Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,439,412	G→A	A297V (GCT→GTT)	ompC ←	outer membrane porin protein C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,439,412	0	G	A	100.0%	258.8 / NA	76	A297V (GCT→GTT)	ompC	outer membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/58);  total (18/58)

TATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTG  >  minE/1439349‑1439477                                                                |                                                                  tatTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGgga                                                            <  1:2787400/71‑1 (MQ=255)         gATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAgg                                                              <  1:903673/61‑1 (MQ=255)         gATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAgg                                                              <  1:3234028/61‑1 (MQ=255)         gATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAgg                                                              <  1:833834/61‑1 (MQ=255)         gATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAgg                                                              <  1:709239/61‑1 (MQ=255)         gATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAgg                                                              <  1:633485/61‑1 (MQ=255)         gATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAgg                                                              <  1:2004663/61‑1 (MQ=255)         gATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAgg                                                              <  1:4225504/61‑1 (MQ=255)         gATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAgg                                                              <  1:2070987/61‑1 (MQ=255)         catatCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAgg                                                              <  1:2219712/60‑1 (MQ=255)          atatCTTCGTCGTCGTAGCCACGACCCAGGTTTTTTCCTTTAGACTGCAGGTAAACCAgg                                                              <  1:1895414/60‑1 (MQ=255)           tatCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGCCGCAGa                                                  >  1:1534614/1‑71 (MQ=255)           tatCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGa                                                  >  1:3255403/1‑71 (MQ=255)           tatCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGa                                                  >  1:3017041/1‑71 (MQ=255)           tatCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGa                                                  >  1:2536404/1‑71 (MQ=255)           tatCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGa                                                  >  1:556353/1‑71 (MQ=255)                          aGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAgg                                                              <  1:4235072/44‑1 (MQ=255)                          aGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGgga                                                            >  1:4297763/1‑46 (MQ=255)                          aGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGAc                                                 >  1:564941/1‑57 (MQ=255)                          aGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTgg                                   >  1:427990/1‑71 (MQ=255)                          aGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTgg                                   >  1:2005096/1‑71 (MQ=255)                          aGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTgg                                   >  1:3993735/1‑71 (MQ=255)                          aGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTgg                                   >  1:3763790/1‑71 (MQ=255)                          aGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTgg                                   >  1:3790745/1‑71 (MQ=255)                          aGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTgg                                   >  1:4318146/1‑71 (MQ=255)                          aGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTgg                                   >  1:3830005/1‑71 (MQ=255)                          aGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTgg                                   >  1:701072/1‑71 (MQ=255)                                            tACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACT‑‑GCAACAGCTTCGa                <  1:3866291/70‑1 (MQ=37)                                              ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:3922429/70‑1 (MQ=255)                                              ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:3936302/70‑1 (MQ=255)                                              ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:789372/70‑1 (MQ=255)                                              ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:357537/70‑1 (MQ=255)                                              ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:3331128/70‑1 (MQ=255)                                              ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:874491/70‑1 (MQ=255)                                              ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:1204353/70‑1 (MQ=255)                                              ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:100303/70‑1 (MQ=255)                                              ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:1414176/70‑1 (MQ=255)                                              ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:14809/70‑1 (MQ=255)                                              ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:2508057/70‑1 (MQ=255)                                              ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:2202664/70‑1 (MQ=255)                                              ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:305579/70‑1 (MQ=255)                                              ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:1491921/70‑1 (MQ=255)                                              ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:2864659/70‑1 (MQ=255)                                              ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:1504343/70‑1 (MQ=255)                                              ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:1576470/70‑1 (MQ=255)                                                         cAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCt     <  1:2466762/70‑1 (MQ=255)                                                         cAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCt     <  1:4039939/70‑1 (MQ=255)                                                         cAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCt     <  1:4336383/70‑1 (MQ=255)                                                         cAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCt     <  1:2136215/70‑1 (MQ=255)                                                         cAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCt     <  1:256417/70‑1 (MQ=255)                                                         cAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCt     <  1:3951020/70‑1 (MQ=255)                                                         cAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCt     <  1:2066607/70‑1 (MQ=255)                                                         cAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCt     <  1:2708074/70‑1 (MQ=255)                                                         cAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCt     <  1:3922170/70‑1 (MQ=255)                                                         cAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCt     <  1:750038/70‑1 (MQ=255)                                                         cAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCt     <  1:3516760/70‑1 (MQ=255)                                                         cAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCt     <  1:76726/70‑1 (MQ=255)                                                           ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCtttt  <  1:2549525/71‑2 (MQ=255)                                                           ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCt     <  1:4172304/68‑1 (MQ=255)                                                           ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:2727511/71‑1 (MQ=255)                                                           ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:3317450/71‑1 (MQ=255)                                                           ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:1364764/71‑1 (MQ=255)                                                           ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:3220874/71‑1 (MQ=255)                                                           ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:3383297/71‑1 (MQ=255)                                                           ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:3441958/71‑1 (MQ=255)                                                           ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:2927203/71‑1 (MQ=255)                                                           ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:2776271/71‑1 (MQ=255)                                                           ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:543918/71‑1 (MQ=255)                                                           ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:474055/71‑1 (MQ=255)                                                           ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:4031151/71‑1 (MQ=255)                                                           ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:3261167/71‑1 (MQ=255)                                                            gTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTc           >  1:4289472/1‑61 (MQ=255)                                                            gTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTc           >  1:4025049/1‑61 (MQ=255)                                                            gTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:3560806/70‑1 (MQ=255)                                                            gTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACCGAGCAACAGCTTCGAAGTTc           >  1:265121/1‑61 (MQ=255)                                                             tAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:2313352/55‑1 (MQ=255)                                                                |                                                                  TATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTG  >  minE/1439349‑1439477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑