BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,833,531	C→T	R92C (CGC→TGC)	ygbL →	predicted class II aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,833,531	0	C	T	100.0%	396.7 / NA	116	R92C (CGC→TGC)	ygbL	predicted class II aldolase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (81/35); total (81/35)

Only 100 of 116 total aligned reads displayed.

TGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGG  >  minE/1833465‑1833593

tGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTg                                                            >  1:2250019/1‑71 (MQ=255)

tGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTg                                                            >  1:1690368/1‑71 (MQ=255)

        tggtgACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCgg                                                    >  1:2674255/1‑71 (MQ=255)

        tggtgACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCgg                                                    >  1:1465410/1‑71 (MQ=255)

        tggtgACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCgg                                                    >  1:3158662/1‑71 (MQ=255)

          gtgACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtg                                                  >  1:1463057/1‑71 (MQ=255)

          gtgACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtg                                                  >  1:761491/1‑71 (MQ=255)

          gtgACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtg                                                  >  1:2161135/1‑71 (MQ=255)

          gtgACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtg                                                  >  1:2526156/1‑71 (MQ=255)

          gtgACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtg                                                  >  1:1158441/1‑71 (MQ=255)

          gtgACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtg                                                  >  1:2743414/1‑71 (MQ=255)

          gtgACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtg                                                  >  1:3790802/1‑71 (MQ=255)

             aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               <  1:2362948/71‑1 (MQ=255)

             aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               <  1:299025/71‑1 (MQ=255)

             aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               <  1:2362826/71‑1 (MQ=255)

             aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               <  1:4263748/71‑1 (MQ=255)

             aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               <  1:644038/71‑1 (MQ=255)

             aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               <  1:326320/71‑1 (MQ=255)

             aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               <  1:2666695/71‑1 (MQ=255)

             aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               <  1:1808216/71‑1 (MQ=255)

             aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               <  1:3728565/71‑1 (MQ=255)

             aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               <  1:3449305/71‑1 (MQ=255)

             aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               <  1:3507537/71‑1 (MQ=255)

             aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               <  1:831548/71‑1 (MQ=255)

             aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               <  1:3558802/71‑1 (MQ=255)

             aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               <  1:767439/71‑1 (MQ=255)

             aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               <  1:862726/71‑1 (MQ=255)

             aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               <  1:1343227/71‑1 (MQ=255)

             aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               <  1:3908975/71‑1 (MQ=255)

             aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               <  1:3611106/71‑1 (MQ=255)

             aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               <  1:3627994/71‑1 (MQ=255)

              cAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               <  1:4108705/70‑1 (MQ=255)

              cAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               <  1:268675/70‑1 (MQ=255)

              cAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               <  1:347353/70‑1 (MQ=255)

              cAAACCCTCGAAAGAGGTGCGCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               <  1:2336558/70‑1 (MQ=255)

               aaaCCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               >  1:3589012/1‑69 (MQ=255)

               aaaCCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               >  1:3319415/1‑69 (MQ=255)

               aaaCCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               >  1:3373167/1‑69 (MQ=255)

               aaaCCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               >  1:906501/1‑69 (MQ=255)

               aaaCCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               >  1:1300020/1‑69 (MQ=255)

               aaaCCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               >  1:2516308/1‑69 (MQ=255)

               aaaCCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               >  1:1758911/1‑69 (MQ=255)

               aaaCCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                               >  1:810029/1‑69 (MQ=255)

               aaaCCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCa                                             >  1:3754309/1‑71 (MQ=255)

                  cccTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCAtt                                           >  1:1130266/1‑70 (MQ=255)

                  cccTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCAtt                                           >  1:504927/1‑70 (MQ=255)

                  cccTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCAtt                                           >  1:1134032/1‑70 (MQ=255)

                  cccTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCAtt                                           >  1:37096/1‑70 (MQ=255)

                  cccTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCAtt                                           >  1:876625/1‑70 (MQ=255)

                  cccTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCAtt                                           >  1:1212120/1‑70 (MQ=255)

                   ccTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTg                                         <  1:3080166/71‑1 (MQ=255)

                   ccTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTg                                         <  1:1676939/71‑1 (MQ=255)

                   ccTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTg                                         <  1:21594/71‑1 (MQ=255)

                   ccTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTg                                         <  1:2182962/71‑1 (MQ=255)

                       gAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTgcac                                      <  1:2846972/70‑1 (MQ=255)

                       gAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTgcac                                      <  1:487873/70‑1 (MQ=255)

                        aaaGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTgcacag                                    <  1:2178200/71‑1 (MQ=255)

                        aaaGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTgcacag                                    <  1:3101594/71‑1 (MQ=255)

                        aaaGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTgcacag                                    <  1:3106627/71‑1 (MQ=255)

                              gTGCTCTTTCATCTGGCGCTGTATCGAAACAATCCGTGCTGTaa                                                         >  1:4044307/1‑44 (MQ=38)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTcc              >  1:330796/1‑60 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:855581/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:4339507/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:4021763/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:550994/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:685877/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:3988617/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:3935066/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:4339848/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:4347985/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:468246/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:411825/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:3244933/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:3835813/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:2833880/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:1105933/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:1138000/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:1479570/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:1519695/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:162259/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:2110365/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:2139569/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:2252663/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:2327973/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:2752939/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:3809446/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:2920469/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:3040898/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:3085871/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:106134/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:3283039/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:3321689/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:3499034/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:3605965/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:3697720/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:3707823/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:3716837/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCCCTTTCCTGCCTGCAAgg   >  1:3136002/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAG‑ACATGGTCGACGGCGCTTTCCTGCCTGCAAggg  >  1:2304887/1‑71 (MQ=255)

                                                         aacaaTCCGTGCTGTAAAGAGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg   >  1:1791254/1‑71 (MQ=255)

TGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGG  >  minE/1833465‑1833593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`