Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,509,579	G→A	A42T (GCA→ACA)	ackA →	acetate kinase A and propionate kinase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,509,579	0	G	A	100.0%	283.4 / NA	88	A42T (GCA→ACA)	ackA	acetate kinase A and propionate kinase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (66/22);  total (66/22)

TCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGGCGCCGCT  >  minE/1509514‑1509641                                                                  |                                                               tcatcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGt                                                           <  1:1938297/71‑1 (MQ=255)  catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGt                                                           >  1:2428949/1‑70 (MQ=255)  catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGt                                                           >  1:1858000/1‑70 (MQ=255)  catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:2413946/1‑71 (MQ=255)  catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:3555796/1‑71 (MQ=255)  catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:3463507/1‑71 (MQ=255)  catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:3820556/1‑71 (MQ=255)  catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:3159609/1‑71 (MQ=255)  catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:3044583/1‑71 (MQ=255)  catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:3963229/1‑71 (MQ=255)  catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:3991060/1‑71 (MQ=255)  catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:4335978/1‑71 (MQ=255)  catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:1979094/1‑71 (MQ=255)  catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:1750482/1‑71 (MQ=255)  catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:1584427/1‑71 (MQ=255)  catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:1365884/1‑71 (MQ=255)  catcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCC‑AATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:4059714/1‑71 (MQ=255)   atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:3179997/1‑70 (MQ=255)   atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                          >  1:150389/1‑70 (MQ=255)   atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:1617049/1‑71 (MQ=255)   atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:3122529/1‑71 (MQ=255)   atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:2747999/1‑71 (MQ=255)   atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:806396/1‑71 (MQ=255)   atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:2566066/1‑71 (MQ=255)   atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:3110936/1‑71 (MQ=255)   atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:3951673/1‑71 (MQ=255)   atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:3949877/1‑71 (MQ=255)   atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:145287/1‑71 (MQ=255)   atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:341550/1‑71 (MQ=255)   atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:451537/1‑71 (MQ=255)   atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                         >  1:3482910/1‑71 (MQ=255)     cGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATc                                                        >  1:1937919/1‑70 (MQ=255)     cGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATc                                                        >  1:1932916/1‑70 (MQ=255)     cGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATc                                                        >  1:507841/1‑70 (MQ=255)     cGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATc                                                        >  1:2737716/1‑70 (MQ=255)            gTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatgga                                                 >  1:879246/1‑70 (MQ=255)            gTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatgga                                                 >  1:4042920/1‑70 (MQ=255)            gTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatgga                                                 >  1:2103034/1‑70 (MQ=255)               aaTGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaaaa                                              <  1:1253500/70‑1 (MQ=255)               aaTGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaaaa                                              <  1:319267/70‑1 (MQ=255)               aaTGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaaaa                                              <  1:896078/70‑1 (MQ=255)               aaTGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaaaa                                              <  1:1078260/70‑1 (MQ=255)               aaTGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaaaa                                              <  1:1643180/70‑1 (MQ=255)               aaTGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaaaa                                              <  1:2798729/70‑1 (MQ=255)               aaTGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaaaa                                              <  1:1862877/70‑1 (MQ=255)               aaTGGTGAAAAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaaaa                                              <  1:2571322/70‑1 (MQ=255)                aTGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaaaatg                                            <  1:798496/71‑1 (MQ=255)                           tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:3871286/1‑70 (MQ=255)                           tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:3949944/1‑70 (MQ=255)                           tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:3837900/1‑70 (MQ=255)                           tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:613063/1‑70 (MQ=255)                           tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:3099198/1‑70 (MQ=255)                           tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:445328/1‑70 (MQ=255)                           tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:943369/1‑70 (MQ=255)                           tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:453568/1‑70 (MQ=255)                           tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:958803/1‑70 (MQ=255)                           tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:3401442/1‑70 (MQ=255)                           tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:309731/1‑70 (MQ=255)                           tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:135866/1‑70 (MQ=255)                           tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:2841636/1‑70 (MQ=255)                           tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:136502/1‑70 (MQ=255)                           tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:1752688/1‑70 (MQ=255)                           tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:2736861/1‑70 (MQ=255)                           tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:1774259/1‑70 (MQ=255)                           tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                  >  1:259551/1‑70 (MQ=255)                                             gAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               <  1:3803675/71‑1 (MQ=255)                                             gAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               <  1:1135884/71‑1 (MQ=255)                                             gAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               <  1:1311872/71‑1 (MQ=255)                                             gAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               <  1:766101/71‑1 (MQ=255)                                             gAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               <  1:705077/71‑1 (MQ=255)                                             gAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               <  1:548416/71‑1 (MQ=255)                                             gAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               <  1:2337208/71‑1 (MQ=255)                                             gAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               <  1:4288864/71‑1 (MQ=255)                                             gAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               <  1:3973113/71‑1 (MQ=255)                                             gAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               <  1:3916127/71‑1 (MQ=255)                                             gAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               <  1:3175227/71‑1 (MQ=255)                                               aTGTTTCCACCTGCCCGAAACACGTATCaaatggaaaa                                              <  1:3987126/38‑1 (MQ=255)                                                    tCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg               >  1:2687942/1‑64 (MQ=255)                                                    tCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg         >  1:2386526/1‑70 (MQ=255)                                                    tCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAg          >  1:3738446/1‑69 (MQ=255)                                                    tCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGgc        >  1:434446/1‑71 (MQ=255)                                                    tCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGgc        >  1:129323/1‑71 (MQ=255)                                                    tCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGgc        >  1:1226449/1‑71 (MQ=255)                                                          tGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGGCGCCGCt  >  1:1507107/1‑71 (MQ=255)                                                          tGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGGCGCCGCt  >  1:2765591/1‑71 (MQ=255)                                                          tGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGGCGCCGCt  >  1:2877399/1‑71 (MQ=255)                                                          tGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGGCGCCGCt  >  1:2882669/1‑71 (MQ=255)                                                          tGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGGCGCCGCt  >  1:1172389/1‑71 (MQ=255)                                                                  |                                                               TCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGGCGCCGCT  >  minE/1509514‑1509641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑