Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,371,447 A→G F214L (TTT→CTT)  fre ← flavin reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,371,4470AG100.0% 653.8 / NA 168F214L (TTT→CTT) freflavin reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (59/109);  total (59/109)

Only 100 of 169 total aligned reads displayed.

AGATAAATGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGCTCTGC  >  minE/2371387‑2371507
                                                            |                                                            
aGATAAATGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATcgcg                                                     <  1:2217650/70‑1 (MQ=255)
aGATAAATGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATcgcg                                                     <  1:1566648/70‑1 (MQ=255)
aGATAAATGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATcgcg                                                     <  1:865570/70‑1 (MQ=255)
aGATAAATGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATcgcg                                                     <  1:1375763/70‑1 (MQ=255)
      aTGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAAtt                                              <  1:4032801/71‑1 (MQ=255)
        gCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAAtt                                              <  1:85056/69‑1 (MQ=255)
         cAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTc                                           <  1:1362150/71‑1 (MQ=255)
         cAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTc                                           <  1:2769830/71‑1 (MQ=255)
          aaaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTc                                           <  1:726782/70‑1 (MQ=255)
           aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                         <  1:82954/71‑1 (MQ=255)
           aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                         <  1:2446557/71‑1 (MQ=255)
           aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                         <  1:334400/71‑1 (MQ=255)
           aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                         <  1:4281300/71‑1 (MQ=255)
           aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                         <  1:3271330/71‑1 (MQ=255)
           aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                         <  1:1887493/71‑1 (MQ=255)
           aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                         <  1:3253510/71‑1 (MQ=255)
           aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                         <  1:671680/71‑1 (MQ=255)
           aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                         <  1:2855921/71‑1 (MQ=255)
           aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                         <  1:546704/71‑1 (MQ=255)
           aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                         <  1:537704/71‑1 (MQ=255)
           aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                         <  1:576964/71‑1 (MQ=255)
           aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                         <  1:2368158/71‑1 (MQ=255)
           aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                         <  1:970749/71‑1 (MQ=255)
           aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                         <  1:3556339/71‑1 (MQ=255)
           aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                         <  1:1805975/71‑1 (MQ=255)
           aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                         <  1:1800164/71‑1 (MQ=255)
           aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                         <  1:907796/71‑1 (MQ=255)
           aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                         <  1:3578160/71‑1 (MQ=255)
           aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                         <  1:424987/71‑1 (MQ=255)
           aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                         <  1:1442277/71‑1 (MQ=255)
                   gCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCAt                                      <  1:676678/66‑1 (MQ=255)
                   gCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCAt                                      <  1:271874/66‑1 (MQ=255)
                   gCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCAt                                      <  1:2515619/66‑1 (MQ=255)
                   gCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCAt                                      <  1:117040/66‑1 (MQ=255)
                   gCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCAt                                      <  1:3781650/66‑1 (MQ=255)
                   gCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCAt                                      <  1:1254239/66‑1 (MQ=255)
                   gCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCAt                                      <  1:1327337/66‑1 (MQ=255)
                   gCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCAt                                      <  1:2232780/66‑1 (MQ=255)
                   gCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCAt                                      <  1:171832/66‑1 (MQ=255)
                   gCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCAt                                      <  1:1918066/66‑1 (MQ=255)
                    ccAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCAt                                      <  1:3595333/65‑1 (MQ=255)
                      aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                              >  1:3859654/1‑71 (MQ=255)
                      aaaCAGGCGATCTTCCCGCGCATAACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                              >  1:1001353/1‑71 (MQ=255)
                         cAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                         <  1:362799/57‑1 (MQ=255)
                         cAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTc                           >  1:2801974/1‑71 (MQ=255)
                         cAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTc                           >  1:1555984/1‑71 (MQ=255)
                         cAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTc                           >  1:896909/1‑71 (MQ=255)
                         cAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTc                           >  1:2153746/1‑71 (MQ=255)
                         cAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTc                           >  1:3877694/1‑71 (MQ=255)
                         cAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTc                           >  1:3225125/1‑71 (MQ=255)
                         cAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTc                           >  1:314046/1‑71 (MQ=255)
                         cAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTc                           >  1:2714670/1‑71 (MQ=255)
                         cAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTc                           >  1:2355936/1‑71 (MQ=255)
                         cAGGCGATCTTCCCGCGCATAACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTc                           >  1:4337358/1‑71 (MQ=255)
                             cGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGc                       <  1:3690061/71‑1 (MQ=255)
                             cGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGc                       <  1:2592836/71‑1 (MQ=255)
                                 cTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAata                   >  1:4096612/1‑71 (MQ=255)
                                 cTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAata                   >  1:1078546/1‑71 (MQ=255)
                                 cTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAata                   >  1:3661461/1‑71 (MQ=255)
                                 cTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAata                   >  1:3985516/1‑71 (MQ=255)
                                 cTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAata                   >  1:1625754/1‑71 (MQ=255)
                                 cTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAata                   >  1:185128/1‑71 (MQ=255)
                                 cTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAata                   >  1:3507523/1‑71 (MQ=255)
                                 cTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAata                   >  1:4206855/1‑71 (MQ=255)
                                 cTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAata                   >  1:1915718/1‑71 (MQ=255)
                                 cTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAata                   >  1:2080488/1‑71 (MQ=255)
                                 cTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAata                   >  1:523297/1‑71 (MQ=255)
                                 cTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAata                   >  1:2951524/1‑71 (MQ=255)
                                 cTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAat                    >  1:1967335/1‑70 (MQ=255)
                                 cTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAat                    >  1:2266075/1‑70 (MQ=255)
                                        cgcATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTc                           <  1:4301259/56‑1 (MQ=255)
                                        cgcATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTc                           <  1:1020793/56‑1 (MQ=255)
                                        cgcATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTc                           <  1:3440186/56‑1 (MQ=255)
                                        cgcATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTc                           <  1:1434536/56‑1 (MQ=255)
                                        cgcATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTc                           <  1:1930495/56‑1 (MQ=255)
                                        cgcATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGatat            >  1:1484524/1‑71 (MQ=255)
                                        cgcATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGatat            >  1:4359708/1‑71 (MQ=255)
                                        cgcATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGatat            >  1:3948228/1‑71 (MQ=255)
                                          cATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCa          >  1:3305869/1‑71 (MQ=255)
                                                   cACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGCTCTGc  >  1:4065691/1‑70 (MQ=255)
                                                   cACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGCTCTGc  >  1:771508/1‑70 (MQ=255)
                                                   cACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGCTCTGc  >  1:1955323/1‑70 (MQ=255)
                                                     cTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGata             <  1:62086/57‑1 (MQ=255)
                                                     cTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGata             <  1:106148/57‑1 (MQ=255)
                                                     cTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGata             <  1:1107498/57‑1 (MQ=255)
                                                     cTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGata             <  1:1235798/57‑1 (MQ=255)
                                                     cTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGata             <  1:710794/57‑1 (MQ=255)
                                                     cTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGata             <  1:2295087/57‑1 (MQ=255)
                                                     cTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGata             <  1:2547273/57‑1 (MQ=255)
                                                     cTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGata             <  1:2905227/57‑1 (MQ=255)
                                                     cTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGata             <  1:3193054/57‑1 (MQ=255)
                                                     cTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGata             <  1:3335515/57‑1 (MQ=255)
                                                     cTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGata             <  1:3417036/57‑1 (MQ=255)
                                                        cAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGctct    <  1:2292649/63‑1 (MQ=255)
                                                        cAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGctct    <  1:2361661/63‑1 (MQ=255)
                                                        cAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGctct    <  1:724134/63‑1 (MQ=255)
                                                        cAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGctct    <  1:3900094/63‑1 (MQ=255)
                                                        cAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGctct    <  1:925964/63‑1 (MQ=255)
                                                        cAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGctct    <  1:3946749/63‑1 (MQ=255)
                                                         aaagCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGctct    <  1:3160422/62‑1 (MQ=255)
                                                            |                                                            
AGATAAATGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGCTCTGC  >  minE/2371387‑2371507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑