BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,680,754	T→C	Y1407C (TAC→TGC)	yfhM ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,680,754	0	T	C	100.0%	252.5 / NA	66	Y1407C (TAC→TGC)	yfhM	conserved hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (36/30); total (36/30)

TAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTCGCGCAGTG  >  minE/1680705‑1680820

tAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGtt                                               >  1:3233456/1‑71 (MQ=255)

tAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGtt                                               >  1:902787/1‑71 (MQ=255)

tAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGtt                                               >  1:3403718/1‑71 (MQ=255)

tAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGtt                                               >  1:1636039/1‑71 (MQ=255)

tAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGtt                                               >  1:2149400/1‑71 (MQ=255)

tAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGtt                                               >  1:4209699/1‑71 (MQ=255)

tAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGCTCAGCAAAGTGCACTGCTCATCGGGTAGCAGtt                                               >  1:4114503/1‑71 (MQ=255)

  gCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTa                                             <  1:2596930/71‑1 (MQ=255)

  gCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTa                                             <  1:57199/71‑1 (MQ=255)

  gCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTa                                             <  1:2124379/71‑1 (MQ=255)

     aGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTAttt                                          <  1:3010056/71‑1 (MQ=255)

     aGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTAttt                                          <  1:4016543/71‑1 (MQ=255)

     aGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTAttt                                          <  1:4108574/71‑1 (MQ=255)

     aGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTAttt                                          <  1:2055748/71‑1 (MQ=255)

     aGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTAttt                                          <  1:1805934/71‑1 (MQ=255)

     aGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTAttt                                          <  1:486663/71‑1 (MQ=255)

           cACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTttcttc                                     >  1:3553567/1‑70 (MQ=255)

           cACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTttcttc                                     >  1:1039213/1‑70 (MQ=255)

           cACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTttcttc                                     >  1:3374732/1‑70 (MQ=255)

           cACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTttcttc                                     >  1:3440843/1‑70 (MQ=255)

           cACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTttcttc                                     >  1:2761436/1‑70 (MQ=255)

              cAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAg                                  >  1:1307569/1‑70 (MQ=255)

              cAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAg                                  >  1:65010/1‑70 (MQ=255)

              cAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAg                                  >  1:2613466/1‑70 (MQ=255)

              cAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAg                                  >  1:459953/1‑70 (MQ=255)

              cAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAg                                  >  1:4314045/1‑70 (MQ=255)

                aaCGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGc                                 >  1:377219/1‑69 (MQ=255)

                aaCGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGc                                 >  1:4181124/1‑69 (MQ=255)

                aaCGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGc                                 >  1:4176415/1‑69 (MQ=255)

                aaCGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGc                                 >  1:886061/1‑69 (MQ=255)

                aaCGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGc                                 >  1:4252706/1‑69 (MQ=255)

                aaCGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGc                                 >  1:92926/1‑69 (MQ=255)

                aaCGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGc                                 >  1:2991635/1‑69 (MQ=255)

                aaCGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGc                                 >  1:1259548/1‑69 (MQ=255)

                aaCGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGc                                 >  1:165379/1‑69 (MQ=255)

                 aCGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAg                              <  1:4040838/71‑1 (MQ=255)

                              aaGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAAcg                 <  1:2871858/71‑1 (MQ=255)

                              aaGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAAcg                 <  1:106268/71‑1 (MQ=255)

                              aaGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAAcg                 <  1:3501337/71‑1 (MQ=255)

                              aaGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAAcg                 <  1:1631916/71‑1 (MQ=255)

                              aaGTGTGTTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAAcg                 <  1:1637372/71‑1 (MQ=255)

                                   tgtTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTAtttt                                         >  1:2204498/1‑42 (MQ=255)

                                   tgtTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTAtttt                                         >  1:492370/1‑42 (MQ=255)

                                   tgtTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTAtttt                                         >  1:4266609/1‑42 (MQ=255)

                                   tgtTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTAtttt                                         >  1:1858826/1‑42 (MQ=255)

                                   tgtTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTAtttt                                         >  1:2228688/1‑42 (MQ=255)

                                   tgtTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTAtttt                                         >  1:2330488/1‑42 (MQ=255)

                                   tgtTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTAtttt                                         >  1:286510/1‑42 (MQ=255)

                                   tgtTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTAtttt                                         >  1:394786/1‑42 (MQ=255)

                                   tgtTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTAtttt                                         >  1:3607547/1‑42 (MQ=255)

                                   tgtTCAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTAtttt                                         >  1:3735165/1‑42 (MQ=255)

                                       cAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTcg         <  1:2438836/70‑1 (MQ=255)

                                       cAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTcg         <  1:107560/70‑1 (MQ=255)

                                       cAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTcg         <  1:664294/70‑1 (MQ=255)

                                       cAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTcg         <  1:1537869/70‑1 (MQ=255)

                                       cAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTcg         <  1:2554858/70‑1 (MQ=255)

                                       cAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTcg         <  1:2524624/70‑1 (MQ=255)

                                       cAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTcg         <  1:1957463/70‑1 (MQ=255)

                                       cAGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTcg         <  1:4031365/70‑1 (MQ=255)

                                        aGCAAAGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTcg         <  1:2797029/69‑1 (MQ=255)

                                           aaaGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTCGCGCAg    <  1:165434/71‑1 (MQ=255)

                                             aGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTCGCGCAGTg  <  1:3875729/71‑1 (MQ=255)

                                             aGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTCGCGCAGTg  <  1:494573/71‑1 (MQ=255)

                                             aGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTCGCGCAGTg  <  1:3047851/71‑1 (MQ=255)

                                             aGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTCGCGCAGTg  <  1:1045195/71‑1 (MQ=255)

                                             aGTGCACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAAAGCATTAACGCGTTGTCGCGCAGTg  <  1:693673/71‑1 (MQ=255)

TAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAGAGCATTAACGCGTTGTCGCGCAGTG  >  minE/1680705‑1680820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`