Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	91,462	T→C	S56P (TCT→CCT)	lpxC →	UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	91,462	0	T	C	100.0%	341.6 / NA	89	S56P (TCT→CCT)	lpxC	UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/50);  total (39/50)

ACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCG  >  minE/91399‑91525                                                                |                                                                acCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGc                                                           >  1:277502/1‑70 (MQ=255) acCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCg                                                          >  1:495801/1‑71 (MQ=255) acCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCg                                                          >  1:1052715/1‑71 (MQ=255) acCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCg                                                          >  1:77104/1‑71 (MQ=255) acCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCg                                                          >  1:1133035/1‑71 (MQ=255) acCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCg                                                          >  1:1195898/1‑71 (MQ=255) acCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCg                                                          >  1:4118917/1‑71 (MQ=255) acCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCg                                                          >  1:1790896/1‑71 (MQ=255) acCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCg                                                          >  1:670576/1‑71 (MQ=255)  ccGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGt                                                         <  1:303744/71‑1 (MQ=255)  ccGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGt                                                         <  1:30324/71‑1 (MQ=255)                 gtcgCACCGACTTGAATCCCCCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                           >  1:710969/1‑70 (MQ=255)                 gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                           <  1:617404/70‑1 (MQ=255)                 gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                           <  1:3993764/70‑1 (MQ=255)                 gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                           <  1:3302273/70‑1 (MQ=255)                 gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                           <  1:2927431/70‑1 (MQ=255)                 gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                           <  1:2368332/70‑1 (MQ=255)                 gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                           <  1:3320135/70‑1 (MQ=255)                 gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                           <  1:523616/70‑1 (MQ=255)                 gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                           <  1:517690/70‑1 (MQ=255)                 gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                           <  1:768143/70‑1 (MQ=255)                 gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                           <  1:2007738/70‑1 (MQ=255)                 gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                           <  1:761753/70‑1 (MQ=255)                 gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                           <  1:1945429/70‑1 (MQ=255)                 gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                           <  1:1687252/70‑1 (MQ=255)                 gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                           <  1:3567699/70‑1 (MQ=255)                 gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                           <  1:1526852/70‑1 (MQ=255)                 gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                           <  1:491969/70‑1 (MQ=255)                 gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                           <  1:984593/70‑1 (MQ=255)                 gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                           <  1:1349142/70‑1 (MQ=255)                 gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                           >  1:452971/1‑70 (MQ=255)                 gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                           <  1:3816241/70‑1 (MQ=255)                  tcgCACCGACTTGAATCCACCGGTAGATTTCCCGTCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                           <  1:4180691/69‑1 (MQ=255)                  tcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTa                                         <  1:3932759/71‑1 (MQ=255)                  tcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTa                                         <  1:3516435/71‑1 (MQ=255)                  tcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTa                                         <  1:3567421/71‑1 (MQ=255)                  tcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTa                                         <  1:3317609/71‑1 (MQ=255)                  tcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTa                                         <  1:3618765/71‑1 (MQ=255)                  tcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTa                                         <  1:3060514/71‑1 (MQ=255)                  tcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTa                                         <  1:1092635/71‑1 (MQ=255)                  tcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTa                                         <  1:2256615/71‑1 (MQ=255)                  tcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTa                                         <  1:935393/71‑1 (MQ=255)                      aCCGACTTGAATCCACCGGTAGGTTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACgtg                                     >  1:3982743/1‑71 (MQ=255)                      aCCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTTTGCGTGATACCATGCTCTGTACgtg                                     <  1:934769/71‑1 (MQ=255)                      aCCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACgtg                                     >  1:551856/1‑71 (MQ=255)                      aCCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACgtg                                     >  1:1311113/1‑71 (MQ=255)                      aCCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACgtg                                     >  1:1486116/1‑71 (MQ=255)                      aCCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACgtg                                     <  1:3756064/71‑1 (MQ=255)                      aCCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACgtg                                     <  1:3613979/71‑1 (MQ=255)                      aCCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACgtg                                     <  1:1752225/71‑1 (MQ=255)                      aCCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACgtg                                     >  1:3549933/1‑71 (MQ=255)                      aCCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACgtg                                     <  1:1766835/71‑1 (MQ=255)                      aCCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACgtg                                     <  1:2043684/71‑1 (MQ=255)                      aCCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACgtg                                     <  1:3420316/71‑1 (MQ=255)                      aCCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACgtg                                     >  1:744935/1‑71 (MQ=255)                      aCCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACgtg                                     <  1:3977396/71‑1 (MQ=255)                      aCCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACgtg                                     <  1:2659343/71‑1 (MQ=255)                      aCCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACgtg                                     <  1:2716346/71‑1 (MQ=255)                      aCCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACgtg                                     <  1:2899050/71‑1 (MQ=255)                      aCCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGAGACCATGCTCTGTACgtg                                     <  1:4183838/71‑1 (MQ=255)                       ccGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACgtgt                                    >  1:3402201/1‑71 (MQ=255)                       ccGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACgtgt                                    >  1:31554/1‑71 (MQ=255)                       ccGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACgtgt                                    >  1:4352201/1‑71 (MQ=255)                       ccGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACgtgt                                    >  1:992006/1‑71 (MQ=255)                          aCTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTg                                 <  1:1003757/71‑1 (MQ=255)                          aCTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTg                                 <  1:1295113/71‑1 (MQ=255)                              gAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGt                                                         >  1:3333007/1‑43 (MQ=255)                              gAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGt                                                         >  1:1540405/1‑43 (MQ=255)                              gAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGt                                                         >  1:1581173/1‑43 (MQ=255)                              gAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGt                                                         >  1:1427967/1‑43 (MQ=255)                              gAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGt                                                         >  1:1802/1‑43 (MQ=255)                              gAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGt                                                         >  1:2602418/1‑43 (MQ=255)                              gAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGt                                                         >  1:3406760/1‑43 (MQ=255)                              gAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGt                                                         >  1:2986647/1‑43 (MQ=255)                                    aCCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGc                       <  1:2810869/71‑1 (MQ=255)                                    aCCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGc                       <  1:510254/71‑1 (MQ=255)                                    aCCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGc                       <  1:3500740/71‑1 (MQ=255)                                                   ggCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGAttt        >  1:531306/1‑71 (MQ=255)                                                   ggCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGAttt        >  1:312547/1‑71 (MQ=255)                                                   ggCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGAttt        >  1:208634/1‑71 (MQ=255)                                                   ggCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGAttt        >  1:204445/1‑71 (MQ=255)                                                   ggCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGAttt        >  1:196938/1‑71 (MQ=255)                                                   ggCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGAttt        >  1:3778799/1‑71 (MQ=255)                                                   ggCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGAttt        >  1:3810893/1‑71 (MQ=255)                                                   ggCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGAtt         >  1:1900381/1‑70 (MQ=255)                                                         tGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCg  <  1:1050093/71‑1 (MQ=255)                                                         tGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCg  <  1:4208938/71‑1 (MQ=255)                                                          gCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCg  >  1:851596/1‑70 (MQ=255)                                                          gCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCg  >  1:4118033/1‑70 (MQ=255)                                                                |                                                                ACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCG  >  minE/91399‑91525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑