Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,151,211	A→G	V2V (GTA→GTG)	ydjQ →	endonuclease of nucleotide excision repair

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,151,211	0	A	G	100.0%	249.2 / NA	64	V2V (GTA→GTG)	ydjQ	endonuclease of nucleotide excision repair
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/58);  total (6/58)

TAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTACGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCG  >  minE/1151153‑1151269                                                           |                                                           tAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAAc                                                 <  1:159231/70‑1 (MQ=255) tAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAAc                                                 <  1:439008/70‑1 (MQ=255) tAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAAc                                                 <  1:4224448/70‑1 (MQ=255) tAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAAc                                                 <  1:2153766/70‑1 (MQ=255) tAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAAc                                                 <  1:3869770/70‑1 (MQ=255) tAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAAc                                                 <  1:569655/70‑1 (MQ=255) tAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAAc                                                 <  1:2775490/70‑1 (MQ=255) tAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAAc                                                 <  1:629294/70‑1 (MQ=255) tAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAAc                                                 <  1:1651519/70‑1 (MQ=255) tAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAAc                                                 <  1:789365/70‑1 (MQ=255) tAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAAc                                                 <  1:848269/70‑1 (MQ=255) tAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAAc                                                 <  1:3850702/70‑1 (MQ=255) tAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAAc                                                 <  1:382196/70‑1 (MQ=255) tAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAAc                                                 <  1:130153/70‑1 (MQ=255) tAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAAc                                                 <  1:3745383/70‑1 (MQ=255) tAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAAc                                                 <  1:3424519/70‑1 (MQ=255)  aaTCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAAc                                                 <  1:117434/69‑1 (MQ=255)  aaTCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACt                                                <  1:4005317/70‑1 (MQ=255)  aaTCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACt                                                <  1:2007347/70‑1 (MQ=255)           ccGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCg                                                          <  1:2396069/51‑1 (MQ=255)           ccGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCg                                                          <  1:536711/51‑1 (MQ=255)           ccGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCg                                                          <  1:553133/51‑1 (MQ=255)           ccGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCg                                                          <  1:1321459/51‑1 (MQ=255)           ccGCCTGTTACACTGGATAGACAACCAGCATTCGGAGTCAACAGTGGTGCg                                                          <  1:135013/51‑1 (MQ=255)                    acacTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAAttt                              >  1:2455141/1‑70 (MQ=255)                    acacTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAAttt                              >  1:2222574/1‑70 (MQ=255)                       caggATAGATAACCAGCATTCGGAGTCAACAGTGCTGCGGCGt                                                      >  1:3427707/3‑43 (MQ=255)                       cTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAg                          >  1:2355751/1‑71 (MQ=255)                       cTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAg                          >  1:2249192/1‑71 (MQ=255)                       cTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAg                          >  1:126537/1‑71 (MQ=255)                                    ctgCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAAttt              <  1:906384/68‑1 (MQ=255)                                       cATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAAttt              <  1:4374644/67‑1 (MQ=255)                                       cATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAAttt              <  1:1426241/67‑1 (MQ=255)                                       cATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAAttt              <  1:29690/67‑1 (MQ=255)                                       cATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAAttt              <  1:4320452/67‑1 (MQ=255)                                       cATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAAttt              <  1:1582173/67‑1 (MQ=255)                                       cATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAAttt              <  1:3572328/67‑1 (MQ=255)                                       cATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAAttt              <  1:2533058/67‑1 (MQ=255)                                       cATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAAttt              <  1:1832390/67‑1 (MQ=255)                                       cATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAAttt              <  1:625811/67‑1 (MQ=255)                                       cATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAAttt              <  1:490078/67‑1 (MQ=255)                                       cATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAAttt              <  1:2484577/67‑1 (MQ=255)                                       cATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAAttt              <  1:3905216/67‑1 (MQ=255)                                       cATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAAttt              <  1:2697202/67‑1 (MQ=255)                                        aTTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAAttt              <  1:407107/66‑1 (MQ=255)                                        aTTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATg           <  1:4006163/69‑1 (MQ=255)                                           cGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAata       <  1:2087847/70‑1 (MQ=255)                                               gTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCg  <  1:213081/71‑1 (MQ=255)                                               gTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCg  <  1:4206958/71‑1 (MQ=255)                                               gTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCg  <  1:678183/71‑1 (MQ=255)                                               gTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCg  <  1:803891/71‑1 (MQ=255)                                               gTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCg  <  1:1073165/71‑1 (MQ=255)                                               gTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCg  <  1:4238325/71‑1 (MQ=255)                                               gTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCg  <  1:2675128/71‑1 (MQ=255)                                               gTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCg  <  1:3164009/71‑1 (MQ=255)                                               gTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCg  <  1:2755532/71‑1 (MQ=255)                                               gTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCg  <  1:2800172/71‑1 (MQ=255)                                               gTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCg  <  1:2855181/71‑1 (MQ=255)                                               gTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCg  <  1:3645205/71‑1 (MQ=255)                                               gTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCg  <  1:3516178/71‑1 (MQ=255)                                               gTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCg  <  1:3111019/71‑1 (MQ=255)                                               gTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCg  <  1:3169039/71‑1 (MQ=255)                                                tCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCg  <  1:2920613/70‑1 (MQ=255)                                                tCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCg  <  1:3556237/70‑1 (MQ=255)                                                           |                                                           TAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTACGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCG  >  minE/1151153‑1151269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑