BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,777,713	T→C	N105D (AAT→GAT)	trmD ←	tRNA (guanine‑1‑)‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,777,713	0	T	C	100.0%	405.0 / NA	107	N105D (AAT→GAT)	trmD	tRNA (guanine‑1‑)‑methyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/83); total (24/83)

Only 100 of 107 total aligned reads displayed.

ATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAT  >  minE/1777658‑1777773

aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:898234/71‑1 (MQ=255)

aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:1858624/71‑1 (MQ=255)

aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:2041318/71‑1 (MQ=255)

aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:2080650/71‑1 (MQ=255)

aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:1760662/71‑1 (MQ=255)

aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:2085258/71‑1 (MQ=255)

aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:1700298/71‑1 (MQ=255)

aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:28105/71‑1 (MQ=255)

aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:3310686/71‑1 (MQ=255)

aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:3529932/71‑1 (MQ=255)

aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:1409267/71‑1 (MQ=255)

aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:3863921/71‑1 (MQ=255)

aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:3635530/71‑1 (MQ=255)

aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:457011/71‑1 (MQ=255)

aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:1180963/71‑1 (MQ=255)

aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:1330343/71‑1 (MQ=255)

aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:4028495/71‑1 (MQ=255)

 tCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:1400040/70‑1 (MQ=255)

                  ccTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:4272235/53‑1 (MQ=255)

                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:2694513/69‑1 (MQ=255)

                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:2225840/69‑1 (MQ=255)

                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:2344355/69‑1 (MQ=255)

                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:3927871/69‑1 (MQ=255)

                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:2474816/69‑1 (MQ=255)

                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:2658076/69‑1 (MQ=255)

                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:3023921/69‑1 (MQ=255)

                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:3037784/69‑1 (MQ=255)

                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:3138691/69‑1 (MQ=255)

                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:1782238/69‑1 (MQ=255)

                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:1421702/69‑1 (MQ=255)

                      cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:1228622/69‑1 (MQ=255)

                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:868296/59‑1 (MQ=255)

                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:3156119/59‑1 (MQ=255)

                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:1628759/59‑1 (MQ=255)

                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:276014/59‑1 (MQ=255)

                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:110947/59‑1 (MQ=255)

                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:2674609/59‑1 (MQ=255)

                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:256835/59‑1 (MQ=255)

                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:1663208/59‑1 (MQ=255)

                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:1400094/59‑1 (MQ=255)

                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:239776/59‑1 (MQ=255)

                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:401044/59‑1 (MQ=255)

                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:1187419/59‑1 (MQ=255)

                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:646706/59‑1 (MQ=255)

                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:660364/59‑1 (MQ=255)

                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:672101/59‑1 (MQ=255)

                              ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCACGCTTGATCAAg                             <  1:1518466/59‑1 (MQ=255)

                              ccGCACACCACAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:1551345/59‑1 (MQ=255)

                               cGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                             <  1:3476892/58‑1 (MQ=255)

                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:3666287/1‑59 (MQ=255)

                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:4107/1‑59 (MQ=255)

                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:1143526/1‑59 (MQ=255)

                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:636982/1‑59 (MQ=255)

                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:3422433/1‑59 (MQ=255)

                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:712474/1‑59 (MQ=255)

                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:1122794/1‑59 (MQ=255)

                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:295411/1‑59 (MQ=255)

                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:192687/1‑59 (MQ=255)

                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:161891/1‑59 (MQ=255)

                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:2783312/1‑59 (MQ=255)

                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:209410/1‑59 (MQ=255)

                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:1651489/1‑59 (MQ=255)

                                gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           >  1:252869/1‑59 (MQ=255)

                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:3762359/71‑1 (MQ=255)

                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:2273857/71‑1 (MQ=255)

                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:1371703/71‑1 (MQ=255)

                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:2272240/71‑1 (MQ=255)

                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:1200738/71‑1 (MQ=255)

                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:52682/71‑1 (MQ=255)

                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:596239/71‑1 (MQ=255)

                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:1803408/71‑1 (MQ=255)

                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:847399/71‑1 (MQ=255)

                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:1817239/71‑1 (MQ=255)

                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:3155101/71‑1 (MQ=255)

                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:291648/71‑1 (MQ=255)

                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:3513140/71‑1 (MQ=255)

                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:3491250/71‑1 (MQ=255)

                                   cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            <  1:3379874/71‑1 (MQ=255)

                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:3727918/71‑1 (MQ=255)

                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:3127976/71‑1 (MQ=255)

                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:1961501/71‑1 (MQ=255)

                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:2011368/71‑1 (MQ=255)

                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:609093/71‑1 (MQ=255)

                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:3280330/71‑1 (MQ=255)

                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:1752509/71‑1 (MQ=255)

                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:4282727/71‑1 (MQ=255)

                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:4123528/71‑1 (MQ=255)

                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:1731406/71‑1 (MQ=255)

                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:2497518/71‑1 (MQ=255)

                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:2433887/71‑1 (MQ=255)

                                    acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           <  1:3732774/71‑1 (MQ=255)

                                     ccAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggtg            >  1:3220197/1‑69 (MQ=255)

                                     ccAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           >  1:402399/1‑70 (MQ=255)

                                     ccAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           >  1:635729/1‑70 (MQ=255)

                                     ccAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           >  1:2048195/1‑70 (MQ=255)

                                     ccAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           >  1:1728457/1‑70 (MQ=255)

                                     ccAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           >  1:1899962/1‑70 (MQ=255)

                                     ccAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa           >  1:1588094/1‑70 (MQ=255)

                                        gAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                           <  1:3516269/51‑1 (MQ=255)

                                               tttttGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:623848/69‑1 (MQ=255)

ATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAT  >  minE/1777658‑1777773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`