Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,302,228	C→T	V285M (GTG→ATG)	dacD ←	D‑alanyl‑D‑alanine carboxypeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,302,228	0	C	T	100.0%	250.3 / NA	74	V285M (GTG→ATG)	dacD	D‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/35);  total (39/35)

TTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCA  >  minE/1302166‑1302292                                                               |                                                                 ttCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCgtagta                                                           >  1:2352360/1‑70 (MQ=255) ttCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCgtagta                                                           >  1:768978/1‑70 (MQ=255) ttCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCgtagta                                                           >  1:4092963/1‑70 (MQ=255) ttCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTT‑‑CACGGTGCAAAATTTGCATCGTAGTAAAg                                                        >  1:2336810/1‑71 (MQ=255)          gCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTttgtt                                                 <  1:868062/71‑1 (MQ=255)          gCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTttgt                                                  <  1:3518025/70‑1 (MQ=255)           ccATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTttgttg                                                <  1:1297898/71‑1 (MQ=255)           ccATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTttgttg                                                <  1:1643587/71‑1 (MQ=255)           ccATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTttgttg                                                <  1:3128017/71‑1 (MQ=255)            cATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTttgttg                                                <  1:933698/70‑1 (MQ=255)                ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:3740071/1‑70 (MQ=255)                ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:2284506/1‑70 (MQ=255)                ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:2685417/1‑70 (MQ=255)                ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:3134004/1‑70 (MQ=255)                ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:215349/1‑70 (MQ=255)                ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:3411601/1‑70 (MQ=255)                ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:551968/1‑70 (MQ=255)                ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:598662/1‑70 (MQ=255)                ccAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcccc                                            >  1:849890/1‑70 (MQ=255)                  aGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCaa                                         <  1:3706624/71‑1 (MQ=255)                  aGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCaa                                         <  1:1094811/71‑1 (MQ=255)                  aGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCaa                                         <  1:789776/71‑1 (MQ=255)                  aGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCaa                                         <  1:252410/71‑1 (MQ=255)                  aGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCaa                                         <  1:210227/71‑1 (MQ=255)                  aGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCaa                                         <  1:121346/71‑1 (MQ=255)                                  gACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTg                         <  1:3558862/71‑1 (MQ=255)                                  gACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTg                         <  1:1698922/71‑1 (MQ=255)                                  gACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTg                         <  1:761728/71‑1 (MQ=255)                                  gACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTg                         <  1:3797233/71‑1 (MQ=255)                                  gACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTg                         <  1:3742027/71‑1 (MQ=255)                                  gACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTg                         <  1:3467580/71‑1 (MQ=255)                                  gACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTg                         <  1:2473175/71‑1 (MQ=255)                                   aCCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCaa                                         >  1:3092750/1‑54 (MQ=255)                                   aCCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGc                        >  1:2503966/1‑71 (MQ=255)                                   aCCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGc                        >  1:3693980/1‑71 (MQ=255)                                   aCCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGc                        >  1:36662/1‑71 (MQ=255)                                   aCCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGc                        >  1:765389/1‑71 (MQ=255)                                   aCCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGc                        >  1:1904012/1‑71 (MQ=255)                                   aCCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGc                        >  1:2256639/1‑71 (MQ=255)                                   aCCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGc                        >  1:2223748/1‑71 (MQ=255)                                   aCCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGc                        >  1:174177/1‑71 (MQ=255)                                        tttCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctc                     <  1:1198661/69‑1 (MQ=255)                                                ggTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGa           <  1:3975370/71‑1 (MQ=255)                                                    cAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCt        <  1:3916635/70‑1 (MQ=255)                                                    cAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCt        <  1:749553/70‑1 (MQ=255)                                                    cAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCt        <  1:3797726/70‑1 (MQ=255)                                                    cAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCt        <  1:666961/70‑1 (MQ=255)                                                    cAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCt        <  1:680681/70‑1 (MQ=255)                                                    cAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCt        <  1:1215058/70‑1 (MQ=255)                                                    cAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCt        <  1:1556902/70‑1 (MQ=255)                                                    cAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCt        <  1:2578004/70‑1 (MQ=255)                                                    cAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCt        <  1:3144028/70‑1 (MQ=255)                                                    cAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCt        <  1:3127379/70‑1 (MQ=255)                                                     aaaaTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCttt      <  1:1504957/71‑1 (MQ=255)                                                     aaaaTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCttt      <  1:4295269/71‑1 (MQ=255)                                                      aaaTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCtttt     >  1:2227465/1‑71 (MQ=255)                                                      aaaTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCttt      >  1:2715590/1‑70 (MQ=255)                                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:3760156/71‑1 (MQ=255)                                                        aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   <  1:2912365/71‑1 (MQ=255)                                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGa           >  1:3449116/1‑62 (MQ=255)                                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   >  1:3598438/1‑70 (MQ=255)                                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:1756773/1‑71 (MQ=255)                                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:908575/1‑71 (MQ=255)                                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:1072322/1‑71 (MQ=255)                                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:665098/1‑71 (MQ=255)                                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:3956572/1‑71 (MQ=255)                                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:3903094/1‑71 (MQ=255)                                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:3803525/1‑71 (MQ=255)                                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:189856/1‑71 (MQ=255)                                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:3704531/1‑71 (MQ=255)                                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:2719167/1‑71 (MQ=255)                                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:2543040/1‑71 (MQ=255)                                                         tttGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa  >  1:2513238/1‑71 (MQ=255)                                                         tttGCATCGTAGTAAAGTTTTGTTGCCCACAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc   >  1:2571221/1‑70 (MQ=255)                                                               |                                                                 TTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCA  >  minE/1302166‑1302292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑