Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,511,849	T→C	W373R (TGG→CGG)	pta →	phosphate acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,511,849	0	T	C	100.0%	375.4 / NA	98	W373R (TGG→CGG)	pta	phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/50);  total (48/50)

GGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCG  >  minE/1511785‑1511910                                                                 |                                                              ggTTGACGATCACGAACGTATCGATAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCg                                                         <  1:1970165/71‑1 (MQ=255) ggTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACTCTGACCGGATCg                                                         <  1:3674438/71‑1 (MQ=255) ggTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCg                                                         <  1:2470879/71‑1 (MQ=255) ggTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCg                                                         <  1:2571037/71‑1 (MQ=255) ggTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCg                                                         <  1:2951636/71‑1 (MQ=255) ggTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCg                                                         <  1:135133/71‑1 (MQ=255) ggTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCg                                                         <  1:1889446/71‑1 (MQ=255) ggTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCg                                                         <  1:3968878/71‑1 (MQ=255) ggTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCg                                                         <  1:1577225/71‑1 (MQ=255)  gTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCg                                                         <  1:826807/70‑1 (MQ=255)  gTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGa                                                        <  1:3524038/71‑1 (MQ=255)  gTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGa                                                        <  1:264024/71‑1 (MQ=255)  gTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGa                                                        <  1:3376244/71‑1 (MQ=255)  gTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGa                                                        <  1:1720517/71‑1 (MQ=255)  gTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGa                                                        <  1:3588558/71‑1 (MQ=255)  gTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGa                                                        <  1:1416015/71‑1 (MQ=255)  gTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGa                                                        <  1:143200/71‑1 (MQ=255)  gTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGa                                                        <  1:4001757/71‑1 (MQ=255)    tGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAAt                                                      >  1:3220762/1‑71 (MQ=255)    tGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAAt                                                      >  1:3226090/1‑71 (MQ=255)      aCGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAAtc                                                     <  1:3919339/70‑1 (MQ=255)      aCGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAAtc                                                     <  1:843846/70‑1 (MQ=255)      aCGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAAtc                                                     <  1:3668812/70‑1 (MQ=255)      aCGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAAtc                                                     <  1:3670118/70‑1 (MQ=255)            aCGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACt                                              <  1:2591183/71‑1 (MQ=255)            aCGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACt                                              <  1:182966/71‑1 (MQ=255)            aCGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACt                                              <  1:2110183/71‑1 (MQ=255)             cGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACt                                              <  1:3519426/70‑1 (MQ=255)                 cGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCAc                                         >  1:2307504/1‑71 (MQ=255)                 cGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCAc                                         >  1:4251263/1‑71 (MQ=255)                 cGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCAc                                         >  1:1529770/1‑71 (MQ=255)                       gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGCCCGGATCGAATCTCTGACTGCCACTTCTg                                    >  1:778977/1‑70 (MQ=255)                       gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGAc                                               >  1:3186296/1‑59 (MQ=255)                       gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCt                                     >  1:96187/1‑69 (MQ=255)                       gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCt                                     >  1:110417/1‑69 (MQ=255)                       gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCt                                     >  1:2317738/1‑69 (MQ=255)                       gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCt                                     >  1:3847941/1‑69 (MQ=255)                       gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCt                                     >  1:3526525/1‑69 (MQ=255)                               tCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACt                                              <  1:2001731/52‑1 (MQ=255)                                cAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGa                                                        <  1:1673375/41‑1 (MQ=255)                                   gAATACGTTGCTAACTACATCAACGCTGACCGGATCGAAtc                                                     <  1:882195/41‑1 (MQ=255)                                          ttGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctgtct                >  1:3334737/1‑71 (MQ=255)                                          ttGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctgtct                >  1:3437172/1‑71 (MQ=255)                                          ttGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctgtct                >  1:1763540/1‑71 (MQ=255)                                            gCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTcc              >  1:3314674/1‑71 (MQ=255)                                            gCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTcc              >  1:343672/1‑71 (MQ=255)                                            gCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTcc              >  1:2637701/1‑71 (MQ=255)                                            gCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTcc              >  1:1116066/1‑71 (MQ=255)                                            gCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTcc              >  1:1578082/1‑71 (MQ=255)                                            gCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTcc              >  1:1791262/1‑71 (MQ=255)                                                 cTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCcgtc                        <  1:112433/56‑1 (MQ=255)                                                 cTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCcgtc                        <  1:1658105/56‑1 (MQ=255)                                                  tACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCcgtc                        <  1:1940962/55‑1 (MQ=255)                                                   aCATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGa                                   >  1:2457532/1‑43 (MQ=255)                                                   aCATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGc        >  1:498367/1‑70 (MQ=255)                                                   aCATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGc        >  1:2860454/1‑70 (MQ=255)                                                   aCATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCg       >  1:2439157/1‑71 (MQ=255)                                                   aCATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCg       >  1:4368480/1‑71 (MQ=255)                                                   aCATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCg       >  1:1635633/1‑71 (MQ=255)                                                   aCATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCg       >  1:3459755/1‑71 (MQ=255)                                                   aCATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCg       >  1:23136/1‑71 (MQ=255)                                                   aCATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCg       >  1:2936815/1‑71 (MQ=255)                                                   aCATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCg       >  1:2160517/1‑71 (MQ=255)                                                   aCATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCg       >  1:3182276/1‑71 (MQ=255)                                                   aCATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCAGTCACCGCCTGCg       >  1:183526/1‑71 (MQ=255)                                                    cATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGt      <  1:987437/71‑1 (MQ=255)                                                    cATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGt      <  1:1897469/71‑1 (MQ=255)                                                    cATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGt      <  1:2740709/71‑1 (MQ=255)                                                    cATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGt      <  1:1424426/71‑1 (MQ=255)                                                    cATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGt      <  1:1584006/71‑1 (MQ=255)                                                    cATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGt      <  1:2237111/71‑1 (MQ=255)                                                        aaCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctg                   <  1:1360497/54‑1 (MQ=255)                                                        aaCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctg                   <  1:181874/54‑1 (MQ=255)                                                        aaCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctg                   <  1:234296/54‑1 (MQ=255)                                                        aaCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctg                   <  1:727507/54‑1 (MQ=255)                                                        aaCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctg                   <  1:609759/54‑1 (MQ=255)                                                        aaCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctg                   <  1:3425427/54‑1 (MQ=255)                                                        aaCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctg                   <  1:1657099/54‑1 (MQ=255)                                                        aaCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctg                   <  1:3593819/54‑1 (MQ=255)                                                        aaCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctg                   <  1:3839072/54‑1 (MQ=255)                                                        aaCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctg                   <  1:1592942/54‑1 (MQ=255)                                                        aaCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgact                    >  1:1782993/1‑53 (MQ=255)                                                        aaCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCg  >  1:1740995/1‑71 (MQ=255)                                                        aaCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCg  >  1:937825/1‑71 (MQ=255)                                                        aaCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCg  >  1:1140291/1‑71 (MQ=255)                                                        aaCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCg  >  1:4014757/1‑71 (MQ=255)                                                        aaCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCg  >  1:3684782/1‑71 (MQ=255)                                                        aaCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCg  >  1:3412227/1‑71 (MQ=255)                                                        aaCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCg  >  1:2076885/1‑71 (MQ=255)                                                        aaCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCg  >  1:1265887/1‑71 (MQ=255)                                                        aaCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCg  >  1:1231467/1‑71 (MQ=255)                                                        aaCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCg  >  1:1146366/1‑71 (MQ=255)                                                          cGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctgtc                 >  1:61239/1‑54 (MQ=255)                                                          cGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctgtc                 >  1:3928685/1‑54 (MQ=255)                                                          cGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctgtc                 >  1:1563893/1‑54 (MQ=255)                                                          cGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctgt                  >  1:1510071/1‑53 (MQ=255)                                                           gCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctg                   <  1:1794252/51‑1 (MQ=255)                                                              gACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctg                   <  1:832096/48‑1 (MQ=255)                                                                 |                                                              GGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCG  >  minE/1511785‑1511910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑