Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,867,615	G→A	T429I (ACC→ATC)	ygcU ←	predicted FAD containing dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,867,615	0	G	A	100.0%	291.4 / NA	84	T429I (ACC→ATC)	ygcU	predicted FAD containing dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/57);  total (27/57)

CTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCTTA  >  minE/1867552‑1867677                                                                |                                                               ccccAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATttctt                                                          <  1:211140/68‑1 (MQ=255) cTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATttctt                                                          <  1:683689/70‑1 (MQ=255) cTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATttctt                                                          <  1:4202393/70‑1 (MQ=255) cTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATttctt                                                          <  1:4221264/70‑1 (MQ=255) cTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATttctt                                                          <  1:2540312/70‑1 (MQ=255) cTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATttctt                                                          <  1:1250211/70‑1 (MQ=255) cTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATttctt                                                          <  1:1117003/70‑1 (MQ=255) cTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATttctt                                                          <  1:3602182/70‑1 (MQ=255)    cAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTgaca                                                      <  1:1734129/71‑1 (MQ=255)    cAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTcaca                                                      <  1:3895695/71‑1 (MQ=255)    cAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTcaca                                                      <  1:3946133/71‑1 (MQ=255)    cAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTcaca                                                      <  1:2183523/71‑1 (MQ=255)        gAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgat                                                   >  1:4370056/1‑70 (MQ=255)        gAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgat                                                   >  1:3632501/1‑70 (MQ=255)         aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatga                                                 >  1:2622637/1‑71 (MQ=255)         aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatga                                                 >  1:3683799/1‑71 (MQ=255)         aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatga                                                 >  1:3861562/1‑71 (MQ=255)         aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatga                                                 >  1:3068747/1‑71 (MQ=255)         aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatga                                                 <  1:2160176/71‑1 (MQ=255)         aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatga                                                 <  1:4310311/71‑1 (MQ=255)         aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatga                                                 >  1:631770/1‑71 (MQ=255)         aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatga                                                 >  1:884544/1‑71 (MQ=255)         aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatga                                                 >  1:714216/1‑71 (MQ=255)         aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatga                                                 >  1:776633/1‑71 (MQ=255)         aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatga                                                 <  1:1503830/71‑1 (MQ=255)         aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCAAAgatga                                                 <  1:3676533/71‑1 (MQ=255)         aaCGCAATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatga                                                 >  1:1067825/1‑71 (MQ=255)          aCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatga                                                 <  1:3459967/70‑1 (MQ=255)                          tACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATtg                                <  1:421579/71‑1 (MQ=255)                          tACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATtg                                <  1:932234/71‑1 (MQ=255)                          tACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATtg                                <  1:3832005/71‑1 (MQ=255)                          tACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATtg                                <  1:2330032/71‑1 (MQ=255)                          tACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATtg                                <  1:2050725/71‑1 (MQ=255)                            ccGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATtgt                               <  1:1672579/70‑1 (MQ=255)                            ccGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATtgt                               <  1:226366/70‑1 (MQ=255)                            ccGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATtgt                               <  1:481150/70‑1 (MQ=255)                             cGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATtgt                               <  1:3795455/69‑1 (MQ=255)                             cGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTgg                             <  1:3178889/71‑1 (MQ=255)                             cGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTgg                             <  1:1537602/71‑1 (MQ=255)                              gtggtggtgCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATtgt                               <  1:3172808/68‑1 (MQ=255)                                 gtggtgCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACt                         >  1:3056544/1‑71 (MQ=255)                                       cACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTg                       <  1:4263911/67‑1 (MQ=255)                                       cACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTg                       <  1:1892229/67‑1 (MQ=255)                                       cACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTg                       <  1:1800818/67‑1 (MQ=255)                                       cACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTg                       <  1:2723872/67‑1 (MQ=255)                                       cACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTg                       <  1:2504972/67‑1 (MQ=255)                                       cACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTg                       <  1:4088288/67‑1 (MQ=255)                                       cACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTg                       <  1:592830/67‑1 (MQ=255)                                       cACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTg                       <  1:170501/67‑1 (MQ=255)                                       cACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTg                       <  1:675149/67‑1 (MQ=255)                                       cACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTg                       <  1:710412/67‑1 (MQ=255)                                       cACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGa                   <  1:3444225/71‑1 (MQ=255)                                        aCCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTg                       <  1:4144717/66‑1 (MQ=255)                                         ccATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGa                                        >  1:1297595/1‑48 (MQ=255)                                         ccATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGAtt                 >  1:1762607/1‑71 (MQ=255)                                         ccATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGAtt                 >  1:180456/1‑71 (MQ=255)                                         ccATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGAtt                 >  1:445425/1‑71 (MQ=255)                                         ccATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGAtt                 >  1:3973660/1‑71 (MQ=255)                                         ccATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGAtt                 >  1:509111/1‑71 (MQ=255)                                         ccATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGAtt                 >  1:961670/1‑71 (MQ=255)                                         ccATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGAtt                 >  1:3917333/1‑71 (MQ=255)                                         ccATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGAtt                 >  1:3157259/1‑71 (MQ=255)                                         ccATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGAtt                 >  1:2197042/1‑71 (MQ=255)                                         ccATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGAtt                 >  1:368275/1‑71 (MQ=255)                                         ccATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGAtt                 >  1:2583360/1‑71 (MQ=255)                                         ccATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTCTTGAGCGGATAGTGGTACTTGTCGa                   >  1:2659739/1‑69 (MQ=255)                                           aTCGAACCGCCTAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTg                       <  1:1170347/63‑1 (MQ=255)                                            tCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTcc              >  1:1193468/1‑71 (MQ=255)                                            tCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTcc              >  1:3345041/1‑71 (MQ=255)                                                aCCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGTATTGTGGTACTTg                       <  1:1041978/58‑1 (MQ=255)                                                      gAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCt    <  1:1865443/71‑1 (MQ=255)                                                      gAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCt    <  1:2633777/71‑1 (MQ=255)                                                      gAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCt    <  1:2756540/71‑1 (MQ=255)                                                      gAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCt    <  1:3542533/71‑1 (MQ=255)                                                      gAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCt    <  1:2381467/71‑1 (MQ=255)                                                      gAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCt    <  1:1635084/71‑1 (MQ=255)                                                      gAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCt    <  1:3210413/71‑1 (MQ=255)                                                        ggCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCTTa  <  1:93567/71‑1 (MQ=255)                                                        ggCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCTTa  <  1:290159/71‑1 (MQ=255)                                                        ggCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCTTa  <  1:3234803/71‑1 (MQ=255)                                                        ggCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCTTa  <  1:3453473/71‑1 (MQ=255)                                                        ggCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCTTa  <  1:2882010/71‑1 (MQ=255)                                                        ggCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCTTa  <  1:2051703/71‑1 (MQ=255)                                                        ggCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCTTa  <  1:2526507/71‑1 (MQ=255)                                                                |                                                               CTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCTTA  >  minE/1867552‑1867677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑