Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,187,308	T→C	P183P (CCA→CCG)	nanT ←	sialic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,187,308	0	T	C	100.0%	152.9 / NA	42	P183P (CCA→CCG)	nanT	sialic acid transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/14);  total (28/14)

CGTGTTTCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTA  >  minE/2187244‑2187365                                                                 |                                                          cGTGTTTCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCaa                                                      <  1:1121165/70‑1 (MQ=255) cGTGTTTCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCaa                                                      <  1:913365/70‑1 (MQ=255) cGTGTTTCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCaa                                                      <  1:387020/70‑1 (MQ=255) cGTGTTTCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCaa                                                      <  1:3137323/70‑1 (MQ=255) cGTGTTTCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCaa                                                      <  1:2759597/70‑1 (MQ=255) cGTGTTTCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCaa                                                      <  1:1910830/70‑1 (MQ=255) cGTGTTTCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCaa                                                      <  1:1869001/70‑1 (MQ=255) cGTGTTTCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCaa                                                      <  1:110992/70‑1 (MQ=255) cGTGTTTCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCaa                                                      <  1:1297775/70‑1 (MQ=255) cGTGTCTCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCaa                                                      <  1:23308/70‑1 (MQ=255)     tttCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCaa                                                      <  1:293209/66‑1 (MQ=255)     tttCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCaa                                                      <  1:2875907/66‑1 (MQ=255)     tttCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCaa                                                      <  1:1572751/66‑1 (MQ=255)      ttCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCaa                                                      <  1:1824902/65‑1 (MQ=255)       tctcTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGcc                                                >  1:333965/1‑70 (MQ=255)       tctcTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGcc                                                >  1:2559111/1‑70 (MQ=255)       tctcTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGcc                                                >  1:1771545/1‑70 (MQ=255)       tctcTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGcc                                                >  1:2890839/1‑70 (MQ=255)       tctcTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGcc                                                >  1:3924011/1‑70 (MQ=255)       tctcTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGcc                                                >  1:2835522/1‑70 (MQ=255)                  tCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAGAACAg                                    >  1:3942109/1‑71 (MQ=255)                  tCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAGAACAg                                    >  1:4342584/1‑71 (MQ=255)                  tCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAGAACAg                                    >  1:178093/1‑71 (MQ=255)                      ccgcttccgGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAGAACAgcgc                                 >  1:4234240/1‑70 (MQ=255)                      ccgcttccgGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAGAACAGCGCa                                >  1:3223063/1‑71 (MQ=255)                      ccgcttccgGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAGAACAGCGCa                                >  1:4102427/1‑71 (MQ=255)                      ccgcttccgGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAGAACAGCGCa                                >  1:4279520/1‑71 (MQ=255)                      ccgcttccgGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAGAACAGCGCa                                >  1:3635271/1‑71 (MQ=255)                      ccgcttccgGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAGAACAGCGCa                                >  1:785648/1‑71 (MQ=255)                      ccgcttccgGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAGAACAGCGCa                                >  1:1600839/1‑71 (MQ=255)                      ccgcttccgGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAGAACAGCGCa                                >  1:2736295/1‑71 (MQ=255)                      ccgcttccgGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAGAACAGCGCa                                >  1:1899641/1‑71 (MQ=255)                               ggATGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAGAACAGCGCACGCCAGccc                       >  1:406225/1‑71 (MQ=255)                                  tGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCg                                               >  1:2791664/1‑44 (MQ=255)                                  tGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAGAACAGCGCACGCCAGccc                       >  1:454340/1‑68 (MQ=255)                                  tGTTTTTACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAGAACAGCGCACGCCAGccc                       >  1:2231566/1‑68 (MQ=255)                                      tttACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCg                >  1:3529444/1‑71 (MQ=255)                                      tttACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCg                >  1:1283046/1‑71 (MQ=255)                                      tttACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCg                >  1:1315597/1‑71 (MQ=255)                                      tttACGCAGCCAGAGAGCAAAGATGATCGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCg                >  1:2598348/1‑71 (MQ=255)                                                     agCAAAGATGATCGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCta  >  1:2586855/1‑70 (MQ=255)                                                     agCAAAGATGATCGACAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCta  >  1:3052113/1‑70 (MQ=255)                                                                 |                                                          CGTGTTTCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTA  >  minE/2187244‑2187365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑