Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	195,616	C→T	L71L (CTG→CTA)	yaeF ←	predicted lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	195,616	0	C	T	100.0%	109.0 / NA	33	L71L (CTG→CTA)	yaeF	predicted lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/15);  total (18/15)

GTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTT  >  minE/195566‑195683                                                   |                                                                    gTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTgag                                                 <  1:1197271/71‑1 (MQ=255) gTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTgag                                                 <  1:3052758/71‑1 (MQ=255) gTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTgag                                                 <  1:3858893/71‑1 (MQ=255) gTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTgag                                                 <  1:1593890/71‑1 (MQ=255)   gCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt                                                    >  1:3683667/1‑66 (MQ=255)   gCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt                                                    >  1:3817488/1‑66 (MQ=255)     tGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt                                                    <  1:3148794/64‑1 (MQ=255)     tGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt                                                    <  1:1671656/64‑1 (MQ=255)     tGAAGACCCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt                                                    <  1:2285346/64‑1 (MQ=255)         gACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTg                                         >  1:3230297/1‑71 (MQ=255)           cgcgGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGcc                                       >  1:835103/1‑71 (MQ=255)           cgcgGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGcc                                       >  1:2099197/1‑71 (MQ=255)           cgcgGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGcc                                       >  1:61628/1‑71 (MQ=255)           cgcgGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGcc                                       >  1:2679144/1‑71 (MQ=255)            gcgGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt                                                    <  1:2204509/57‑1 (MQ=255)            gcgGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTgag                                                 <  1:1526368/60‑1 (MQ=255)              ggATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGtt                                    <  1:3421322/71‑1 (MQ=255)                        tGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTa                          <  1:1387332/71‑1 (MQ=255)                        tGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTa                          <  1:1390618/71‑1 (MQ=255)                            gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga                      >  1:3353586/1‑71 (MQ=255)                            gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga                      >  1:1368376/1‑71 (MQ=255)                            gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga                      >  1:3094667/1‑71 (MQ=255)                            gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga                      >  1:2429766/1‑71 (MQ=255)                            gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga                      >  1:2369307/1‑71 (MQ=255)                            gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga                      >  1:2195045/1‑71 (MQ=255)                                                gaATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCttt  <  1:3191787/71‑1 (MQ=255)                                                gaATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCttt  >  1:3014719/1‑71 (MQ=255)                                                gaATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCttt  <  1:2271000/71‑1 (MQ=255)                                                gaATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCttt  >  1:2204358/1‑71 (MQ=255)                                                gaATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCttt  <  1:582216/71‑1 (MQ=255)                                                gaATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCttt  >  1:1701979/1‑71 (MQ=255)                                                gaATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCtt   >  1:575201/1‑70 (MQ=255)                                                gaATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCtt   >  1:89600/1‑70 (MQ=255)                                                   |                                                                    GTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTT  >  minE/195566‑195683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑