| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,450,298 | A→G | W78R (TGG→CGG) | yifE ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,450,298 | 0 | A | G | 99.6% | 884.3 / ‑4.9 | 228 | W78R (TGG→CGG) | yifE | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (110/117); minor base T (1/0); total (111/117) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
Only 100 of 251 total aligned reads displayed.
CTGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTC > minE/2450230‑2450364 | cTGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACcgca > 1:2151364/1‑71 (MQ=255)cTGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACcgca > 1:2430492/1‑71 (MQ=255)cTGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACcgca > 1:4291582/1‑71 (MQ=255)cTGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACcgca > 1:4199564/1‑71 (MQ=255)cTGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACcgca > 1:3079328/1‑71 (MQ=255)cTGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACcgca > 1:3955183/1‑71 (MQ=255)cTGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACcgca > 1:328879/1‑71 (MQ=255) tGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCgcac > 1:933082/1‑71 (MQ=255) tGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCgcac > 1:2603854/1‑71 (MQ=255) cGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCAcg < 1:19403/70‑1 (MQ=255) cGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCAcg < 1:772259/70‑1 (MQ=255) cGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCAcg < 1:874553/70‑1 (MQ=255) cGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGCACCGCAcg < 1:2581289/70‑1 (MQ=255) gTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGt > 1:3727607/1‑71 (MQ=255) gTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGt < 1:4033884/71‑1 (MQ=255) gTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGt > 1:1001411/1‑71 (MQ=255) gTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGt > 1:685579/1‑71 (MQ=255) gTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGt > 1:2017060/1‑71 (MQ=255) gTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGt < 1:2974384/71‑1 (MQ=255) gAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGt < 1:2495455/71‑1 (MQ=255) gAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGt < 1:2339550/71‑1 (MQ=255) aaGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCa > 1:1714021/1‑71 (MQ=255) ggTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTg < 1:2996790/71‑1 (MQ=255) ggTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTg < 1:1765232/71‑1 (MQ=255) ggTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTg < 1:2010597/71‑1 (MQ=255) ggTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTg < 1:2501936/71‑1 (MQ=255) ggTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTg < 1:3097402/71‑1 (MQ=255) ggTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTg < 1:3225157/71‑1 (MQ=255) ggTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTg < 1:2781747/71‑1 (MQ=255) ggTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTg < 1:1422028/71‑1 (MQ=255) gtgtGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTgg > 1:1097202/1‑71 (MQ=255) gtgtGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTgg > 1:3624260/1‑71 (MQ=255) gtgtGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTgg > 1:4307584/1‑71 (MQ=255) gtgtGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTg > 1:208078/1‑70 (MQ=255) tgtgAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCt > 1:3548957/1‑59 (MQ=255) tgtgAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGc > 1:2363859/1‑71 (MQ=255) tgtgAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGc > 1:1200830/1‑71 (MQ=255) tgtgAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGc > 1:2686990/1‑71 (MQ=255) tgtgAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGc > 1:2678202/1‑71 (MQ=255) tgtgAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGc > 1:819774/1‑71 (MQ=255) tgAAAACGTTTTGGACGCTTAATTCGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTg < 1:1566325/67‑1 (MQ=255) aaaaCGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCAc > 1:2461266/1‑71 (MQ=255) aaaaCGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCAc > 1:703993/1‑71 (MQ=255) aaCGTTTTGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGt < 1:2280128/71‑1 (MQ=255) ttttGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGc < 1:3669814/71‑1 (MQ=255) ttttGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGc < 1:4271415/71‑1 (MQ=255) ttttGGACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGc < 1:2862687/71‑1 (MQ=255) ggACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCAc < 1:3322970/70‑1 (MQ=255) ggACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCAc < 1:1801589/70‑1 (MQ=255) ggACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCAc < 1:3804050/70‑1 (MQ=255) ggACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCAc < 1:3283371/70‑1 (MQ=255) ggACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCAc < 1:1036360/70‑1 (MQ=255) ggACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCAc < 1:2544005/70‑1 (MQ=255) ggACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCAc < 1:1604015/70‑1 (MQ=255) ggACGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCAc < 1:2313498/70‑1 (MQ=255) cGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCa < 1:1579005/71‑1 (MQ=255) cGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCa < 1:3655408/71‑1 (MQ=255) cGCTTAATACGCGTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCa < 1:2229099/71‑1 (MQ=255) gcgTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACg < 1:3026347/71‑1 (MQ=255) gcgTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACg < 1:3904819/71‑1 (MQ=255) gcgTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACg < 1:4176663/71‑1 (MQ=255) gcgTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACg < 1:3370793/71‑1 (MQ=255) gcgTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACg < 1:2390805/71‑1 (MQ=255) gcgTCATATACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACg < 1:1168366/71‑1 (MQ=255) atatACTTGGACCGCACGCGTTCTGCTTCTGTCACt > 1:3682075/1‑36 (MQ=255) atatACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATa > 1:1342004/1‑58 (MQ=255) atatACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATa > 1:2292546/1‑58 (MQ=255) atatACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATa > 1:3754666/1‑58 (MQ=255) atatACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATa > 1:1195741/1‑58 (MQ=255) atatACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATa > 1:3059962/1‑58 (MQ=255) atatACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATa > 1:2631125/1‑58 (MQ=255) atatACTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATa > 1:3422933/1‑58 (MQ=255) taCTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTc < 1:2816387/44‑1 (MQ=255) taCTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTc < 1:409239/44‑1 (MQ=255) taCTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGttt > 1:1497118/1‑71 (MQ=255) taCTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGttt > 1:2651355/1‑71 (MQ=255) taCTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGtt > 1:3935818/1‑70 (MQ=255) taCTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGtt > 1:4022947/1‑70 (MQ=255) taCTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGtt > 1:2721568/1‑70 (MQ=255) taCTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGtt > 1:3492520/1‑70 (MQ=255) taCTTGGACCGCAAGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGttt > 1:1744438/1‑71 (MQ=255) cTTGGACCTCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGc > 1:1695849/1‑44 (MQ=255) cTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGtt > 1:1466697/1‑41 (MQ=255) cTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCg > 1:854968/1‑43 (MQ=255) cTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGc > 1:354046/1‑44 (MQ=255) cTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGc > 1:3216240/1‑44 (MQ=255) cTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGc > 1:523819/1‑44 (MQ=255) cTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGc > 1:4349745/1‑44 (MQ=255) cTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGc > 1:1511125/1‑44 (MQ=255) cTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGc > 1:3827752/1‑44 (MQ=255) cTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTtct > 1:2527000/1‑71 (MQ=255) cTTGGACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTtct > 1:4142643/1‑71 (MQ=255) ggACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTtctc < 1:3160654/69‑1 (MQ=255) ggACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTtctc < 1:16246/69‑1 (MQ=255) ggACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTtctc < 1:2059404/69‑1 (MQ=255) ggACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTtctc < 1:386739/69‑1 (MQ=255) ggACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTtctc < 1:4084459/69‑1 (MQ=255) ggACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTctct < 1:1903656/70‑1 (MQ=255) gACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTc < 1:4200813/71‑1 (MQ=255) gACCGCACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTc < 1:568620/71‑1 (MQ=255) | CTGCGGTTTACCGCCGGAAAGGGTGTGAAAACGTTTTGGACGCTTAATACGCGTCATATACTTGGACCACACGCGTTCTGCTTCTGTCACTGGCTCACGTTCGCCACGGCATACTGCTACGAAGAGTTTCTCTTC > minE/2450230‑2450364 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |