BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	144,272	C→A	G194V (GGC→GTC)	yadB ←	glutamyl‑Q tRNA(Asp) synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	144,272	0	C	A	100.0%	188.5 / NA	58	G194V (GGC→GTC)	yadB	glutamyl‑Q tRNA(Asp) synthetase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (37/21); total (37/21)

AGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAATGATCATCAACCACAACAGCCAGGTTG  >  minE/144212‑144307

aGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATg                           <  1:2335796/71‑1 (MQ=255)

aGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATg                           <  1:2582558/71‑1 (MQ=255)

aGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATg                           <  1:4229402/71‑1 (MQ=255)

aGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATg                           <  1:3754971/71‑1 (MQ=255)

aGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATg                           <  1:3206282/71‑1 (MQ=255)

      aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCAGTATTTCTGTAACGACCTGGAAATGatcat                      >  1:1914885/1‑70 (MQ=255)

      aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatcat                      >  1:3266022/1‑70 (MQ=255)

      aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatcat                      >  1:2487428/1‑70 (MQ=255)

      aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatcat                      >  1:2661196/1‑70 (MQ=255)

      aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatcat                      >  1:2724482/1‑70 (MQ=255)

      aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatcat                      >  1:2944163/1‑70 (MQ=255)

      aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatcat                      >  1:3088285/1‑70 (MQ=255)

      aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatcat                      >  1:3161364/1‑70 (MQ=255)

      aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatcat                      >  1:3441952/1‑70 (MQ=255)

      aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatcat                      >  1:4284702/1‑70 (MQ=255)

      aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatcat                      >  1:4307609/1‑70 (MQ=255)

      aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatcat                      >  1:648737/1‑70 (MQ=255)

      aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatcat                      >  1:730764/1‑70 (MQ=255)

      aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatcat                      >  1:737445/1‑70 (MQ=255)

      aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatcat                      >  1:1247514/1‑70 (MQ=255)

      aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatcat                      >  1:143952/1‑70 (MQ=255)

      aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatcat                      >  1:1571472/1‑70 (MQ=255)

      aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatcat                      >  1:1355672/1‑70 (MQ=255)

      aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatcat                      >  1:1651644/1‑70 (MQ=255)

      aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatcat                      >  1:1325332/1‑70 (MQ=255)

      aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatcat                      >  1:1750940/1‑70 (MQ=255)

      aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatcat                      >  1:1859926/1‑70 (MQ=255)

         tGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATTAAc                  >  1:4194074/1‑71 (MQ=255)

         tGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAAc                  >  1:4316900/1‑71 (MQ=255)

         tGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAAc                  >  1:4311950/1‑71 (MQ=255)

         tGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAAc                  >  1:537448/1‑71 (MQ=255)

         tGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAAc                  >  1:62709/1‑71 (MQ=255)

         tGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAAc                  >  1:4189852/1‑71 (MQ=255)

         tGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAAc                  >  1:4067620/1‑71 (MQ=255)

         tGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAAc                  >  1:4038154/1‑71 (MQ=255)

         tGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAAc                  >  1:1030584/1‑71 (MQ=255)

         tGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAAc                  >  1:3669155/1‑71 (MQ=255)

         tGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAAc                  >  1:3501329/1‑71 (MQ=255)

         tGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAAc                  >  1:2452532/1‑71 (MQ=255)

         tGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAAc                  >  1:1906339/1‑71 (MQ=255)

         tGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAAc                  >  1:1664524/1‑71 (MQ=255)

         tGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATT‑‑TGTAACGACCTGGAAATGATCATCAACca                >  1:3563969/1‑71 (MQ=255)

          gCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAAc                  <  1:1279690/70‑1 (MQ=255)

          gCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAAc                  <  1:1153870/70‑1 (MQ=255)

          gCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAAc                  <  1:700318/70‑1 (MQ=255)

          gCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAAc                  <  1:192035/70‑1 (MQ=255)

          gCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAAc                  <  1:3040277/70‑1 (MQ=255)

          gCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAAc                  <  1:411050/70‑1 (MQ=255)

          gCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAAc                  <  1:3768382/70‑1 (MQ=255)

                          aaTCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTg  <  1:1012588/70‑1 (MQ=255)

                          aaTCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTg  <  1:2963702/70‑1 (MQ=255)

                          aaTCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTg  <  1:3265311/70‑1 (MQ=255)

                          aaTCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTg  <  1:1553704/70‑1 (MQ=255)

                          aaTCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTg  <  1:582219/70‑1 (MQ=255)

                          aaTCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTg  <  1:59115/70‑1 (MQ=255)

                          aaTCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTg  <  1:3317685/70‑1 (MQ=255)

                          aaTCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTg  <  1:194925/70‑1 (MQ=255)

                          aaTCAGATCAGCCACACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTg  <  1:1458616/70‑1 (MQ=255)

AGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAATGATCATCAACCACAACAGCCAGGTTG  >  minE/144212‑144307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`