Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,253,716	C→T	A241A (GCG→GCA)	aspS ←	aspartyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,253,716	0	C	T	100.0%	171.3 / NA	50	A241A (GCG→GCA)	aspS	aspartyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/9);  total (41/9)

CCACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATT  >  minE/1253652‑1253756                                                                 |                                         ccACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTgcggtgcggt                                     >  1:2478292/1‑70 (MQ=255) ccACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTgcggtgcggt                                     >  1:2714734/1‑70 (MQ=255) ccACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTgcggtgcggt                                     >  1:270278/1‑70 (MQ=255) ccACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTgcggtgcggt                                     >  1:2743822/1‑70 (MQ=255) ccACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTgcggtgcggt                                     >  1:1908105/1‑70 (MQ=255) ccACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTgcggtgcggt                                     >  1:2158895/1‑70 (MQ=255) ccACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTgcggtgcggt                                     >  1:3610661/1‑70 (MQ=255) ccACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTgcggtgcggt                                     >  1:813376/1‑70 (MQ=255) ccACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTgcggtgcggt                                     >  1:2355850/1‑70 (MQ=255) ccACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTgcggtgcggt                                     >  1:1440014/1‑70 (MQ=255) ccACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTgcggtgcggt                                     >  1:4330102/1‑70 (MQ=255) ccACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTgcggtgcggt                                     >  1:305662/1‑70 (MQ=255) ccACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTgcggtgcggt                                     >  1:2470360/1‑70 (MQ=255) ccACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACCCACTTgcggtgcggt                                     >  1:2730998/1‑70 (MQ=255)                ccAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGTAGTTTc                      <  1:563319/70‑1 (MQ=255)                ccAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTc                      <  1:2664626/70‑1 (MQ=255)                ccAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTc                      <  1:3329245/70‑1 (MQ=255)                ccAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTc                      <  1:2006369/70‑1 (MQ=255)                ccAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTc                      <  1:327551/70‑1 (MQ=255)                ccAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTc                      <  1:1860040/70‑1 (MQ=255)                ccAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTc                      <  1:864132/70‑1 (MQ=255)                ccAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTc                      <  1:1603185/70‑1 (MQ=255)                ccAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTc                      <  1:1047421/70‑1 (MQ=255)                     cacaGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATc                >  1:2076772/1‑71 (MQ=255)                     cacaGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATc                >  1:2858822/1‑71 (MQ=255)                     cacaGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATc                >  1:3052410/1‑71 (MQ=255)                     cacaGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATc                >  1:305811/1‑71 (MQ=255)                     cacaGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATc                >  1:3159756/1‑71 (MQ=255)                     cacaGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATc                >  1:1974170/1‑71 (MQ=255)                     cacaGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATc                >  1:1969173/1‑71 (MQ=255)                     cacaGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATc                >  1:1868243/1‑71 (MQ=255)                     cacaGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATc                >  1:1421491/1‑71 (MQ=255)                     cacaGATGACGCACCAGCGCTTACATCACTTCACGCACTtgcggtgcgg                                      >  1:2764749/1‑49 (MQ=255)                                cACCAGCGCTTCCATCTCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGGTTCCACATCGATCTGAGTa      >  1:3560243/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGt       >  1:1922856/1‑69 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:3549931/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:3737075/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:914197/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:4063040/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:4233794/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:4379798/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:441250/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:600308/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:80411/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:893671/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:1003002/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:2241555/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:102580/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:1701564/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:175626/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:1944067/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:1992462/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:2009370/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:2265332/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:3037423/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:306542/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:322417/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:3345244/1‑70 (MQ=255)                                cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTa      >  1:3387243/1‑70 (MQ=255)                                    aGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAAtt  >  1:2366561/1‑70 (MQ=255)                                    aGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAAtt  >  1:3529420/1‑70 (MQ=255)                                    aGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAAtt  >  1:542112/1‑70 (MQ=255)                                    aGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAAtt  >  1:661811/1‑70 (MQ=255)                                    aGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAAtt  >  1:3144038/1‑70 (MQ=255)                                    aGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAAtt  >  1:1370602/1‑70 (MQ=255)                                                                 |                                         CCACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATT  >  minE/1253652‑1253756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑