Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,700,462	A→G	L24P (CTA→CCA)	hcaT ←	predicted 3‑phenylpropionic transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,700,462	0	A	G	100.0%	246.7 / NA	66	L24P (CTA→CCA)	hcaT	predicted 3‑phenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/34);  total (32/34)

ACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCAAAAC  >  minE/1700403‑1700529                                                            |                                                                    aCAGGCCGATGGTTTCTGGCGTTATACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCg                                                          >  1:297310/1‑71 (MQ=255) aCAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCg                                                          >  1:2230643/1‑71 (MQ=255) aCAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCg                                                          >  1:582067/1‑71 (MQ=255) aCAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCg                                                          >  1:2263374/1‑71 (MQ=255) aCAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCg                                                          >  1:4352847/1‑71 (MQ=255) aCAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCg                                                          >  1:412212/1‑71 (MQ=255) aCAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCg                                                          >  1:1791694/1‑71 (MQ=255) aCAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCg                                                          >  1:3260156/1‑71 (MQ=255)  cAGGCCGATGGTTTCTGGCGTTCAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGt                                                         <  1:583999/71‑1 (MQ=255)  cAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGAGGCTCCAGAAAGGTGGAAAAATGCCGt                                                         <  1:896032/71‑1 (MQ=255)  cAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAACTGCCGt                                                         <  1:3530/71‑1 (MQ=255)  cAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGt                                                         <  1:3355538/71‑1 (MQ=255)  cAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGt                                                         <  1:965213/71‑1 (MQ=255)  cAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGt                                                         <  1:3087816/71‑1 (MQ=255)  cAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGt                                                         <  1:4224888/71‑1 (MQ=255)  cAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGt                                                         <  1:2761097/71‑1 (MQ=255)  cAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGt                                                         <  1:449778/71‑1 (MQ=255)  cAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGt                                                         <  1:1048871/71‑1 (MQ=255)   aGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTa                                                        <  1:2544883/71‑1 (MQ=255)   aGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTa                                                        <  1:3302844/71‑1 (MQ=255)   aGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTa                                                        <  1:154437/71‑1 (MQ=255)   aGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTa                                                        <  1:3576889/71‑1 (MQ=255)   aGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTa                                                        <  1:1811472/71‑1 (MQ=255)   aGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTa                                                        <  1:3740593/71‑1 (MQ=255)   aGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTa                                                        <  1:2324049/71‑1 (MQ=255)   aGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTa                                                        <  1:3760959/71‑1 (MQ=255)   aGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTa                                                        <  1:2156907/71‑1 (MQ=255)   aGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTa                                                        <  1:4101891/71‑1 (MQ=255)   aGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTa                                                        <  1:1497533/71‑1 (MQ=255)   aGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGGGGAAAAATGCCGTa                                                        <  1:2477406/71‑1 (MQ=255)   aGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTAAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTa                                                        <  1:1806468/71‑1 (MQ=255)             tttCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAg                                              <  1:3723224/71‑1 (MQ=255)              ttCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAg                                              >  1:3874962/1‑70 (MQ=255)                    cGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGa                                       <  1:1418790/71‑1 (MQ=255)                    cGTTAAACCAATACCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGa                                       <  1:1809076/71‑1 (MQ=255)                        aaaCCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGaaa                                     >  1:3833791/1‑69 (MQ=255)                        aaaCCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGaaa                                     >  1:310835/1‑69 (MQ=255)                        aaaCCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGaaa                                     >  1:3522094/1‑69 (MQ=255)                        aaaCCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGaaa                                     >  1:1686024/1‑69 (MQ=255)                        aaaCCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGaaa                                     >  1:198922/1‑69 (MQ=255)                        aaaCCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGaaa                                     >  1:1758412/1‑69 (MQ=255)                        aaaCCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGaaa                                     >  1:2620686/1‑69 (MQ=255)                        aaaCCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGaaa                                     >  1:2412057/1‑69 (MQ=255)                        aaaCCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGaaa                                     >  1:951156/1‑69 (MQ=255)                                          cAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAgcg                 >  1:3921457/1‑71 (MQ=255)                                          cAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAgcg                 >  1:3527650/1‑71 (MQ=255)                                          cAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAgcg                 >  1:1301761/1‑71 (MQ=255)                                              cGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg             <  1:1389462/71‑1 (MQ=255)                                               gCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTgg            <  1:3579947/71‑1 (MQ=255)                                               gCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTgg            <  1:1910869/71‑1 (MQ=255)                                               gCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTgg            <  1:1813383/71‑1 (MQ=255)                                               gCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTgg            <  1:2913526/71‑1 (MQ=255)                                               gCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTgg            <  1:1385693/71‑1 (MQ=255)                                               gCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTgg            <  1:3061634/71‑1 (MQ=255)                                                   cAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGAt          <  1:304984/69‑1 (MQ=255)                                                         ggTGGAAAAATGCCGTAACTAAAAAAGTATTTGAAATAGACGAGCGCCAACCAGcgcg               >  1:2031568/1‑58 (MQ=255)                                                         ggTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCaaaa   >  1:4802/1‑70 (MQ=255)                                                         ggTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCAAAAc  >  1:547718/1‑71 (MQ=255)                                                         ggTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCAAAAc  >  1:1328368/1‑71 (MQ=255)                                                         ggTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCAAAAc  >  1:1056504/1‑71 (MQ=255)                                                         ggTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCAAAAc  >  1:1543952/1‑71 (MQ=255)                                                         ggTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCAAAAc  >  1:3504074/1‑71 (MQ=255)                                                         ggTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCAAAAc  >  1:3448418/1‑71 (MQ=255)                                                         ggTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCAAAAc  >  1:2803913/1‑71 (MQ=255)                                                         ggTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCAAAAc  >  1:2280115/1‑71 (MQ=255)                                                         ggTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCAAAAc  >  1:2103241/1‑71 (MQ=255)                                                            |                                                                    ACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCAAAAC  >  minE/1700403‑1700529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑