Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,325,522	T→G	K71T (AAA→ACA)	wcaL ←	predicted glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,325,522	0	T	G	100.0%	221.7 / NA	58	K71T (AAA→ACA)	wcaL	predicted glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (34/24);  total (34/24)

AGCGCCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCG  >  minE/1325459‑1325585                                                                |                                                                aGCGCCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCAc                                                          <  1:587612/71‑1 (MQ=255) aGCGCCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCAc                                                          <  1:2009418/71‑1 (MQ=255) aGCGCCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCAc                                                          <  1:483157/71‑1 (MQ=255)    gcCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACttt                                                       >  1:725531/1‑71 (MQ=255)    gcCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACttt                                                       >  1:3036822/1‑71 (MQ=255)    gcCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACttt                                                       >  1:294245/1‑71 (MQ=255)    gcCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACttt                                                       >  1:3570750/1‑71 (MQ=255)    gcCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACttt                                                       >  1:2281116/1‑71 (MQ=255)    gcCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACttt                                                       >  1:2253696/1‑71 (MQ=255)    gcCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACttt                                                       >  1:4196071/1‑71 (MQ=255)    gcCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACttt                                                       >  1:205515/1‑71 (MQ=255)    gcCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACttt                                                       >  1:705025/1‑71 (MQ=255)    gcCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACttt                                                       >  1:1409064/1‑71 (MQ=255)    gcCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACttt                                                       >  1:1116287/1‑71 (MQ=255)    gcCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACttt                                                       >  1:526875/1‑71 (MQ=255)               aTTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGt                                             >  1:1285483/1‑70 (MQ=255)                  tttACGATGAATGCCGCGCAAGGTCTGGCTGGCTCTGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCg                                          <  1:2400278/70‑1 (MQ=255)                  tttACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCg                                          <  1:1637144/70‑1 (MQ=255)                  tttACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCg                                          <  1:3368680/70‑1 (MQ=255)                  tttACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCg                                          <  1:2351370/70‑1 (MQ=255)                  tttACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCg                                          <  1:3828515/70‑1 (MQ=255)                  tttACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCg                                          <  1:3889917/70‑1 (MQ=255)                  tttACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCg                                          <  1:2159014/70‑1 (MQ=255)                  tttACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCg                                          <  1:1425265/70‑1 (MQ=255)                           aaTGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGTCCGTAGGTTCGTCCTGTAAc                                 >  1:2396374/1‑70 (MQ=255)                           aaTGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAAc                                 >  1:3342432/1‑70 (MQ=255)                           aaTGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAAc                                 >  1:426348/1‑70 (MQ=255)                           aaTGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAAc                                 >  1:3084046/1‑70 (MQ=255)                           aaTGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAAc                                 >  1:1083328/1‑70 (MQ=255)                           aaTGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAAc                                 >  1:2277771/1‑70 (MQ=255)                           aaTGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAAc                                 >  1:1730593/1‑70 (MQ=255)                           aaTGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAAc                                 >  1:1789750/1‑70 (MQ=255)                           aaTGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAAc                                 >  1:1217151/1‑70 (MQ=255)                           aaTGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAAc                                 >  1:2183241/1‑70 (MQ=255)                           aaTGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAAc                                 >  1:2256730/1‑70 (MQ=255)                           aaTGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAAc                                 >  1:2665255/1‑70 (MQ=255)                              gCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGc                                                             >  1:626526/1‑39 (MQ=255)                              gCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCa                               >  1:4288695/1‑69 (MQ=255)                              gCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGc                             >  1:810178/1‑71 (MQ=255)                              gCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGc                             >  1:1026055/1‑71 (MQ=255)                              gCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGc                             >  1:1112902/1‑71 (MQ=255)                              gCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGc                             >  1:3348603/1‑71 (MQ=255)                              gCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGc                             >  1:332992/1‑71 (MQ=255)                              gCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGc                             >  1:2975089/1‑71 (MQ=255)                                               tggctCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAgg            >  1:3674440/1‑71 (MQ=255)                                                         gcgcAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCg  <  1:940206/71‑1 (MQ=255)                                                         gcgcAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCg  <  1:972790/71‑1 (MQ=255)                                                         gcgcAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCg  <  1:961559/71‑1 (MQ=255)                                                         gcgcAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCg  <  1:122021/71‑1 (MQ=255)                                                         gcgcAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCg  <  1:4268957/71‑1 (MQ=255)                                                         gcgcAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCg  <  1:4205572/71‑1 (MQ=255)                                                         gcgcAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCg  <  1:3843114/71‑1 (MQ=255)                                                         gcgcAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCg  <  1:3839865/71‑1 (MQ=255)                                                         gcgcAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCg  <  1:3735446/71‑1 (MQ=255)                                                         gcgcAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCg  <  1:3647827/71‑1 (MQ=255)                                                         gcgcAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCg  <  1:2375379/71‑1 (MQ=255)                                                         gcgcAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCg  <  1:2929163/71‑1 (MQ=255)                                                         gcgcAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCg  <  1:3254683/71‑1 (MQ=255)                                                                |                                                                AGCGCCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCG  >  minE/1325459‑1325585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑