Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,099,149	A→G	T130A (ACA→GCA)	ydiM →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,099,149	0	A	G	98.8%	304.2 / ‑4.1	81	T130A (ACA→GCA)	ydiM	predicted transporter
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (49/31);  total (49/32)
Fisher's exact test for biased strand distribution p-value = 3.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

TGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTACCGCTAATC  >  minE/1099081‑1099208                                                                     |                                                            tGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCa                                                           <  1:307072/71‑1 (MQ=255) tGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCa                                                           <  1:2835329/71‑1 (MQ=255) tGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCa                                                           <  1:4273287/71‑1 (MQ=255) tGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCa                                                           <  1:2087005/71‑1 (MQ=255) tGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCa                                                           <  1:192871/71‑1 (MQ=255)   gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc                                                         >  1:3686556/1‑71 (MQ=255)   gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc                                                         >  1:3449099/1‑71 (MQ=255)   gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc                                                         >  1:3073291/1‑71 (MQ=255)   gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc                                                         >  1:435598/1‑71 (MQ=255)   gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc                                                         >  1:4381364/1‑71 (MQ=255)      aaCAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCaa                                                      <  1:4355323/71‑1 (MQ=255)                      cAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGc                                       <  1:761609/70‑1 (MQ=255)                              tATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTaaa                                         >  1:4138217/1‑60 (MQ=255)                              tATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTaaa                                         >  1:4379531/1‑60 (MQ=255)                              tATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTaaa                                         >  1:2008784/1‑60 (MQ=255)                              tATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAg                                        >  1:1802680/1‑61 (MQ=255)                              tATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAg                                        >  1:2477758/1‑61 (MQ=255)                              tATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAg                                        >  1:1498160/1‑61 (MQ=255)                              tATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAg                                        >  1:1570461/1‑61 (MQ=255)                              tATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAg                                        >  1:3061838/1‑61 (MQ=255)                              tATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAg                                        >  1:1784206/1‑61 (MQ=255)                              tATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAg                                        >  1:1849361/1‑61 (MQ=255)                              tATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAg                                        >  1:1193981/1‑61 (MQ=255)                              tATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTc                              >  1:1331303/1‑71 (MQ=255)                              tATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTc                              >  1:1733403/1‑71 (MQ=255)                              tATCCCAGTTTGATGGAAGCTTTTCCACGCTCACATGGGGCAGCCAATATTTTAATTAAAg                                        >  1:1789370/1‑61 (MQ=255)                              tATCCCAGTTTGATGGAAGATTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTc                              >  1:4309170/1‑71 (MQ=255)                               aTCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTc                              >  1:4227661/1‑70 (MQ=255)                               aTCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTc                              >  1:1295551/1‑70 (MQ=255)                               aTCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTc                              >  1:1829848/1‑70 (MQ=255)                               aTCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTc                              >  1:1288794/1‑70 (MQ=255)                               aTCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCA‑TTGTTTc                              >  1:1445414/1‑69 (MQ=255)                               aTCACAGTTTGATGGAAGCTTTTCCACGCTCACGTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTc                              >  1:1538937/1‑70 (MQ=255)                                  ccAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAg                           >  1:2868107/1‑70 (MQ=255)                                  ccAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAg                           >  1:3111063/1‑70 (MQ=255)                                      tttGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCAtttat                                 <  1:4090118/60‑3 (MQ=255)                                      tttGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGt                                 <  1:3293640/60‑1 (MQ=255)                                      tttGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGt                                 <  1:429129/60‑1 (MQ=255)                                      tttGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGt                                 <  1:4035210/60‑1 (MQ=255)                                      tttGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGt                                 <  1:2609932/60‑1 (MQ=255)                                      tttGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGt                                 <  1:196261/60‑1 (MQ=255)                                      tttGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGt                                 <  1:1730167/60‑1 (MQ=255)                                      tttGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGt                                 <  1:203514/60‑1 (MQ=255)                                      tttGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGt                                 <  1:539560/60‑1 (MQ=255)                                        tGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGt                                 <  1:1921921/58‑1 (MQ=255)                                          aTGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGc                                       <  1:98278/50‑1 (MQ=255)                                             gAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtttt                >  1:4060421/1‑70 (MQ=255)                                             gAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtttt                >  1:375277/1‑70 (MQ=255)                                             gAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtttt                >  1:3056139/1‑70 (MQ=255)                                             gAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtttt                >  1:2007995/1‑70 (MQ=255)                                             gAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtttt                >  1:3742069/1‑70 (MQ=255)                                             gAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtttt                >  1:2787937/1‑70 (MQ=255)                                             gAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtttt                >  1:3389555/1‑70 (MQ=255)                                             gAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtttt                >  1:3612207/1‑70 (MQ=255)                                             gAAGCTTTTCCACGCTCACCTGGAGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtttt                >  1:2055150/1‑70 (MQ=255)                                               aGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTttat             >  1:1827865/1‑71 (MQ=255)                                               aGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTttat             >  1:533652/1‑71 (MQ=255)                                               aGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTttat             >  1:373309/1‑71 (MQ=255)                                               aGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTttat             >  1:3342416/1‑71 (MQ=255)                                               aGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTttat             >  1:3053737/1‑71 (MQ=255)                                               aGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTttat             >  1:2093752/1‑71 (MQ=255)                                               aGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTttat             >  1:2200194/1‑71 (MQ=255)                                                gCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCGGCGGACAATTTttatt            <  1:2896813/71‑1 (MQ=255)                                                gCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTttatt            <  1:696019/71‑1 (MQ=255)                                                gCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTttatt            <  1:2790738/71‑1 (MQ=255)                                                gCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTttatt            <  1:2281979/71‑1 (MQ=255)                                                gCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTttatt            <  1:1961545/71‑1 (MQ=255)                                                gCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTttatt            <  1:185682/71‑1 (MQ=255)                                                gCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTttatt            <  1:1236082/71‑1 (MQ=255)                                                gCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTttatt            <  1:1134095/71‑1 (MQ=255)                                                 cTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTttatta           >  1:2469127/1‑71 (MQ=255)                                                   tttCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTAcc         >  1:2940948/1‑71 (MQ=255)                                                   tttCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTAcc         >  1:2798397/1‑71 (MQ=255)                                                   tttCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTAcc         >  1:2025011/1‑71 (MQ=255)                                                   tttCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTAcc         >  1:824457/1‑71 (MQ=255)                                                         cGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTACCGCTAAt   <  1:4158954/71‑1 (MQ=255)                                                         cGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTACCGCTAAt   <  1:2017509/71‑1 (MQ=255)                                                         cGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTACCGCTAAt   <  1:724980/71‑1 (MQ=255)                                                          gCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTACCGCTAAt   <  1:1985658/70‑1 (MQ=255)                                                          gCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTACCGCTAAt   <  1:1213583/70‑1 (MQ=255)                                                          gCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTACCGCTAATc  <  1:1073357/71‑1 (MQ=255)                                                                     |                                                            TGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTACCGCTAATC  >  minE/1099081‑1099208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑