Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,473,718	C→T	F331F (TTC→TTT)	glmS →	L‑glutamine:D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,473,718	0	C	T	100.0%	185.7 / NA	56	F331F (TTC→TTT)	glmS	L‑glutamine:D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/26);  total (30/26)

GCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACCG  >  minE/2473660‑2473785                                                           |                                                                    gCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCaaa                                                         >  1:54387/1‑71 (MQ=255) gCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCaaa                                                         >  1:2741355/1‑71 (MQ=255) gCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCaaa                                                         >  1:2852966/1‑71 (MQ=255) gCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCaaa                                                         >  1:2504805/1‑71 (MQ=255) gCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCaaa                                                         >  1:2300193/1‑71 (MQ=255) gCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCaaa                                                         >  1:3269511/1‑71 (MQ=255) gCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCaaa                                                         >  1:1878471/1‑71 (MQ=255) gCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCaaa                                                         >  1:403352/1‑71 (MQ=255)       ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGcc                                                   >  1:4055261/1‑71 (MQ=255)       ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGcc                                                   >  1:50873/1‑71 (MQ=255)       ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGcc                                                   >  1:1251349/1‑71 (MQ=255)       ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGcc                                                   >  1:2387317/1‑71 (MQ=255)       ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGcc                                                   >  1:2310873/1‑71 (MQ=255)       ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGcc                                                   >  1:45800/1‑71 (MQ=255)       ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGcc                                                   >  1:3341479/1‑71 (MQ=255)       ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGcc                                                   >  1:3510330/1‑71 (MQ=255)       ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGcc                                                   >  1:3735963/1‑71 (MQ=255)       ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGcc                                                   >  1:1474316/1‑71 (MQ=255)        gTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGc                                                    <  1:3451827/69‑1 (MQ=255)        gTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGc                                                    <  1:1497051/69‑1 (MQ=255)             aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt                                             <  1:3513156/71‑1 (MQ=255)             aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt                                             <  1:482648/71‑1 (MQ=255)             aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt                                             <  1:4248343/71‑1 (MQ=255)             aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt                                             <  1:2902739/71‑1 (MQ=255)             aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt                                             <  1:2759602/71‑1 (MQ=255)             aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt                                             <  1:109470/71‑1 (MQ=255)             aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt                                             <  1:1080875/71‑1 (MQ=255)             aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt                                             <  1:261793/71‑1 (MQ=255)             aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt                                             <  1:249165/71‑1 (MQ=255)             aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt                                             <  1:1443090/71‑1 (MQ=255)             aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt                                             <  1:2286194/71‑1 (MQ=255)             aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt                                             <  1:1619708/71‑1 (MQ=255)             aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt                                             <  1:1771882/71‑1 (MQ=255)             aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt                                             <  1:2660251/71‑1 (MQ=255)             aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCAGTGcgt                                             <  1:4301719/71‑1 (MQ=255)              aTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt                                             <  1:3655225/70‑1 (MQ=255)              aTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt                                             <  1:862103/70‑1 (MQ=255)              aTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt                                             <  1:3294894/70‑1 (MQ=255)              aTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgt                                             <  1:2657826/70‑1 (MQ=255)                          tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtga                                >  1:3473194/1‑71 (MQ=255)                          tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtga                                >  1:2160443/1‑71 (MQ=255)                          tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtga                                >  1:2135494/1‑71 (MQ=255)                          tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtga                                >  1:2173536/1‑71 (MQ=255)                          tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtga                                >  1:2027910/1‑71 (MQ=255)                          tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtga                                >  1:3282435/1‑71 (MQ=255)                          tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtga                                >  1:4099883/1‑71 (MQ=255)                          tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtga                                >  1:830324/1‑71 (MQ=255)                          tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtga                                >  1:2993448/1‑71 (MQ=255)                          tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtga                                >  1:1412928/1‑71 (MQ=255)                          tATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtga                                >  1:1277378/1‑71 (MQ=255)                                                       aaTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCg        >  1:1103339/1‑66 (MQ=255)                                                        aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:2704931/71‑1 (MQ=255)                                                        aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:82241/71‑1 (MQ=255)                                                        aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:2516086/71‑1 (MQ=255)                                                        aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:4163137/71‑1 (MQ=255)                                                        aTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACcg  <  1:2187715/71‑1 (MQ=255)                                                           |                                                                    GCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAACCG  >  minE/2473660‑2473785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑