Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,784,184 T→C I223T (ATA→ACA)  yfjB → NAD kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,784,1840TC100.0% 999.3 / NA 255I223T (ATA→ACA) yfjBNAD kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (143/112);  total (143/112)

Only 100 of 255 total aligned reads displayed.

CATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGC  >  minE/1784146‑1784248
                                      |                                                                
cATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGttttt                                  <  1:2387743/71‑1 (MQ=255)
cATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGttttt                                  <  1:1630232/71‑1 (MQ=255)
cATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGttttt                                  <  1:1534881/71‑1 (MQ=255)
cATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGttttt                                  <  1:1934099/71‑1 (MQ=255)
cATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGttttt                                  <  1:635973/71‑1 (MQ=255)
cATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGttttt                                  <  1:4310120/71‑1 (MQ=255)
  tGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGa                                                 <  1:2706468/54‑1 (MQ=255)
  tGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGa                                                 <  1:118858/54‑1 (MQ=255)
  tGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGa                                                 <  1:3556988/54‑1 (MQ=255)
  tGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGa                                                 <  1:2483389/54‑1 (MQ=255)
  tGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGa                                                 <  1:1093469/54‑1 (MQ=255)
  tGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGa                                                 <  1:4252639/54‑1 (MQ=255)
  tGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGa                                                 <  1:3327125/54‑1 (MQ=255)
   gTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGa                                                 <  1:1289703/53‑1 (MQ=255)
    ttCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGa                                                 >  1:1703613/1‑52 (MQ=255)
    ttCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATc                                               >  1:3325712/1‑54 (MQ=255)
    ttCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATc                                               >  1:2245531/1‑54 (MQ=255)
    ttCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATc                                               >  1:1574148/1‑54 (MQ=255)
    ttCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATc                                               >  1:1502338/1‑54 (MQ=255)
    ttCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATc                                               >  1:1221046/1‑54 (MQ=255)
    ttCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATc                                               >  1:3661457/1‑54 (MQ=255)
    ttCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATc                                               >  1:89399/1‑54 (MQ=255)
    ttCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGc                               >  1:1405367/1‑70 (MQ=255)
    ttCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCa                              >  1:3941279/1‑71 (MQ=255)
    ttCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCa                              >  1:3903446/1‑71 (MQ=255)
    ttCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCa                              >  1:3171295/1‑71 (MQ=255)
    ttCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCa                              >  1:2980684/1‑71 (MQ=255)
    ttCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCa                              >  1:1046000/1‑71 (MQ=255)
    ttCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCa                              >  1:2755377/1‑71 (MQ=255)
    ttCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCa                              >  1:3601662/1‑71 (MQ=255)
    ttCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCa                              >  1:2429076/1‑71 (MQ=255)
    ttCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCa                              >  1:4370234/1‑71 (MQ=255)
    ttCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCa                              >  1:1899208/1‑71 (MQ=255)
    ttCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCa                              >  1:825985/1‑71 (MQ=255)
    ttCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCa                              >  1:588473/1‑71 (MQ=255)
    ttCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCa                              >  1:2116754/1‑71 (MQ=255)
    ttCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCa                              >  1:1610/1‑71 (MQ=255)
    ttCCCGCATACGTTCTCAGCACGACCACTGGTCACAAACagcagcagc                                                     >  1:500606/1‑48 (MQ=39)
    ttCCCACATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCa                              >  1:4167267/1‑71 (MQ=255)
      cccGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATc                            >  1:253153/1‑71 (MQ=255)
      cccGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATc                            >  1:2932512/1‑71 (MQ=255)
      cccGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATc                            >  1:690349/1‑71 (MQ=255)
      cccGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATc                            >  1:1748242/1‑71 (MQ=255)
            tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGc                               <  1:1100488/62‑1 (MQ=255)
            tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGc                               <  1:398106/62‑1 (MQ=255)
            tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGc                               <  1:3838269/62‑1 (MQ=255)
            tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGc                               <  1:849127/62‑1 (MQ=255)
            tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGc                               <  1:228606/62‑1 (MQ=255)
            tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGc                               <  1:1807887/62‑1 (MQ=255)
            tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGc                          <  1:478174/67‑1 (MQ=255)
            tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGc                          <  1:2916639/67‑1 (MQ=255)
            tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGc                          <  1:19715/67‑1 (MQ=255)
            tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGc                          <  1:2009893/67‑1 (MQ=255)
            tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGc                          <  1:349130/67‑1 (MQ=255)
            tACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGc                          <  1:377450/67‑1 (MQ=255)
             aCGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGc                               <  1:3456491/61‑1 (MQ=255)
              cGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACg                                                  >  1:4197440/1‑41 (MQ=255)
              cGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTg                                         >  1:2334013/1‑50 (MQ=255)
              cGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                             >  1:731064/1‑62 (MQ=255)
              cGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                             >  1:2784769/1‑62 (MQ=255)
              cGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCg                        >  1:3096233/1‑67 (MQ=255)
              cGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCg                        >  1:465315/1‑67 (MQ=255)
              cGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCg                        >  1:3259313/1‑67 (MQ=255)
              cGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCg                        >  1:4286448/1‑67 (MQ=255)
              cGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCg                        >  1:2606320/1‑67 (MQ=255)
              cGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCg                        >  1:1411644/1‑67 (MQ=255)
                  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCTCATCGCCGTAACGAc                 <  1:2693982/70‑1 (MQ=255)
                  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:3446369/70‑1 (MQ=255)
                  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:1337082/70‑1 (MQ=255)
                  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:2352371/70‑1 (MQ=255)
                   tCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGttttt                                  <  1:2057529/52‑1 (MQ=255)
                   tCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:3048079/69‑1 (MQ=255)
                         cGACCACTGGTCACAAA‑‑‑CAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATc        <  1:4094943/69‑1 (MQ=255)
                          gACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATc        <  1:2880767/71‑1 (MQ=255)
                          gACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATc        <  1:3827037/71‑1 (MQ=255)
                          gACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATc        <  1:3746710/71‑1 (MQ=255)
                          gACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATc        <  1:3189939/71‑1 (MQ=255)
                          gACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATc        <  1:2083846/71‑1 (MQ=255)
                          gACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATc        <  1:2212861/71‑1 (MQ=255)
                          gACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATc        <  1:2829614/71‑1 (MQ=255)
                                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATc                            >  1:2378267/1‑45 (MQ=255)
                                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:3103502/1‑55 (MQ=255)
                                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGaaa          >  1:1080670/1‑63 (MQ=255)
                                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGaaa          >  1:1640992/1‑63 (MQ=255)
                                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGaaa          >  1:168540/1‑63 (MQ=255)
                                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGaaa          >  1:1761532/1‑63 (MQ=255)
                                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGaaa          >  1:1941935/1‑63 (MQ=255)
                                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGaaa          >  1:2171393/1‑63 (MQ=255)
                                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGaaa          >  1:2641379/1‑63 (MQ=255)
                                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGaaa          >  1:2969730/1‑63 (MQ=255)
                                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGaaa          >  1:3118511/1‑63 (MQ=255)
                                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGaaa          >  1:3967975/1‑63 (MQ=255)
                                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  >  1:2019492/1‑71 (MQ=255)
                                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:2845099/69‑1 (MQ=255)
                                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:66131/69‑1 (MQ=255)
                                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:3392966/69‑1 (MQ=255)
                                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:1734195/69‑1 (MQ=255)
                                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:3530927/69‑1 (MQ=255)
                                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:3877553/69‑1 (MQ=255)
                                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:3789186/69‑1 (MQ=255)
                                      |                                                                
CATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGC  >  minE/1784146‑1784248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑