Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,252,050	T→C	K6K (AAA→AAG)	yebC ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,252,050	0	T	C	100.0%	52.8 / NA	15	K6K (AAA→AAG)	yebC	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/0);  total (15/0)

ATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAAC  >  minE/1252032‑1252117                   |                                                                    atctCTGGTGTTGGCCCACTTACTATGACCTGCGATAAAAATATCTCCAGATAGCCCTGCCTGTTCAgg                   >  1:4158092/4‑69 (MQ=255) aTGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAgg                   >  1:102306/1‑69 (MQ=255) aTGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAgg                   >  1:1137671/1‑69 (MQ=255) aTGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAgg                   >  1:1208951/1‑69 (MQ=255) aTGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAgg                   >  1:1348316/1‑69 (MQ=255) aTGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAgg                   >  1:1974208/1‑69 (MQ=255) aTGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAgg                   >  1:2303370/1‑69 (MQ=255) aTGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAgg                   >  1:3257257/1‑69 (MQ=255) aTGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAgg                   >  1:3713064/1‑69 (MQ=255) aTGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAgg                   >  1:497444/1‑69 (MQ=255) aTGTCTGGTGTTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAgg                   >  1:551564/1‑69 (MQ=255) aTGTCTGGTGTTGGCCCACTTACTATGAACTGCCATAAAAATATATACAGATAGCCCTGCCTGTTCAgg                   >  1:398294/1‑69 (MQ=255)                ccACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCcctgcctga                        >  1:910945/1‑48 (MQ=255)                ccACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAAc  >  1:1006195/1‑71 (MQ=255)                ccACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAAc  >  1:2936818/1‑71 (MQ=255)                   |                                                                    ATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAAC  >  minE/1252032‑1252117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑