                          <  1:292154/71‑1 (MQ=255)                         atcaGGCGGCTCTGCCGCCTGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATAttt                           <  1:2136678/68‑1 (MQ=255)                         atcaGGCGGCTCTGCCGCCTGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATAttt                           <  1:225907/68‑1 (MQ=255)                         atcaGGCGGCTCTGCCGCCTGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATAttt                           <  1:3009616/68‑1 (MQ=255)                              gcggcTCTGCCGCCTGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCt                    >  1:220649/1‑70 (MQ=255)                              gcggcTCTGCCGCCTGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCt                    >  1:3700284/1‑70 (MQ=255)                              gcggcTCTGCCGCCTGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCt                    >  1:1733570/1‑70 (MQ=255)                                     tGCCGCCTGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGc              <  1:2289637/69‑1 (MQ=255)                                     tGCCGCCTGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGc              <  1:3515856/69‑1 (MQ=255)                                     tGCCGCCTGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGc              <  1:1250056/69‑1 (MQ=255)                                               ttGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGgt  <  1:2200453/71‑1 (MQ=255)                                               ttGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGgt  <  1:1896985/71‑1 (MQ=255)                                                  |                                                                    GAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGT  >  minE/1097860‑1097976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑