Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,536,834	C→T	M1I (ATG→ATA) †	yfcR ←	predicted fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,536,834	0	C	T	100.0%	272.2 / NA	78	M1I (ATG→ATA) †	yfcR	predicted fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (57/21);  total (57/21)

AGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGG  >  minE/1536773‑1536890                                                              |                                                         aGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCtt                                                 <  1:4262826/71‑1 (MQ=255) aGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCtt                                                 <  1:2467882/71‑1 (MQ=255) aGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCtt                                                 <  1:2021872/71‑1 (MQ=255) aGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCtt                                                 <  1:1781376/71‑1 (MQ=255) aGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCtt                                                 <  1:1421581/71‑1 (MQ=255)      cTGCATACCAGCAGCCCTGCTCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCgg                                            <  1:437341/71‑1 (MQ=255)      cTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCgg                                            <  1:3774428/71‑1 (MQ=255)      cTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCgg                                            <  1:588126/71‑1 (MQ=255)      cTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCgg                                            <  1:1463530/71‑1 (MQ=255)      cTGCATACCAGCAGCCCTGCGCACACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCgg                                            <  1:2005668/71‑1 (MQ=255)           tACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       >  1:3690818/1‑71 (MQ=255)           tACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       >  1:3296299/1‑71 (MQ=255)           tACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       >  1:2389325/1‑71 (MQ=255)           tACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       >  1:1907991/1‑71 (MQ=255)                     ccTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATc                              >  1:1349421/1‑70 (MQ=255)                               cTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       <  1:1629466/51‑1 (MQ=255)                               cTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       <  1:544981/51‑1 (MQ=255)                               cTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       <  1:3906231/51‑1 (MQ=255)                               cTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       <  1:1146080/51‑1 (MQ=255)                               cTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       <  1:4290291/51‑1 (MQ=255)                               cTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       <  1:2042834/51‑1 (MQ=255)                               cTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       <  1:193365/51‑1 (MQ=255)                               cTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       <  1:3499219/51‑1 (MQ=255)                               cTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                       <  1:1428853/51‑1 (MQ=255)                                 aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:3165259/1‑71 (MQ=255)                                 aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:3197437/1‑71 (MQ=255)                                 aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:3647744/1‑71 (MQ=255)                                 aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:3365645/1‑71 (MQ=255)                                 aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:568456/1‑71 (MQ=255)                                 aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:556635/1‑71 (MQ=255)                                 aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:3471795/1‑71 (MQ=255)                                 aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:2587051/1‑71 (MQ=255)                                 aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:3874856/1‑71 (MQ=255)                                 aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:2186266/1‑71 (MQ=255)                                 aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:4038052/1‑71 (MQ=255)                                 aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:1963784/1‑71 (MQ=255)                                 aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:195026/1‑71 (MQ=255)                                 aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:1927049/1‑71 (MQ=255)                                 aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:1701896/1‑71 (MQ=255)                                 aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:1669606/1‑71 (MQ=255)                                 aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:1077697/1‑71 (MQ=255)                                 aCAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGACTCTGCTCTTCATCCACCTGGCAGCTg                 >  1:405805/1‑71 (MQ=255)                                    aaCTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCa                            <  1:3677892/57‑1 (MQ=255)                                    aaCTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCa                            <  1:4077832/57‑1 (MQ=255)                                      cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCAt                               >  1:3603770/1‑52 (MQ=255)                                      cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGc                      >  1:890611/1‑61 (MQ=255)                                      cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGcag                 >  1:399355/1‑64 (MQ=255)                                      cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:571499/1‑70 (MQ=255)                                      cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:4238809/1‑70 (MQ=255)                                      cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:1388477/1‑70 (MQ=255)                                      cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:1684090/1‑70 (MQ=255)                                      cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:2080208/1‑70 (MQ=255)                                      cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:758939/1‑70 (MQ=255)                                      cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:2306531/1‑70 (MQ=255)                                      cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:478202/1‑70 (MQ=255)                                      cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:2950050/1‑70 (MQ=255)                                      cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:3048456/1‑70 (MQ=255)                                      cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:575648/1‑70 (MQ=255)                                      cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc             >  1:3491078/1‑70 (MQ=255)                                      cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCGCTTCATCCACCTGGCAGCTGTTGc             >  1:2907343/1‑70 (MQ=255)                                        tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCa                            >  1:594585/1‑53 (MQ=255)                                        tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                           >  1:745199/1‑54 (MQ=255)                                        tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                           >  1:772441/1‑54 (MQ=255)                                        tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                           >  1:3221485/1‑54 (MQ=255)                                        tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                           >  1:4206002/1‑54 (MQ=255)                                        tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                           >  1:3410880/1‑54 (MQ=255)                                        tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                           >  1:3340864/1‑54 (MQ=255)                                        tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                           >  1:3293772/1‑54 (MQ=255)                                        tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                           >  1:3066389/1‑54 (MQ=255)                                        tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                           >  1:2107712/1‑54 (MQ=255)                                        tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                           >  1:1181021/1‑54 (MQ=255)                                        tttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGCCTCTGCTCTTCATCCAc                           >  1:1063981/1‑54 (MQ=255)                                                  aaCTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCAt                               >  1:3055291/1‑40 (MQ=255)                                                  aaCTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCAtt         >  1:3985678/1‑62 (MQ=255)                                                  aaCTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTgg  >  1:3805312/1‑69 (MQ=255)                                                  aaCTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTgg  >  1:1056798/1‑69 (MQ=255)                                                  aaCTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTgg  >  1:2639003/1‑69 (MQ=255)                                                  aaCTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTgg  >  1:2580314/1‑69 (MQ=255)                                                              |                                                         AGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGG  >  minE/1536773‑1536890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑